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Original Article | Open Access

A hippocampal anti-hypertensive mechanism induced by twirling reinforcing-reducing manipulation in rats

Jiao Suna,1, Liu Yanb,1, Yunhong Zhub, Jingrong Lianga, Yingying Zanga, Tianxiao Zenga, Xiaomin Haoa, Pui Yee Wongc, Qingguo Liua( )
School of Acupuncture-Moxibustion and Tuina, Beijing University of Chinese Medicine, Beijing 102488, China
Department of Rehabilitation, Qingdao Hospital of Traditional Chinese Medicine (Qingdao Hiser Hospital), Qingdao 266033, China
Department of Acupuncture, Mediseed Chinese Medicine Consulting Centre, Petaling Jaya 46200, Malaysia

Peer review under responsibility of Beijing University of Chinese Medicine.

1 These authors have contributed equally to this work.

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Abstract

Objective

To investigate a hippocampal anti-hypertensive mechanism induced by twirling reinforcing-reducing manipulation (TRRM) using proteomics in rats.

Methods

Forty-two male spontaneously hypertensive rats were randomly divided into 3 groups, and 14 Wistar-Kyoto rats were served as control group. In the twirling reinforcing (TRF) group, needles were directly inserted into the Taichong (LR 3) point, then thumbs were moved heavily forward and lightly backward for 3 min, while needles remained inserted for 17 min. In the twirling reduction (TRD) group, the same treatment was applied as in the TRF group except that the thumb moved lightly forward and heavily backward. In the model and control groups, only the corresponding grasping and fixation were applied. All interventions were conducted for 14 days. The blood pressure (BP) of all rats was measured one day before intervention and every other day after. Then hematoxylin-eosin (H&E) staining, label-free and parallel reaction monitoring proteomic techniques were used to assess hippocampal samples from each group.

Results

Systolic BPs showed a significant decrease in the TRF and TRD groups compared with the model group (P < .01). In the model group, H&E staining showed obvious pathological changes in the hippocampus, while in the TRF and TRD groups, the hippocampal morphology was only slightly altered. Label-free proteomic analysis revealed 1163 differential protein expressions between groups. Gene Ontology enrichment analysis confirmed that the differentially expressed proteins were enriched in different biological processes, cellular components, and molecular functions. Kyoto Encyclopedia of Genes and Genomes enrichment analysis showed that TRRM proteins were expressed in the serotonergic synapse pathway, renin-angiotensin system, the mitogen-activated protein kinase signaling pathway, and the peroxisome pathway, which were all also related to BP regulation.

Conclusion

TRRM can significantly lower the BP of SHRs. The mode of action may be through the activation of various protein pathways in the hippocampus that are related to BP regulation.

Keywords

HippocampusAcupunctureHypertensionProteomicsBlood pressureTwirling reinforcing-reducing methodSpontaneously hypertension ratsTaichong

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Journal of Traditional Chinese Medical Sciences
Volume 10 Issue 1,
January 2023
Pages 106-117
DOI: 10.1016/j.jtcms.2022.11.006
Cite this article:
Sun J, Yan L, Zhu Y, et al. A hippocampal anti-hypertensive mechanism induced by twirling reinforcing-reducing manipulation in rats. Journal of Traditional Chinese Medical Sciences, 2023, 10(1): 106-117. https://doi.org/10.1016/j.jtcms.2022.11.006

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Received: 24 February 2022
Revised: 25 November 2022
Accepted: 26 November 2022
Published: 02 December 2022
© 2023 Beijing University of Chinese Medicine.

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
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