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Research paper

Transcriptomics‐based identification and characterization of genes related to sugar metabolism in 'Hongshuijing' pitaya

Zhike Zhanga,1, Yemiao Xinga,1, Muthusamy Ramakrishnanb,c, Canbin Chena, Fangfang Xiea, Qingzhu Huaa, Jianye Chena, Rong Zhanga, Jietang Zhaoa, Guibing Hua, Yonghua Qina( )
State Key Laboratory for Conservation and Utilization of Subtropical Agrobioresources/ Guangdong Provincial Key Laboratory of Postharvest Science of Fruits and Vegetables/Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (South China), Ministry of Agriculture and Rural Affairs, College of Horticulture, South China Agricultural University, Guangzhou 510642, China
Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210037, China
Bamboo Research Institute, Nanjing Forestry University, Nanjing 210037, China

1 These authors contributed equally to this work.

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Abstract

Sugar composition not only affects fruit flavor but is also an important determinant of fruit taste and consumer preference. In this study, changes in the sugar content and sugar-metabolizing enzymes were investigated from different sections of various fruit development phases of 'Hongshuijing' pitaya (Hylocereus monacanthus). Genes related to sugar metabolism were also screened by transcriptome analyses. The results indicated that glucose was the major sugar in mature pitaya fruit, and was mainly regulated by vacuolar acid invertase (VAI) and sucrose synthase (SS) (degradative direction). Sugar accumulation varied in pulp between different sections of the pitaya fruit. VAI, neutral invertase (NI) and SS (degradative direction) are crucial enzymes for sugar accumulation in pitaya. The expression of 17 genes related to sucrose metabolism obtained from seven databases [NCBI non-redundant protein database (Nr), NCBI non-redundant nucleotide sequence database (Nt), EuKaryotic Orthologous Groups (KOG), The Protein Families (Pfam), Kyoto Encyclopedia of Genes and Genomes (KEGG), Swiss-prot, and Gene Ontology (GO)] were analyzed in different pitaya pulp sections. HpVAI1 had the highest relative expression level on the 29th day after pollination (DAP). Positive correlations were found between HpVAI1 expression and VAI activity; HpNI4 and NI activity; HpSS2, HpSS5, and SS activity (synthetic direction), indicating that HpVAI1, HpNI4, and HpSS2 and HpSS5 were involved in the regulation of VAI, NI, and SS (synthetic direction), respectively. HpVAI1 and HpNI4 regulated sucrose degradation and the accumulation of glucose and fructose, while HpSS2 and HpSS5 regulated sucrose synthesis. These results suggest that HpVAI1 plays a key role in sugar metabolism during fruit development of 'Hongshuijing' pitaya. The results of this study provide new information about sugar metabolism in pitaya fruit that could help improve fruit quality and the breeding of new cultivars.

Keywords

Sugar metabolismqRT-PCRExpression analysisHylocereus monacanthusTranscriptional analysis

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Horticultural Plant Journal
Volume 8 Issue 4,
July 2022
Pages 450-460
DOI: 10.1016/j.hpj.2021.06.004
Cite this article:
Zhang Z, Xing Y, Ramakrishnan M, et al. Transcriptomics‐based identification and characterization of genes related to sugar metabolism in 'Hongshuijing' pitaya. Horticultural Plant Journal, 2022, 8(4): 450-460. https://doi.org/10.1016/j.hpj.2021.06.004

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Received: 18 February 2021
Revised: 19 May 2021
Accepted: 11 June 2021
Published: 20 August 2021
© 2022 Chinese Society for Horticultural Science (CSHS) and Institute of Vegetables and Flowers (IVF), Chinese Academy of Agricultural Sciences (CAAS).

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/)
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