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Original Article | Open Access

Anti-melanoma action of small molecular peptides derived from Brucea javanica (L.) Merr. globulin in vitro

Yi Zhaoa,1, Huiyun Wanga,1,1, Yanyan Yina, Haoyu Shia, Dong Wangb, Fengjue Shuc, Rongchun Wangd, Lingzhi Wanga( )
School of Life Sciences, Beijing University of Chinese Medicine, Beijing, 102488, China
School of Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing, 102488, China
Karna Limited Liability Company, Atlanta, 30345, USA
Biology Institute, Qilu University of Technology (Shandong Academy of Sciences), Jinan, 250103, China

Peer review under responsibility of Beijing University of Chinese Medicine.

1 These authors have contributed equally to this work.

Show Author Information

Abstract

Objective

The morbidity of malignant melanoma keeps increasing annually. It has high risks of metastasis, drug resistance, and poor prognosis in clinics. Moreover, the available medicines used commonly, such as dacarbazine, temozolomide, the v-Raf murine sarcoma viral oncogene homolog B1 (BRAF) inhibitor vemurafenib, and the programmed cell death protein 1 inhibitor pembrolizumab, have some limitations at some extent. Therefore, a more effective therapeutic strategy is still urgently necessary.

Methods

In this study, Brucea javanica(L.)Merr. globulins were hydrolyzed with pepsin, then ultra-filtrated to collect small molecular peptides (≤3 kDa). The peptides were then analyzed by anti-proliferative assay, cell-cycle distribution, apoptosis assay, and in vitro wound-scratch assay. Finally, western blotting was conducted to elucidate the underlying anti-melanoma mechanism.

Results

The small molecular peptide from B. javanica significantly inhibited malignant melanoma cell proliferation with the IC50 of 2.72 μg/mL for 72 h. Further analysis indicated that B. javanica peptides arrested cell cycle at the S and G2/M phases and induced apoptosis by upregulating p21, p53, Bax, caspase-3, and cleaved PARP while downregulating Bcl-2 expression. The inhibitory migration effects were also confirmed by wound-healing assay.

Conclusion

The small molecular biopeptides from B. javanica may be a promising bioactive agent candidate for melanoma treatment.

Keywords

PeptideMelanomaBrucea javanica (L.) Merr.GlobulinIn vitro wound-scratch assayCell-cycle assayApoptosis assayHydrolyze

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Journal of Traditional Chinese Medical Sciences
Volume 9 Issue 1,
January 2022
Pages 85-91
DOI: 10.1016/j.jtcms.2022.01.001
Cite this article:
Zhao Y, Wang H, Yin Y, et al. Anti-melanoma action of small molecular peptides derived from Brucea javanica (L.) Merr. globulin in vitro. Journal of Traditional Chinese Medical Sciences, 2022, 9(1): 85-91. https://doi.org/10.1016/j.jtcms.2022.01.001

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Received: 03 October 2021
Revised: 31 December 2021
Accepted: 01 January 2022
Published: 04 January 2022
© 2022 Beijing University of Chinese Medicine.

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
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