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Full Length Article | Open Access

Bioinformatic identification of key genes and molecular pathways in the spermatogenic process of cryptorchidism

Yu Zhoua,b,d, Deying Zhanga,b,c,d( ), Bo Liua,b,c, Dong Hua,b, Lianju Shenb,c,d, Chunlan Longb,c,d, Yihang Yua,b,c, Tao Lina,b,c, Xing Liua,b, Dawei Hea,b,c,d, Guanghui Weia,b,c,d( )
Department of Urology, Children's Hospital of Chongqing Medical University, Ministry of Education Key Laboratory of Child Development and Disorders, Chongqing 400014, China
Chongqing Key Laboratory of Children Urogenital Development and Tissue Engineering, China
China International Science and Technology Cooperation Base of Child Development and Critical Disorders, China
Chongqing Key Laboratory of Pediatrics, China

Peer review under responsibility of Chongqing Medical University.

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Abstract

This study aims to determine key genes and pathways that could play important roles in the spermatogenic process of patients with cryptorchidism. The gene expression profile data of GSE25518 was obtained from the Gene Expression Omnibus (GEO) database. Microarray data were analyzed using BRB-Array Tools to identify differentially expressed genes (DEGs) between high azoospermia risk (HAZR) patients and controls. In addition, other analytical methods were deployed, including hierarchical clustering analysis, class comparison between patients with HAZR and the normal control group, gene ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis, and the construction of a protein–protein interaction (PPI) network. In total, 1015 upregulated genes and 1650 downregulated genes were identified. GO and KEGG analysis revealed enrichment in terms of changes in the endoplasmic reticulum cellular component and the endoplasmic reticulum protein synthetic process in the HAZR group. Furthermore, the arachidonic acid pathway and mTOR pathway were also identified as important pathways, while RICTOR and GPX8 were indentified as key genes involved in the spermatogenic process of patients with cryptorchidism. In present study, we found that changes in the synthesis of endoplasmic reticulum proteins, arachidonic acid and the mTOR pathway are important in the incidence and spermatogenic process of cryptorchidism. GPX8 and RICTOR were also identified as key genes associated with cryptorchidism. Collectively, these data may provide novel clues with which to explore the precise etiology and mechanism underlying cryptorchidism and cryptorchidism-induced human infertility.

Keywords

BioinformaticsmTORArachidonic acid pathwayCryptorchidismDifferentially expressed genesGPX8RITORSpermatogenic

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Genes & Diseases
Volume 6 Issue 4,
December 2019
Pages 431-440
DOI: 10.1016/j.gendis.2018.11.002
Cite this article:
Zhou Y, Zhang D, Liu B, et al. Bioinformatic identification of key genes and molecular pathways in the spermatogenic process of cryptorchidism. Genes & Diseases, 2019, 6(4): 431-440. https://doi.org/10.1016/j.gendis.2018.11.002

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Received: 12 October 2018
Accepted: 07 November 2018
Published: 14 November 2018
© 2018, Chongqing Medical University.

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
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