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Research Article | Open Access

SRSFs mediate the function of AR in the ovarian granulosa cells of patients with PCOS

Jing Luoa,, Hong Yeb, Lijuan Haob, Yixuan Suna, Ruohan Lia, Yanxi Lia, Zhu Yanga( )
Gynecology and Obstetrics of the Second Affiliated Hospital of Chongqing Medical University, Chongqing, PR China
Gynecologic Endocrinology of Chongqing Health Center for Women and Children, Chongqing, PR China

Peer review under responsibility of Chongqing Medical University.

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Abstract

Ovarian hyperandrogenism is one of the characteristics of polycystic ovary syndrome (PCOS) and androgen receptor (AR) in ovarian granulosa cells (GCs) functions as an important element to the accumulation of androgens. This study verified the existence of alternative splicing variant of AR (AR-SVs) in the GCs of PCOS patients and found that the function of AR decreased significantly in the presence of AR-SVs. And compared to the normal individuals, the expression of Serine/arginine-rich splicing factor 2(SRSF2) was higher and the expression of SRSF3 was lower in the GCs of patients with AR-SVs. More importantly, we found that the expression of SRSF2 was inhibited and that the expression of AR was decreased after the successful upregulation of miRNA-183, and testostrone (T) concentrations in the culture medium were increased. The results also showed that the expression of SRSF3 decreased when miRNA-124 was successfully upregulated, while the expression of AR significantly increased; however, the function of AR was also inhibited when T concentration in the culture medium was increased. This study has proved that SRSFs are regulated by corresponding miRNAs, and the altered expression of SRSFs interferenced the alternative splicing process of AR and ultimately decreased the function of AR, leading to the accumulation of androgens in the ovary.

Keywords

miRNAsAndrogen receptorPCOSGranulosa cellsHyperandrogenismSRSFs

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Genes & Diseases
Volume 8 Issue 1,
January 2021
Pages 94-109
DOI: 10.1016/j.gendis.2019.09.005
Cite this article:
Luo J, Ye H, Hao L, et al. SRSFs mediate the function of AR in the ovarian granulosa cells of patients with PCOS. Genes & Diseases, 2021, 8(1): 94-109. https://doi.org/10.1016/j.gendis.2019.09.005

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Received: 20 April 2019
Revised: 26 August 2019
Accepted: 04 September 2019
Published: 17 September 2019
© 2019, Chongqing Medical University.

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
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