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Research Article | Open Access

Fractalkine/CX3CR1 pathway is neuroprotective in intracerebral hemorrhage through facilitating the expression of TGF-β1

Hai-Han Yu,a, Gai-Gai Li, Ying-Xin Tang, Shuang Bai, Chao Pan( ), Zhou-Ping Tang( )
Department of Neurology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China

a First author.

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Abstract

Pronounced inflammation after intracerebral hemorrhage (ICH) contributes to secondary brain injury, which seriously affects the prognosis of ICH patients. Fractalkine (FKN)/CX3CR1 pathway may play an important role in regulating inflammatory reactions after ICH. As resident immunocyte in brain, microglia is essential for inflammatory reactions in central nervous system. This study intends to explore the effect of FKN/CX3CR1 pathway on microglia after experimental ICH and its possible mechanisms. In mouse ICH models, FKN and TGF-β1 levels gradually increased and showed a positive correlation, both of which peaked at the third day after ICH model. Compared with ICH only, the ratio of s-FKN/m-FKN, microglia activation, and neuronal apoptosis were all decreased after injecting ADAM10 inhibitor (G1254023X) into the lateral ventricle. The expression of inflammatory factors were increased after stimulation with hemin in BV2 cells. The addition of m-FKN reduced the inflammatory response to a certain extent, and the inhibitor of TGF-β1 could counteract the effect of m-FKN.

In conclusion, in mouse ICH model, the expression of FKN is increased, and m-FKN may inhibit the activation of microglia and reduce neuron apoptosis by promoting the expression of TGF-β1.

Keywords

MicrogliaIntracerebral hemorrhageTGF-β1FractalkineBV2

References

1

Qureshi AI, Mendelow AD, Hanley DF. Intracerebral haemorrhage. Lancet. 2009;373(9675):1632–1644.
Crossref Google Scholar
2

Jiang B, Wang WZ, Chen H, et al. Incidence and trends of stroke and its subtypes in China: results from three large cities. Stroke. 2006;37(1):63–68.
Crossref Google Scholar
3

van Asch CJ, Luitse MJ, Rinkel GJ, van der Tweel I, Algra A, Klijn CJ. Incidence, case fatality, and functional outcome of intracerebral haemorrhage over time, according to age, sex, and ethnic origin: a systematic review and meta-analysis. Lancet Neurol. 2010;9(2):167–176.
Crossref Google Scholar
4

Schlunk F, Greenberg SM. The pathophysiology of intracerebral hemorrhage formation and expansion. Transl Stroke Res. 2015;6(4):257–263.
Crossref Google Scholar
5

Keep RF, Hua Y, Xi G. Intracerebral haemorrhage: mechanisms of injury and therapeutic targets. Lancet Neurol. 2012;11(8):720–731.
Crossref Google Scholar
6

Lee SH, Park HK, Ryu WS, et al. Effects of celecoxib on hematoma and edema volumes in primary intracerebral hemorrhage: a multicenter randomized controlled trial. Eur J Neurol. 2013;20(8):1161–1169.
Crossref Google Scholar
7

Fu Y, Hao J, Zhang N, et al. Fingolimod for the treatment of intracerebral hemorrhage: a 2-arm proof-of-concept study. Jama Neurol. 2014;71(9):1092–1101.
Crossref Google Scholar
8

Lauro C, Catalano M, Trettel F, Limatola C. Fractalkine in the nervous system: neuroprotective or neurotoxic molecule?. Ann Ny Acad Sci. 2015;1351(1):141–148.
Crossref Google Scholar
9

Liu W, Jiang L, Bian C, et al. Role of CX3CL1 in diseases. Arch Immunol Ther Ex. 2016;64(5):371–383.
Crossref Google Scholar
10

Cardona AE, Pioro EP, Sasse ME, et al. Control of microglial neurotoxicity by the fractalkine receptor. Nat Neurosci. 2006;9(7):917–924.
Crossref Google Scholar
11

Huang D, Shi FD, Jung S, et al. The neuronal chemokine CX3CL1/fractalkine selectively recruits NK cells that modify experimental autoimmune encephalomyelitis within the central nervous system. Faseb J. 2006;20(7):896–905.
Crossref Google Scholar
12

Roche SL, Wyse-Jackson AC, Ruiz-Lopez AM, Byrne AM, Cotter TG. Fractalkine-CX3CR1 signaling is critical for progesterone-mediated neuroprotection in the retina. Sci Rep-Uk. 2017;7:43067.
Crossref Google Scholar
13

Gan Y, Liu Q, Wu W, et al. Ischemic neurons recruit natural killer cells that accelerate brain infarction. Proc Natl Acad Sci U S A. 2014;111(7):2704–2709.
Crossref Google Scholar
14

Yang XP, Mattagajasingh S, Su S, et al. Fractalkine upregulates intercellular adhesion molecule-1 in endothelial cells through CX3CR1 and the Jak Stat5 pathway. Circ Res. 2007;101(10):1001–1008.
Crossref Google Scholar
15

Pan C, Liu N, Zhang P, et al. EGb761 ameliorates neuronal apoptosis and promotes angiogenesis in experimental intracerebral hemorrhage via RSK1/GSK3beta pathway. Mol Neurobiol. 2017.
Crossref Google Scholar
16

Li G, Yu H, Liu N, et al. Overexpression of CX3CR1 in adipose-derived stem cells promotes cell migration and functional recovery after experimental intracerebral hemorrhage. Front Neurosci-Switz. 2019;13.
Crossref Google Scholar
17

Lauro C, Di Angelantonio S, Cipriani R, et al. Activity of adenosine receptors type 1 Is required for CX3CL1-mediated neuroprotection and neuromodulation in hippocampal neurons. J Immunol. 2008;180(11):7590–7596.
Crossref Google Scholar
18

Dong L, Nordlohne J, Ge S, Hertel B, Melk A. T Cell CX3CR1 mediates excess atherosclerotic inflammation in renal impairment. J Am Soc Nephrol. 2016.
Crossref Google Scholar
19

Taylor RA, Chang C, Goods BA, et al. TGF-β1 modulates microglial phenotype and promotes recovery after intracerebral hemorrhage. J Clin Invest. 2017;127(1):280–292.
Crossref Google Scholar
Brain Hemorrhages
Volume 1 Issue 3,
September 2020
Pages 146-151
DOI: 10.1016/j.hest.2020.06.002
Cite this article:
Yu H-H, Li G-G, Tang Y-X, et al. Fractalkine/CX3CR1 pathway is neuroprotective in intracerebral hemorrhage through facilitating the expression of TGF-β1. Brain Hemorrhages, 2020, 1(3): 146-151. https://doi.org/10.1016/j.hest.2020.06.002

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Received: 15 April 2020
Revised: 06 June 2020
Accepted: 11 June 2020
Published: 10 July 2020
© 2020 International Hemorrhagic Stroke Association.

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
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