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Original Article | Open Access

GPCR-Gs mediates the protective effects of ginsenoside Rb1 against oxygen-glucose deprivation/re-oxygenation-induced astrocyte injury

Xi Wanga, Ying Liua, Juan Lib, Jiayu Xiea, Yi Daic, Minke Tanga,( )
School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 102488, China
Taihe Hospital, Hubei University of Medicine, Shiyan 442000, China
School of Medicine, Hyogo Medical University, Nishinomiya 663-8501, Japan

Peer review under responsibility of Beijing University of Chinese Medicine.

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Abstract

Objectives

To investigate whether the protective actions of ginsenoside Rb1 (Rb1) on astrocytes are mediated through the Gs-type G-protein-coupled receptor (GPCR-Gs).

Methods

Primary astrocyte cultures derived from neonatal mouse brain were used. Astrocyte injury was induced via oxygen-glucose deprivation/re-oxygenation (OGD/R). Cell morphology, viability, lactate dehydrogenase (LDH) leakage, apoptosis, glutamate uptake, and brain-derived neurotrophic factor (BDNF) secretion were assessed to gauge cell survival and functionality. Western blot was used to investigate the cyclic adenosine monophosphate (cAMP) and protein kinase B (Akt) signaling pathways. GPCR-Gs-specific inhibitors and molecular docking were used to identify target receptors.

Results

Rb1 at concentrations ranging from 0.8 to 5 μM did not significantly affect the viability, glutamate uptake, or BDNF secretion in normal astrocytes. OGD/R reduced astrocyte viability, increasing their LDH leakage and apoptosis rate. It also decreased glutamate uptake and BDNF secretion by these cells. Rb1 had protective effects of astrocytes challenged by OGD/R, by improving viability, reducing apoptosis, and enhancing glutamate uptake and BDNF secretion. Additionally, Rb1 activated the cAMP and Akt pathways in these cells. When the GPCR-Gs inhibitor NF449 was introduced, the protective effects of Rb1 completely disappeared, and its activation of cAMP and Akt signaling pathways was significantly inhibited.

Conclusion

Rb1 protects against astrocytes from OGD/R-induced injury through GPCR-Gs mediation.

Keywords

AstrocytesNeuroprotective effectsReceptorGinsenoside Rb1GinsengGPCR

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Journal of Traditional Chinese Medical Sciences
Volume 11 Issue 1,
January 2024
Pages 33-43
DOI: 10.1016/j.jtcms.2023.11.002
Cite this article:
Wang X, Liu Y, Li J, et al. GPCR-Gs mediates the protective effects of ginsenoside Rb1 against oxygen-glucose deprivation/re-oxygenation-induced astrocyte injury. Journal of Traditional Chinese Medical Sciences, 2024, 11(1): 33-43. https://doi.org/10.1016/j.jtcms.2023.11.002

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Received: 28 September 2023
Revised: 13 November 2023
Accepted: 13 November 2023
Published: 18 November 2023
© 2024 Beijing University of Chinese Medicine.

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
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