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Original Article | Open Access

Liangxue Xiaoban decoction and its disassembled prescriptions ameliorate psoriasis-like skin lesions induced by imiquimod in mice via T cell regulation

Feifei Tang, Shuling Liu, Fafeng Cheng, Qingguo Wang, Xueqian Wang( )
School of Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing, 102488, China

Peer review under responsibility of Beijing University of Chinese Medicine.

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Abstract

Objective

To explore the therapeutic capacity of the Liangxue Xiaoban (LXXB) decoction and its disassembled prescriptions in the modulation of T cell subsets and recurrence-related indexes of psoriasis using a psoriasis-like mouse model.

Methods

The psoriasis model was generated by the treatment of BALB/c mice (n = 48) with imiquimod. Mice were divided into six groups: control, psoriasis model, tripterygium glycosides, LXXB decoction, Liangxue decoction, and Qingqi decoction. After the intervention period, the interleukin (IL)-17A, IL-22, and interferon-γ levels in mice were examined and hematoxylin and eosin staining was conducted to determine pathological changes in the skin tissues. T cell subset changes in the skin-draining lymph nodes were analyzed using flow cytometry, and the expression levels of the associated transcription factors and recurrence-related indexes in the skin tissues were determined using a polymerase chain reaction.

Results

LXXB decoction attenuated the levels of CD8+ T, Th17, and Th1 cells and induced an increase in the Th2 and Treg cell levels. The disassembled prescriptions promoted or inhibited specific subsets of T cells to improve the symptoms of psoriasis. Notably, the LXXB and Liangxue decoctions suppressed the expression of IL-22 at both the gene and protein levels and restored the CD103 and IL-15 expressions in the skin tissue to the normal range.

Conclusion

LXXB decoction exerted significant immunoregulatory effects on T cell subsets and improved the recurrence-related indexes. Interestingly, the Liangxue prescription appeared to have a therapeutic advantage in terms of Th17 modulation and psoriasis recurrence, while the Qingqi prescription performed better in Treg immunoregulation.

Keywords

RecurrencePsoriasisT cellsLiangxue Xiaoban decoctionDisassembled prescriptionTripterygium glycoside

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Journal of Traditional Chinese Medical Sciences
Volume 9 Issue 4,
October 2022
Pages 409-419
DOI: 10.1016/j.jtcms.2022.09.003
Cite this article:
Tang F, Liu S, Cheng F, et al. Liangxue Xiaoban decoction and its disassembled prescriptions ameliorate psoriasis-like skin lesions induced by imiquimod in mice via T cell regulation. Journal of Traditional Chinese Medical Sciences, 2022, 9(4): 409-419. https://doi.org/10.1016/j.jtcms.2022.09.003

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Received: 29 June 2022
Revised: 12 September 2022
Accepted: 12 September 2022
Published: 15 September 2022
© 2022 Beijing University of Chinese Medicine.

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
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