Abstract
This research investigated physiological and biochemical changes at rootstock–scion union of graft combinations between Iranian rootstocks and Asgari scion in response to NAA and BAP application. Grafting cuts of scion and rootstock were dipped into NAA (0, 200 and 400·mg L−1) and BAP (0, 250 and 500·mg L−1) solutions for 30·min, immediately after the cuttings were grafted using the wedge grafting technique. The highest successful grafting rate (82%) was recorded with Asgari–Asgari combination treated with 200·mg L−1 NAA·+·500·mg L−1 BAP. Treating the graft cut–surfaces with 200·mg L−1 NAA·+·500·mg L−1 BAP caused the lowest H2O2 and MDA contents. Among all the graft combinations, the highest increase in chlorophyll content with a high photosynthesis efficiency occurred in Asgari–Shahani when treated with 200·mg L−1 NAA·+·500·mg L−1 BAP. The graft union formation in the presence of 200·mg L−1 NAA·+·500·mg L−1 BAP application enhanced CAT and POX activities. In conclusion, treatment of graft zone with 200·mg L−1 NAA·+·500·mg L−1 BAP enhanced antioxidant activities and reduced lipid peroxidation in scion leaves. However, several grapevine rootstocks tested in our research were also impacting the scion performance.