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Research paper

Identification and Molecular Characterization of a Phytoplasma Associated with Pomegranate Fasciation Disease

Rui GAOa,b, Jie WANGc, Tiansheng ZHUd, Xi JIAe( ), Xiangdong LIb( )
Institute of Plant Protection, Shandong Academy of Agricultural Sciences, Ji'nan 250100, China
Department of Plant Pathology, College of Plant Protection, Shandong Agricultural University, Tai'an, Shandong 271018, China
Shandong Institute of Pomology, Tai'an, Shandong 271000, China
College of Plant science and Technology, Tarim University, Alar, Xinjiang 843300, China
Institute of Maize, Shandong Academy of Agricultural Sciences, Ji'nan 250100, China

Peer review under responsibility of Chinese Society for Horticultural Science (CSHS) and Institute of Vegetables and Flowers (IVF), Chinese Academy of Agricultural Sciences (CAAS)

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Abstract

To confirm phytoplasma infection, samples of pomegranate (Punica granatum L.) plants showing symptoms of fasciation were collected from an orchard located in Tai'an, Shandong Province, China. A fragment of approximately 1.2 kb was amplified with universal primers targeting the phytoplasma 16S rRNA gene from symptomatic pomegranate plants, while no fragment was obtained from healthy plants. The phytoplasma associated with the disease was designated as pomegranate fasciation (PoF). Two representative phytoplasma 16S rDNA gene sequences (PoF-Ch01 and PoF-Ch02) had 100% nucleotide sequence identity. The 16S rDNA sequence of PoF-Ch01 and PoF-Ch02 showed the highest similarity (99.6%) to that of ‘P. granatum’ phytoplasma isolate AY-PG, which belong to 16SrI-B. Further phylogenetic analysis showed that PoF-Ch01 and PoF-Ch02 belonged to a cluster of 16SrI subgroup members. In silico RFLP analysis indicated that PoF-Ch01 shared the highest similarity coefficient of 0.97 with reference strains of 16SrI-B, M and N. Actual RFLP analysis of both enzymes BstUI and BfaI confirmed that of the virtual RFLP analysis. Combining these results, we concluded that PoF was a member of the ‘Candidatus Phytoplasma asteris’ group (16SrI), and has very close relationship with 16SrI-B subgroup.

Keywords

Punica granatumfasciation diseaseRFLP analysis16SrI group

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Horticultural Plant Journal
Volume 4 Issue 1,
January 2018
Pages 30-34
DOI: 10.1016/j.hpj.2018.01.001
Cite this article:
GAO R, WANG J, ZHU T, et al. Identification and Molecular Characterization of a Phytoplasma Associated with Pomegranate Fasciation Disease. Horticultural Plant Journal, 2018, 4(1): 30-34. https://doi.org/10.1016/j.hpj.2018.01.001

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Received: 03 July 2017
Revised: 10 November 2017
Accepted: 12 December 2017
Published: 01 March 2018
© 2018 Chinese Society for Horticultural Science (CSHS) and Institute of Vegetables and Flowers (IVF), Chinese Academy of Agricultural Sciences (CAAS).

This is an open access article under the CC BY-NC-ND license. (http://creativecommons.org/licenses/by-nc-nd/4.0/)
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