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Original Article | Open Access

Paeoniflorin inhibits lipopolysaccharide-induced inflammation in LO2 cells by regulating RhoA/NLRP3 pathway

Haixia Liu1, Fafeng Cheng1, Feifei Tang, Yifang Wang, Shuling Liu, Xueqian Wang( )
School of Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing, 102488, China

Peer review under responsibility of Beijing University of Chinese Medicine.

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Abstract

Background

Inflammation is an essential component of liver diseases. Paeoniflorin (PF), a monoterpenoid component derived from peony root (Paeonia lactiflora Pall.), has anti-inflammatory, immunoregulatory, and hepatoprotective activities. However, whether PF affects liver inflammation and its underlying mechanisms is unclear. In this study, we investigated the effects of PF on lipopolysaccharide (LPS)-induced inflammation in LO2 cells and the underlying molecular mechanism.

Methods

LPS was used to induce inflammation. After PF pretreatment for 2 h, the cells were treated with PF and LPS. Cell counting kit-8 was used to measure cell viability. Tumor necrosis factor-α (TNF-α) and interleukin (IL)-6 were tested by Enzyme-linked immunosorbent assay. Western blot was used to evaluate TNF-α, Ras homolog family member A (RhoA), NOD-, LRR- and pyrin domain-containing protein 3 (NLRP3), apoptosis-associated speck-like protein containing a CARD (ASC), caspase-1, and IL-1β proteins expression.

Results

In LPS-induced LO2 cells, PF reduced TNF-α and IL-6 inflammatory cytokine production in a dose-dependent manner. LPS-induced TNF-α expression was also suppressed by PF. In addition, PF significantly inhibited LPS-induced RhoA activation (P = .0014). Finally, PF suppressed LPS-induced NLRP3 inflammasome activation by downregulating NLRP3, ASC, caspase-1, and IL-1β expression.

Conclusion

These findings suggest that PF alleviates inflammation induced by LPS and further suggest the anti-inflammatory effect of PF may follow via reduced RhoA and NLRP3 inflammasome activity.

Keywords

InflammationPaeoniflorinLipopolysaccharideRhoANLRP3 inflammasome

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Journal of Traditional Chinese Medical Sciences
Volume 8 Issue 2,
April 2021
Pages 161-165
DOI: 10.1016/j.jtcms.2021.06.001
Cite this article:
Liu H, Cheng F, Tang F, et al. Paeoniflorin inhibits lipopolysaccharide-induced inflammation in LO2 cells by regulating RhoA/NLRP3 pathway. Journal of Traditional Chinese Medical Sciences, 2021, 8(2): 161-165. https://doi.org/10.1016/j.jtcms.2021.06.001

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Received: 20 March 2021
Revised: 16 June 2021
Accepted: 16 June 2021
Published: 20 June 2021
© 2021 Beijing University of Chinese Medicine.

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
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