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Full Length Article | Open Access

Mitochondria-derived small RNAs as diagnostic biomarkers in lung cancer patients through a novel ratio-based expression analysis methodology

Zongtao Yua,b,1, Shaoqiu Chenc,d,1, Zhenming Tange,1, Ying Tange,1, Zhougui Linge( ), Hongwei Wangf, Ting Gongc,d, Zitong Gaoc,d, Gehan Devendrac,g, Gang Huangh( ), Wei Cheni( ), Youping Dengc( )
Department of Laboratory Medicine, Affiliated Taihe Hospital of Xi'an Jiaotong University Health Science Center, Shiyan, Hubei 710061, China
Department of Internal Medicine, Rush University Cancer Center, Rush University Medical Center, Kidston House, Chicago, IL 60612, USA
Department of Quantitative Health Sciences, John A. Burns School of Medicine, University of Hawaii at Manoa, Honolulu, HI 96813, USA
Molecular Biosciences and Bioengineering Program, College of Tropical Agriculture and Human Resources, The University of Hawaii at Manoa, Honolulu, HI 96822, USA
Department of Pulmonary and Critical Care Medicine, The Fourth Affiliated Hospital of Guangxi Medical University, Liuzhou, Guangxi 545005, China
Division of Research and Development, Decoding Therapeutics Corp, Mt Prospect, IL 60056, USA
Division Chief Pulmonary & Critical Care Medicine, The Queen’s Medical Center, John A Burns School of Medicine, Honolulu, HI 96813, USA
Shanghai Key Laboratory for Molecular Imaging, Shanghai University of Medicine and Health Sciences, Shanghai 201318, China
Department of Laboratory Medicine, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi 710061, China

Peer review under responsibility of Chongqing Medical University.

1 These authors contributed equally to this work.

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Abstract

Small non-coding RNAs are potential diagnostic biomarkers for lung cancer. Mitochondria-derived small RNA (mtRNA) is a novel regulatory small non-coding RNA that only recently has been identified and cataloged. Currently, there are no reports of studies of mtRNA in human lung cancer. Currently, normalization methods are unstable, and they often fail to identify differentially expressed small non-coding RNAs (sncRNAs). In order to identify reliable biomarkers for lung cancer screening, we used a ratio-based method using mtRNAs newly discovered in human peripheral blood mononuclear cells. In the discovery cohort (AUC = 0.981) and independent validation cohort (AUC = 0.916) the prediction model of eight mtRNA ratios distinguished lung cancer patients from controls. The prediction model will provide reliable biomarkers that will allow blood-based screening to become more feasible and will help make lung cancer diagnosis more accurate in clinical practice.

Keywords

Lung cancerMitochondria-derived small RNAsPlasmaRatioSmall non-coding RNAs

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Genes & Diseases
Volume 10 Issue 3,
May 2023
Pages 1055-1061
DOI: 10.1016/j.gendis.2022.07.013
Cite this article:
Yu Z, Chen S, Tang Z, et al. Mitochondria-derived small RNAs as diagnostic biomarkers in lung cancer patients through a novel ratio-based expression analysis methodology. Genes & Diseases, 2023, 10(3): 1055-1061. https://doi.org/10.1016/j.gendis.2022.07.013

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Received: 07 April 2022
Revised: 01 July 2022
Accepted: 16 July 2022
Published: 07 August 2022
© 2022 The Authors.

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
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