Effects of intravaginal curcumin gels combined with electroporation on vulvovaginal candidiasis
)Abstract
To observed the effect of a curcumin-based vaginal gel combined with electroporation for the treatment of vulvovaginal candidiasis (VVC) caused by Candida albicans.
Temperature-sensitive in situ gels (ISG) were prepared using poloxamers 407 and 188 as matrices. The mass ratio of poloxamer 407 and poloxamer 188 was 7:1 with a gelation temperature of approximately 29℃ and gelation time of 2.5 min.
Electroporation increased the transmucosal permeability of the model drug, doxorubicin and improved the antifungal effects of curcumin. In vitro antifungal experiments showed that the number of fungal colonies in curcumin ISG combined with electroporation was lower than that in pure curcumin ISG. In vivo pharmacodynamic experiments showed that, compared to the model group, curcumin ISG with electroporation inhibited the growth of C. albicans, alleviated vaginal mucosal edema, and reduced the inflammatory response.
Curcumin ISG combined with electroporation has substantial potential for the efficient clinical treatment of VVC.
References
Marnach ML, Wygant JN, Casey PM. Evaluation and management of vaginitis. Mayo Clin Proc. 2022;97(2):347-358.
Cooke G, Watson C, Deckx L, Pirotta M, Smith J, van Driel ML. Treatment for recurrent vulvovaginal candidiasis (thrush). Cochrane Database Syst Rev. 2022;1(1):CD009151.
Rodríguez-Cerdeira C, Martínez-Herrera E, Carnero-Gregorio M, et al. Pathogenesis and clinical relevance of Candida biofilms in vulvovaginal candidiasis. Front Microbiol. 2020;11:544480.
Yano J, Sobel JD, Nyirjesy P, et al. Current patient perspectives of vulvovaginal candidiasis: incidence, symptoms, management and post-treatment outcomes. BMC Wom Health. 2019;19(1):48.
Ben-Ami R, Kontoyiannis DP. Resistance to antifungal drugs. Infect Dis Clin. 2021;35(2):279-311.
Perlin DS, Rautemaa-Richardson R, Alastruey-Izquierdo A. The global problem of antifungal resistance: prevalence, mechanisms, and management. Lancet Infect Dis. 2017;17(12):e383-e392.
Fu YS, Chen TH, Weng LB, Huang LY, Lai D, Weng CF. Pharmacological properties and underlying mechanisms of curcumin and prospects in medicinal potential. Biomed Pharmacother. 2021;141:111888.
Singh A, Dasgupta S, Bhattacharya A, Mukherjee G, Chaudhury K. Therapeutic potential of curcumin in endometrial disorders: current status and future perspectives. Drug Discov Today. 2022;27(3):900-911.
Li LM, Zhang XM, Pi C, et al. Review of curcumin physicochemical targeting delivery system. Int J Nanomed. 2020;15:9799-9821.
Saifi B, Haftcheshmeh SM, Feligioni M, et al. An overview of the therapeutic effects of curcumin in reproductive disorders with a focus on the antiinflammatory and immunomodulatory activities. Phytother Res. 2022;36(2):808-823.
Peng Y, Ao MY, Dong BH, et al. Anti-inflammatory effects of curcumin in the inflammatory diseases: status, limitations and countermeasures. Drug Des Dev Ther. 2021;15:4503-4525.
Rajasekar V, Darne P, Prabhune A, et al. A curcumin-sophorolipid nano complex inhibits Candida albicans filamentation and biofilm development. Colloids Surf B Biointerfaces. 2021;200:111617.
Sanidad KZ, Sukamtoh E, Xiao H, McClements DJ, Zhang GD. Curcumin: recent advances in the development of strategies to improve oral bioavailability. Annu Rev Food Sci Technol. 2019;10:597-617.
Cao H, Duan LX, Zhang Y, Cao J, Zhang K. Current hydrogel advances in physicochemical and biological response-driven biomedical application diversity. Signal Transduct Targeted Ther. 2021;6(1):426.
Bhattaccharjee S, Beck-Broichsitter M, Banga AK. In situ gel formation in microporated skin for enhanced topical delivery of niacinamide. Pharmaceutics. 2020;12(5):472.
D’Amora U, Ronca A, Raucci MG, et al. In situ Sol-gel synthesis of hyaluronan derivatives bio-nanocomposite hydrogels. Regen Biomater. 2019;6(5):249-258.
Gutierrez AM, Frazar EM, X Klaus MV, Paul P, Hilt JZ. Hydrogels and hydrogel nanocomposites: enhancing healthcare through human and environmental treatment. Adv Healthcare Mater. 2022;11(7):e2101820.
Beard MC, Cobb LH, Grant CS, et al. Autoclaving of Poloxamer 407 hydrogel and its use as a drug delivery vehicle. J Biomed Mater Res B Appl Biomater. 2021;109(3):338-347.
Monin L, Whettlock EM, Male V. Immune responses in the human female reproductive tract. Immunology. 2020;160(2):106-115.
Chen J, Zhang JX, Chen T, et al. Xiaojianzhong decoction attenuates gastric mucosal injury by activating the p62/Keap1/Nrf2 signaling pathway to inhibit ferroptosis. Biomed Pharmacother. 2022;155:113631.
Breugelmans T, Oosterlinck B, Arras W, et al. The role of mucins in gastrointestinal barrier function during health and disease. Lancet Gastroenterol Hepatol. 2022;7(5):455-471.
Pitiot A, Ferreira M, Parent C, et al. Mucosal administration of anti-bacterial antibodies provide long-term cross-protection against Pseudomonas aeruginosa respiratory infection. Mucosal Immunol. 2023;16(3):312-325.
Kováčik A, Kopečná M, Vávrová K. Permeation enhancers in transdermal drug delivery: benefits and limitations. Expet Opin Drug Deliv. 2020;17(2):145-155.
Yadav DK, Kumar S, Choi EH, Kim MH. Electric-field-induced electroporation and permeation of reactive oxygen species across a skin membrane. J Biomol Struct Dyn. 2021;39(4):1343-1353.
Zhang H, Zhang Y, Liu CY, et al. Effect of different electrical parameters on rectum mucous membrane delivery of Gardenia jasminoides ellis extract using eletroporation. J Chin J Hospit Pharm. 2009;29(14):1181-1183.
Mohammad EA, Elshemey WM, Elsayed AA, Abd-Elghany AA. Electroporation parameters for successful transdermal delivery of insulin. Am J Therapeut. 2016;23(6):e1560-e1567.
de Melo J, Blackshaw S. In vivo electroporation of developing mouse retina. Methods Mol Biol. 2018;1715:101-111.
Fang JR, Xu JR, Xiang YT, et al. Accurate and efficient intracellular delivery biosensing system by nanostrawed electroporation array. Biosens Bioelectron. 2021;194:113583.
Aldon Y, Kratochvil S, Shattock RJ, McKay PF. Chemokine-adjuvanted plasmid DNA induces homing of antigen-specific and non-antigen-specific B and T cells to the intestinal and genital mucosae. J Immunol. 2020;204(4):903-913.
Li Q, Zhang YZ, Hu JL, et al. The improved brain-targeted drug delivery of edaravone temperature-sensitive gels by ultrasound for γ-ray radiation-induced brain injury. Pharmaceutics. 2022;14(11):2281.
Kotnik T, Rems L, Tarek M, Miklavčič D. Membrane electroporation and electropermeabilization: mechanisms and models. Annu Rev Biophys. 2019;48:63-91.
Choi SE, Khoo H, Hur SC. Recent advances in microscale electroporation. Chem Rev. 2022;122(13):11247-11286.
Pliquett UF, Martin GT, Weaver JC. Kinetics of the temperature rise within human stratum corneum during electroporation and pulsed high-voltage iontophoresis. Bioelectrochemistry. 2002;57(1):65-72.
Gurunian A, Dean DA. Modeling and simulation of current-clamp electroporation. Bioelectrochemistry. 2022;147:108162.
Bernelin-Cottet C, Urien C, McCaffrey J, et al. Electroporation of a nanoparticle-associated DNA vaccine induces higher inflammation and immunity compared to its delivery with microneedle patches in pigs. J Contr Release. 2019;308:14-28.
Zan J, Jiang GQ, Lin Y, Tan FP, Ding FX. Transdermal delivery of piroxicam by surfactant mediated electroporation. Tsinghua Sci Technol. 2005;10(5):542-547.
Guo XY, Jing TT, Li XJ, et al. Effects of boric acid gel on vaginal Candida albicans infections and the local immune system in mice. Front Immunol. 2022;13:950215.
Ourani-Pourdashti S, Mirzaei E, Heidari R, Ashrafi H, Azadi A. Preparation and evaluation of niosomal chitosan-based in situ gel formulation for direct nose-to-brain methotrexate delivery. Int J Biol Macromol. 2022;213:1115-1126.
Abdeltawab H, Svirskis D, Sharma M. Formulation strategies to modulate drug release from poloxamer based in situ gelling systems. Expet Opin Drug Deliv. 2020;17(4):495-509.
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