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Research paper

Cytological and Molecular Characteristics of Pollen Abortion in Lily with Dysplastic Tapetum

Xueqian Wanga,b, Ze Wua,b, Lanqing Wanga,b, Meijiao Wua,b, Dehua Zhanga,b, Weimin Fanga, Fadi Chena, Nianjun Tenga,b( )
College of Horticulture, Nanjing Agricultural University/Key Laboratory of Landscape Design, Ministry of Agriculture and Rural Affairs, Nanjing 210095, China
Baguazhou Science and Technology Innovation Center of Modern Horticultural Industry, Nanjing 210043, China

Peer review under responsibility of Chinese Society for Horticultural Science (CSHS) and Institute of Vegetables and Flowers (IVF), Chinese Academy of Agricultural Sciences (CAAS)

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Abstract

Lily was grown worldwide as a fresh cutting flower because of its colorful petals, but its anther contained a large number of pollen grains that cause serious pollen contamination, however, pollen abortion can effectively reduce the level of pollen pollution. Our analysis aims to use cytological observation to detect the critical stage when pollen abortion occurs and to provide comprehensive gene expression information at the transcriptional level. The result showed that pollen abortion in ‘Little Kiss’ began at the mononuclear stage and the callose that covers the microspores failed to degenerate when young pollens were released from the tetrads. In addition, compared with the normally developed one, the tapetum of ‘Little Kiss’ degraded in advance while the degradation of callose was delayed. Furthermore, 103 differentially expressed genes (DEGs) related to the advance degeneration of tapetum cells and callose were found in the expression levels, including 22 transcription factors (TFs). In particular, two β-glucanase genes (endo-1,3(4)-β-glucanase, exo-β-glucanase) responsible for callose degeneration were significantly down-regulated. These results suggested that pollen abortion may occur at mononuclear stage and that early degeneration of tapetum cells resulted in a significant down-regulation of β-glucanase genes. As a result, the callose to cover microspores impedes the formation of pollen walls, which may possibly lead to pollen abortion.

Keywords

transcription factorcalloseprogrammed cell deathtapetumLilium Asiatic hybridsmicrospore development

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Horticultural Plant Journal
Volume 5 Issue 6,
November 2019
Pages 281-294
DOI: 10.1016/j.hpj.2019.11.002
Cite this article:
Wang X, Wu Z, Wang L, et al. Cytological and Molecular Characteristics of Pollen Abortion in Lily with Dysplastic Tapetum. Horticultural Plant Journal, 2019, 5(6): 281-294. https://doi.org/10.1016/j.hpj.2019.11.002

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Received: 07 June 2019
Revised: 19 August 2019
Accepted: 12 October 2019
Published: 23 November 2019
© 2019 Chinese Society for Horticultural Science (CSHS) and Institute of Vegetables and Flowers (IVF), Chinese Academy of Agricultural Sciences (CAAS).

This is an open access article under the CC BY-NC-ND license. (http://creativecommons.org/licenses/by-nc-nd/4.0/)
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