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Article | Open Access

Species delimitation in Pleurotus eryngii species-complex inferred from ITS and EF-1α gene sequences

Stefania M. Mang( ), Giovanni Figliuolo
Dipartimento di Biologia, Viale dell’Ateneo Lucano 10, Università degli Studi della Basilicata, Potenza 85100, Italy
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Abstract

Taxon delimitation in Pleurotus eryngii species-complex was investigated using an independent loci-based approach. A collection of 47 Pleurotus strains was analyzed through nucleotide sequences of the internal transcribed spacer region (ITS1, 5.8S and ITS2) and M13 minisatellite fragments. A sub-sample made of 12 clones representative of the ITS phylogenetic clusters was sequenced to elucidate variations in the elongation factor 1 alpha gene (EF1-α). A unique clade made of three evolutionary units, i.e. Chinese nebrodensis, Sicilian nebrodensis and eryngii-ferulae from the Mediterranean region, was revealed. The evolutionary genetic divergences were highly correlated (r = 0.86) for both genes. The average number of base substitutions per site was higher for EF1-α gene (0.013) than for ITS (0.001). M13 minisatellite polymorphism marked a clear association between strains and geographic origin but its resolution in taxa delimitation was low. Data from ITS and EF1-α genes supported the hypothesis that the species-complex is a unique gene-pool. Within the species-complex, the nebrodensis type is characterized by a level of nucleotide substitutions lower than as expected for its ranking at a species level.

Keywords

taxonomyITSelongation factor 1-alphaM13 markerPleurotus

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Mycology
Volume 1 Issue 4,
December 2010
Pages 269-280
DOI: 10.1080/21501203.2010.536171
Cite this article:
Mang SM, Figliuolo G. Species delimitation in Pleurotus eryngii species-complex inferred from ITS and EF-1α gene sequences. Mycology, 2010, 1(4): 269-280. https://doi.org/10.1080/21501203.2010.536171

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Received: 05 July 2010
Accepted: 27 October 2010
Published: 10 December 2010
© 2010 Mycological Society of China
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