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Research paper

Comprehensive genomic analysis and expression profiling of cysteine-rich polycomb-like transcription factor gene family in tea tree

Hong Nana,b, Yanglei Lina,b, Xinghua Wangc( ), Lizhi Gaoa,d( )
Plant Germplasm and Genomics Center, Germplasm Bank of Wild Species in Southwest China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China
University of the Chinese Academy of Sciences, Beijing 100039, China
Yunnan Pu'er Tea Tree Breeding Station, Pu Er 665099, China
Institution of Genomics and Bioinformatics, South China Agricultural University, Guangzhou 510642, China
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Abstract

Cysteine-rich polycomb-like (CPP) is a small gene family in plants, which plays key role in plant development and stress response. Although CPP transcription factors have been characterized in several other plant species, a genome-wide characterization of the CPP gene family has been absent in Camellia sinensis. In this study, we totally identified 7, 8, and 8 non-redundant CsCPP genes in three published genomes, including Camellia sinensis var. assamica cv. Yunkang-10 (CSA-YK10), Camellia sinensis var. sinensis cv. Biyun (CSS-BY) and Camellia sinensis var. sinensis cv. Shuchazao (CSS-SCZ). CPP proteins from tea tree and other plant species were classified into three groups, which were further divided into four subgroups based on phylogenetic relationships. Most CPP genes in the same subgroup had similar gene structures and conserved motifs. The cis-acting elements analysis indicated that CPP genes might be involved in plant growth, development and stress responses. Analysis of gene expression using qRT-PCR experiments validated that CPP genes exhibited different expression patterns across the examined tissues. All the genes were expressed differentially in a range of tissues, indicating that CPPs were involved in a range of developmental and physiological processes. This study has obtained new insights into the evolution and function of the CPP gene family in the growth and development of tea plants, and also provide candidate genes for further functional characterization in tea tree.

Keywords

Camellia sinensisCPP transcription factorGenome-wide analysisGene expression profiling

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Horticultural Plant Journal
Volume 7 Issue 5,
September 2021
Pages 469-478
DOI: 10.1016/j.hpj.2021.03.001
Cite this article:
Nan H, Lin Y, Wang X, et al. Comprehensive genomic analysis and expression profiling of cysteine-rich polycomb-like transcription factor gene family in tea tree. Horticultural Plant Journal, 2021, 7(5): 469-478. https://doi.org/10.1016/j.hpj.2021.03.001

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Received: 06 April 2020
Revised: 04 June 2020
Accepted: 10 July 2020
Published: 11 March 2021
© 2021 Chinese Society for Horticultural Science (CSHS) and Institute of Vegetables and Flowers (IVF), Chinese Academy of Agricultural Sciences (CAAS). Publishing services by Elsevier B.V. on behalf of KeAi Communications Co. Ltd.

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/)
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