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Original Article | Open Access

Exploring the anti-aging effects of chlorogenic acid and the underlying mechanisms based on a Caenorhabditis elegans model

Yuqian Yanga, Xu Chena, Danyang Yea, Chuanyu Weib, Xinxin Panga, Chuchu Konga, Yongsheng Fanga, Hongliu Yanga, Yuanyuan Zhanga, Yonggang Liua( )
School of Chinese Materia Madica, Beijing University of Chinese Medicine, Beijing, 102488, China
Krannert Institute of Cardiology, Indiana University, Burmington, Indiana, 46517, USA

Peer review under responsibility of Beijing University of Chinese Medicine.

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Abstract

Objective

To explore the anti-aging effects of chlorogenic acid (CGA) and the underlying mechanisms based on a Caenorhabditis elegans (C. elegans) model.

Methods

The anti-aging activity of CGA was studied based on the body length, exercise behavior, lipofuscin content, antioxidative stress ability, swallowing frequency, body-bending frequency, and head-swinging ability of C. elegans. Through DAF-16 nuclear translocation and SOD-3-GFP fluorescence experiments, the effects of CGA on ROS levels, antioxidant enzyme activities, MDA content, mutant-strain lifespan, and anti-aging molecular signaling pathways were explored, as well as the underlying mechanisms.

Results

CGA improved multiple indices of the nematode: body length was increased (all P < .001), head-swing frequency and body-bending frequency were increased (all P < .05), nematode longevity was prolonged (P = .0021), lipofuscin deposition in nematodes was slowed down (all P < .001), the chemotaxis index was improved (P = .0012), ROS levels were reduced (all P < .001), and SOD activity and MDA content were reduced (SOD: P = .0017 between the low-concentration group and the control group, P < .001 between the high-concentration and medium-concentration groups and the control group; MDA: P = .0135 between the low-concentration group and the control group, and P < .001 between the high-concentration and medium-concentration groups and the control group). In addition, CGA also activated the DAF-16 transcription factor, promoted DAF-16 nuclear translocation under oxidative stress conditions (both P < .001 between the high-concentration and medium-concentration groups and the control group), and increased SOD-3 gene expression in nematodes (all P < .001).

Conclusion

CGA plays an anti-aging role in C. elegans. The underlying mechanisms include activation of the insulin/IGF-1 signaling pathway and enhancement of DAF-16 activity. This study lays a foundation for further research into the anti-aging effects of CGA.

Keywords

Antioxidant activityAnti-agingCaenorhabditis elegansChlorogenic acidDAF-16

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Journal of Traditional Chinese Medical Sciences
Volume 10 Issue 2,
April 2023
Pages 208-217
DOI: 10.1016/j.jtcms.2023.02.003
Cite this article:
Yang Y, Chen X, Ye D, et al. Exploring the anti-aging effects of chlorogenic acid and the underlying mechanisms based on a Caenorhabditis elegans model. Journal of Traditional Chinese Medical Sciences, 2023, 10(2): 208-217. https://doi.org/10.1016/j.jtcms.2023.02.003

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Received: 09 August 2022
Revised: 08 February 2023
Accepted: 09 February 2023
Published: 16 February 2023
© 2023 Beijing University of Chinese Medicine.

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
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