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Research paper

Identification and Characterization of microRNA during Bemisia tabaci Infestations in Solanum lycopersicum and Solanum habrochaites

Ketao WANGa,b,1, Xiaomei SUa,1, Xia CUIa, Yongchen DUa, Shuaibin ZHANGa( ), Jianchang GAOa( )
Key Laboratory of Biology and Genetic Improvement of Horticultural Crops of the Ministry of Agriculture, Sino-Dutch Joint Laboratory of Horticultural Genomics, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China
College of Horticulture, China Agricultural University, Beijing 100193, China

1 These authors contributed equally to this work.

Peer review under responsibility of Chinese Society for Horticultural Science (CSHS) and Institute of Vegetables and Flowers (IVF), Chinese Academy of Agricultural Sciences (CAAS)

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Abstract

MicroRNAs (miRNAs) are a class of small non-coding RNAs that can regulate target gene expression during many plant growth and development processes. In recent years, several studies identified the miRNAs involved in fruit development, leaf development, and abiotic responses in tomato. However, little is known about the miRNAs that respond to insect attack. Here, miRNAs were identified by high-throughput sequencing at different stages after infections by the whitefly Bemisia tabaci in Solanum lycopersicum and Solanum habrochaites, which are susceptible and resistant to whitefly, respectively. A total of 44 known miRNA families were identified, and 33 were shared between the two species. Among these, 13 miRNA families were newly reported in tomato. After strict filtering, some novel miRNAs were also discovered. The global expression patterns of the miRNAs exhibited were different between the two species, reflecting their different responses and resistance levels to whitefly attack. Some of the predicted target genes of differentially expressed miRNAs may be involved in responding to, and defending against, diseases and insects. Thus, plant miRNAs are important in the responses to, and resistance against, insects and provide a useful resource for further investigations into the mechanism of miRNA-mediated plant–insect interactions.

Keywords

miRNAshigh-throughput sequencingSolanum lycopersicumSolanum habrochaitesBemisia tabaciwhitefly infection

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Horticultural Plant Journal
Volume 4 Issue 2,
March 2018
Pages 62-72
DOI: 10.1016/j.hpj.2018.03.002
Cite this article:
WANG K, SU X, CUI X, et al. Identification and Characterization of microRNA during Bemisia tabaci Infestations in Solanum lycopersicum and Solanum habrochaites. Horticultural Plant Journal, 2018, 4(2): 62-72. https://doi.org/10.1016/j.hpj.2018.03.002

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Received: 08 August 2017
Revised: 18 October 2017
Accepted: 25 December 2017
Published: 07 March 2018
© 2018 Chinese Society for Horticultural Science (CSHS) and Institute of Vegetables and Flowers (IVF), Chinese Academy of Agricultural Sciences (CAAS).

This is an open access article under the CC BY-NC-ND license. (http://creativecommons.org/licenses/by-nc-nd/4.0/)
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