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Original Article | Open Access

Effects of different acupuncture manipulations on protein expression in the parietal cortex of spontaneously hypertensive rats

Jiaojuan Wua,1, Tianxiao Zenga,1, Jingrong Liangb, Xudong Zhanga, Qi Xiea, Taotao Lva, Pui Yee Wongc, Zhi Jid, Qingguo Liua( )
School of Acupuncture-Moxibustion and Tuina, Beijing University of Chinese Medicine, Beijing, 102488, China
Department of Pain Management, Huazhong University of Science and Technology Union Shenzhen Hospital, Shenzhen, 518052, China
Shu Guang TCM Healthcare Center, Kuala Lumpur, 56000, Malaysia
Department of Acupuncture-Moxibustion, China-Japan Friendship Hospital, Beijing, 100029, China

Peer review under responsibility of Beijing University of Chinese Medicine.

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Abstract

Objective

To analyze the effects of twirling reinforcing and reducing manipulations on protein expression in parietal cortex of spontaneously hypertensive rats (SHRs), and elucidate the main mechanisms and differences between two manipulations in hypertension treatment.

Methods

Rats were randomly divided into the control, model, twirling reinforcing manipulation (TRFM), and twirling reducing manipulation (TRDM) groups. The control and model groups received catch and fixation stimulations once a day for 14 days. The TRFM and TRDM groups were intervened once a day for 20 min for 14 days. On days 0, 2, 6, 10, and 14 after acupuncture, rat systolic blood pressures (SBPs) were measured. Differential protein (DP) expression in the rat parietal cortices was detected. Thereafter, GO functional significance and KEGG pathway enrichment analyses were performed.

Results

Compared with the model group, SBP of rats in the TRDM and TRFM groups decreased on days 6 and 10 of acupuncture, respectively (P = .009; P < .001). Moreover, SBP of the TRDM group was significantly lower than that of the TRFM group on days 10 and 14 of acupuncture (P = .015; P = .013). Compared with control group, 601 and 1040 DPs were up- and downregulated, respectively, in the model group. Compared with model group, 44 and 28 up- and downregulated DPs were expressed, respectively, in the TRFM group. Compared with model group, expression of 616 and 427 up- and downregulated DPs, respectively, was found in the TRDM group. After combining the results of GO and KEGG enrichment analyses, five and one pathways were found to be related to the central antihypertensive mechanism of the parietal cortex during twirling reducing and reinforcing manipulations, respectively.

Conclusion

TRDM showed a more effective antihypertensive effect on SHRs than TRFM; this antihypertensive effect was related to the regulation of different proteins and their biological functions.

Keywords

AcupunctureHypertensionManipulationProteomicsParietal cortex

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Journal of Traditional Chinese Medical Sciences
Volume 8 Issue 3,
July 2021
Pages 257-264
DOI: 10.1016/j.jtcms.2021.07.009
Cite this article:
Wu J, Zeng T, Liang J, et al. Effects of different acupuncture manipulations on protein expression in the parietal cortex of spontaneously hypertensive rats. Journal of Traditional Chinese Medical Sciences, 2021, 8(3): 257-264. https://doi.org/10.1016/j.jtcms.2021.07.009

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Received: 09 March 2021
Revised: 06 July 2021
Accepted: 07 July 2021
Published: 17 July 2021
© 2021 Beijing University of Chinese Medicine.

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
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