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Article | Open Access

Induction of sporulation in plant pathogenic fungi

Yuan-Ying Sua, Ya-Lin Qib, Lei Caia( )
State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, P.R. China
College of Life Sciences, Capital Normal University, Beijing 100048, P.R. China
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Abstract

Spore morphologies are a major character in fungal taxonomy, although many isolates are not able to sporulate on common artificial media. This article reviews the effect of nutrition, host tissue, and light on fungal sporulation in artificial media. A trial experiment using 42 strains that failed to sporulate on potato dextrose agar (PDA) and half-strength PDA after 3 months is reported. Five strategies (1/10-strength PDA, CaCO3 water agar, pine needle medium, mulberry agar, and near-ultraviolet light irradiation) were applied to induce these strains to sporulate, with an overall success rate of 62%. Pine needle medium was the most successful method, which induced sporulation of 40% of recalcitrant strains.

Keywords

taxonomyconidiapine needle mediumsterile

References

 

Aragaki M. 1961. Radiation and temperature interaction on the sporulation of Alternaria tomato. Phytopathology. 51:803–805.

Google Scholar
 

Betina V. 1995. Photoinduced conidiation in Trichoderma viride. Folia Microbiol. 40(3):219–224.

Crossref Google Scholar
 

Booth C. 1971. Fungal culture media. Methods Microbiol. 4:49–94.

Crossref Google Scholar
 

Braun U, Crous PW, Groenewald JZ, Scheuer C. 2011. Pseudovirgaria, a fungicolous hyphomycete genus. IMA Fungus. 2(1):65–69.

Crossref Google Scholar
 

Cai L, Hyde KD, Taylor PWJ, Weir BS, Waller J, Abang MM, Zhang JZ, Yang YL, Phoulivong S, Liu ZY, et al. 2009. A polyphasic approach for studying Colletotrichum. Fungal Divers. 39:183–204.

Google Scholar
 

Charlton K. 1953. The sporulation of Alternaria solani in culture. Trans Br Mycol Soc. 36(4):349–355.

Crossref Google Scholar
 

Corrochano LM. 2007. Fungal photoreceptors: sensory molecules for fungal development and behaviour. Photochem Photobiol Sci. 6(7):725–736.

Crossref Google Scholar
 

Crous P, Slippers B, Wingfield M, Rheeder J, Marasas W, Philips A, Alves A, Burgess T, Barber P, Groenewald J. 2006. Phylogenetic lineages in the Botryosphaeriaceae. Studies Mycol. 55:235–253.

Crossref Google Scholar
 

Dahlberg KR, Etten J. 1982. Physiology and biochemistry of fungal sporulation. Annu Rev Phytopathol. 20(1):281–301.

Crossref Google Scholar
 

Engelkes C, Nuclo R, Fravel D. 1997. Effect of carbon, nitrogen, and C: N ratio on growth, sporulation, and biocontrol efficacy of Talaromyces flavus. Phytopathology. 87(5):500–505.

Crossref Google Scholar
 

Fisher N, Burgess L, Toussoun T, Nelson P. 1982. Carnation leaves as a substrate and for preserving cultures of Fusarium species. Phytopathology. 72(1):151–153.

Crossref Google Scholar
 

Fisher PJ, Petrini O, Petrini LE, Sutton RC. 1994. Fungal endophytes from the leaves and twigs of Quercus ilex L. from England, Majorca and Switzerland. New Phytol. 127(1):133–137.

Crossref Google Scholar
 

Froehlich AC, Liu Y, Loros JJ, Dunlap JC. 2002. White Collar-1, a circadian blue light photoreceptor, binding to the frequency promoter. Science. 297(5582):815–819.

Crossref Google Scholar
 

Furukawa T, Kishi K. 2002. Production of perithecia of various ascomycotina on water agar medium emended with leaf pieces. J Phytopathol. 150(11–12):625–628.

Crossref Google Scholar
 

Gao L, Sun MH, Liu XZ, Che YS. 2007. Effects of carbon concentration and carbon to nitrogen ratio on the growth and sporulation of several biocontrol fungi. Mycol Res. 111(1):87–92.

Crossref Google Scholar
 

Glienke C, Pereira OL, Stringari D, Fabris J, Kava-Cordeiro V, Galli-Terasawa L, Cunnington J, Shivas RG, Groenewald JZ, Crous PW. 2011. Endophytic and pathogenic Phyllosticta species, with reference to those associated with Citrus Black Spot. Persoonia. 26:47–56.

Crossref Google Scholar
 

Guo L, Hyde K, Liew E. 1998. A method to promote sporulation in palm endophytic fungi. Fungal Divers. 1:109–113.

Google Scholar
 

He Q, Cheng P, Yang Y, Wang L, Gardner KH, Liu Y. 2002. White collar-1, a DNA binding transcription factor and a light sensor. Science. 297(5582):840–843.

Crossref Google Scholar
 

Hernandez-Rodriguez Y, Hastings S, Momany M. 2012. The septin AspB in Aspergillus nidulans forms bars and filaments and plays roles in growth emergence and conidiation. Eukaryot Cell. 11(3):311–323.

Crossref Google Scholar
 

Hyde K, Abd-Elsalam K, Cai L. 2010. Morphology: still essential in a molecular world. Mycotaxon. 114(13):439–451.

Crossref Google Scholar
 

Idnurm A, Rodriguez-Romero J, Corrochano LM, Sanz C, Iturriaga EA, Eslava AP, Heitman J. 2006. The Phycomyces madA gene encodes a blue-light photoreceptor for phototropism and other light responses. Proc Natl Acad Sci USA. 103(12):4546–4551.

Crossref Google Scholar
 

Idnurm A, Verma S, Corrochano LM. 2010. A glimpse into the basis of vision in the kingdom Mycota. Fungal Genet Biol. 47(11):881–892.

Crossref Google Scholar
 

Kirk MP, Cannon PF, Minter DW, Stalpers JA. 2008. Dictionary of the fungi. 10th ed. Wallingford (CT): CABI. p. 653.

 

Larmour R, Marchant R. 1977. The induction of conidiation in Fusarium culmorum grown in continuous culture. J Gen Microbiol. 99(1):49–58.

Crossref Google Scholar
 

Leach CM. 1962. Sporulation of diverse species of fungi under near-ultraviolet radiation. Can J Bot. 40(1):151–161.

Crossref Google Scholar
 

Leaver FW, Leal J, Brewer CR. 1947. Nutritional studies on Pyricularia oryzae. J Bacteriol. 54:401–408.

Crossref Google Scholar
 

Li W, Zhou J, Guo SY, Guo LD. 2007. Endophytic fungi associated with lichens in Baihua mountain of Beijing, China. Fungal Divers. 25:69–80.

Google Scholar
 

Liu AR, Chen SC, Wu SY, Xu T, Guo LD, Jeewon R, Wei JG. 2010. Cultural studies coupled with DNA based sequence analyses and its implication on pigmentation as a phylogenetic marker in Pestalotiopsis taxonomy. Mol Phylogenet Evol. 57(2):528–535.

Crossref Google Scholar
 

Liu F, Hyde KD, Cai L. 2011. Neotypification of Colletotrichum coccodes, the causal agent of potato black dot disease and tomato anthracnose. Mycology. 2(4):248–254.

Crossref Google Scholar
 

Marsh PB, Taylor EE, Bassler LM. 1959. A guide to the literature of certain effects of light on fungi. Plant Dis Rep Suppl. 261:251–312.

Google Scholar
 

Masangkay RF, Paulitz TC, Hallett SG, Watson AK. 2000. Characterization of sporulation of Alternaria alternata f. sp. sphenocleae. Biocontrol Sci Technol. 10(4):385–397.

Crossref Google Scholar
 

Miller OK. 1967. The role of light in the fruiting of Panus fragilis. Can J Bot. 45(11):1939–1943.

Crossref Google Scholar
 

Misawa T. 1965. Nutritional factors for phytopathogenic fungi on culture media. Ann Phytopathol SOC Jpn. 31:27–34.

Crossref Google Scholar
 

Nirenberg HI. 1976. Untersuchungen über die morphologische und iologische Differenzierung in der Fusarium Sektion Liseola. Mitt Biol Bundesanst Land- Forstwirtsch (Berlin–Dahlem). 169:1–117.

Google Scholar
 

Okane I, Nakagiri A, Ito T. 2001. Identity of Guignardia sp. inhabiting ericaceous plants. Botany. 79(1):101–109.

Crossref Google Scholar
 

Onesirosan PT. 1978. Factors affecting sporulation by Phomopsis phaeolorum. Mycopathol. 64:23–27.

Crossref Google Scholar
 

Özcan S, Dover J, Rosenwald AG, Wölfl S, Johnston M. 1996. Two glucose transporters in S. cerevisiae are glucose sensors that generate a signal for induction of gene expression. Proc Natl Acad Sci USA. 93(22):12428–12432.

Crossref Google Scholar
 

Paul P, Munkvold G. 2005. Influence of temperature and relative humidity on sporulation of Cercospora zeae-maydis and expansion of gray leaf spot lesions on maize leaves. Plant Dis. 89(6):624–630.

Crossref Google Scholar
 

Photita W, Lumyong S, Lumyong P, Hyde KD. 2001. Endophytic fungi of wild banana (Musa acuminata) at DoiSuthep Pui National Park, Thailand. Mycol Res. 105(12):1508–1513.

Crossref Google Scholar
 

Prasad B, Dutt BL, Nagaich BB. 1973. Inducing sporulation in Alternaria solani. I. Effect of water treatment. Mycopathol Mycol Appl. 49(2–3):141–146.

Crossref Google Scholar
 

Rakoczy L. 1963. Influence of monochromatic light on the fructification of Physarum nudum. Acta Soc Bot Pol. 11:559–562.

Google Scholar
 

Rakoczy L. 1965. Action spectrum in sporulation of slime mould, Physarum nudum. Acta Soc Bot Pol. 34:97–112.

Crossref Google Scholar
 

Rands RD. 1917. The production of spores of Alternaria solani in pure culture. Phytopathology. 7:316–317.

Google Scholar
 

Riddell RW. 1950. Permanent stained mycological preparations obtained by slide culture. Mycologia. 42(2):265–270.

Crossref Google Scholar
 

Rodriguez-Romero J, Hedtke M, Kastner C, Muller S, Fischer R. 2010. Fungi, hidden in soil or up in the air: light makes a difference. Annu Rev Microbiol. 64:585–610.

Crossref Google Scholar
 

Roncal T, Ugalde U. 2003. Conidiation induction in Penicillium. Res Microbiol. 154(8):539–546.

Crossref Google Scholar
 

Rotem J. 1994. The genus Alternaria: biology, epidemiology and pathogenicity. Saint Paul (MN): APS Press.

 

Ruger-Herreros C, Rodríguez-Romero J, Fernández-Barranco R, Olmedo M, Fischer R, Corrochano LM, Canovas D. 2011. Regulation of conidiation by light in Aspergillus nidulans. Genetics. 188(4):809–897.

Crossref Google Scholar
 

Sanchez-Arregui A, Perez-Martinez AS, Herrera-Estrella A. 2012. Proteomic analysis of Trichoderma atroviride reveals independent roles for transcription factors BLR-1 and BLR-2 in light and darkness. Eukaryot Cell. 11(1):30–41.

Crossref Google Scholar
 

Sangeetha CG, Rawal RD. 2008. Nutritional studies of Colletotrichum gloeosporioides (Penz.) Penz. and Sacc. the incitant of mango anthracnose. World J Agri Sci. 4(1):717–720.

Google Scholar
 

Schafmeier T, Diernfellner ACR. 2011. Light input and processing in the circadian clock of Neurospora. FEBS Lett. 585(10):1467–1473.

Crossref Google Scholar
 

Shahin EA, Shepard JF. 1979. An efficient technique for inducing profuse sporulation of Alternaria species. Phytopathology. 69:618–620.

Crossref Google Scholar
 

Singh B. 1967. Inducing sporulation in different strains of Alternaria solani. Mycopath Mycol Appl. 32(2):163–171.

Crossref Google Scholar
 

Smith IE, Berry DR. 1974. An introduction to biochemistry of fungal development. London (UK): Academic Press. p. 326.
 

Starostzik C, Marwan W. 1995. A photoreceptor with characteristics of phytochrome triggers sporulation in the true slime mould Physarum polycephalum. FEBS Lett. 370 (1–2):146–148.

Crossref Google Scholar
 

Su YY, Guo LD, Hyde KD. 2010. Response of endophytic fungi of Stipa grandis to experimental plant function group removal in Inner Mongolia steppe, China. Fungal Divers. 43:93–101.

Crossref Google Scholar
 

Su YY, Noireung P, Liu F, Hyde KD, Moslem MA, Bahkali AH, Abd-Elsalam KA, Cai L. 2011. Epitypification of Colletotrichum musae, the causative agent of banana anthracnose. Mycoscience. 52(6):376–382.

Crossref Google Scholar
 

Sun X, Guo LD. 2012. Endophytic fungal diversity: review of traditional and molecular techniques. Mycology. 3(1):65–76.

Crossref Google Scholar
 

Sun X, Yu L, Lan N, Wei S, Yu Y, Zhang H, Zhang X, Li S. 2012. Analysis of the role of transcription factor VAD-5 in conidiation of Neurospora crassa. Fungal Genet Biol. 49(5):379–387.

Crossref Google Scholar
 

Suryanarayanan TS, Vijaykrishna D. 2001. Fungal endophytes of aerial roots of Ficus benghalensis. Fungal Divers. 8:155–161.

Google Scholar
 

Suzaki K. 2011. Improved method to induce sporulation of Colletotrichum gloeosporioides, causal fungus of grape ripe rot. J Gen Plant Pathol. 77(2):81–84.

Crossref Google Scholar
 

Sánchez Márquez S, Bills GF, Zabalgogeazcoa I. 2008. Diversity and structure of the fungal endophytic assemblages from two sympatric coastal grasses. Fungal Divers. 33:87–100.

Google Scholar
 

Tejesvi MV, Kajula M, Mattila S, Pirttilä AM. 2011. Bioactivity and genetic diversity of endophytic fungi in Rhododendron tomentosum Harmaja. Fungal Divers. 47:97–107.

Crossref Google Scholar
 

Timberlake WE. 1980. Developmental gene regulation in Aspergillus nidulans. Dev Biol. 78(2):497–510.

Crossref Google Scholar
 

Timnick MB, Lilly VG, Barnett HL. 1951. The effect of nutrition on the sporulation of Melanconium fuligineum in culture. Mycologia. 43(6):625–634.

Crossref Google Scholar
 
Tomiyama K, Sakai R, Takakuwa M. 1962. Physiology of phytopathogenic fungi. In: Asuyama H, Mukoo H, Suzuki N, editors. Laboratory guide for plant pathologists. Tokyo (Japan): Japan Plant Protection Association. p. 335–380.
 

Tzima AK, Paplomatas EJ, Tsitsigiannis DI, Kang S. 2012. The G protein beta subunit controls virulence and multiple growth- and development-related traits in Verticillium dahliae. Fungal Genet Biol. 49(4):271–283.

Crossref Google Scholar
 

Ugalde UO, Pitt D. 1986. Calcium uptake kinetics in relation to conidiation in submerged cultures of Penicillium cyclopium. Trans Br Mycol Soc. 87:199–203.

Crossref Google Scholar
 

Ugalde UO, Virto MD, Pitt D. 1990. Calcium binding and induction of conidiation in protoplasts of Penicillium cyclopium. Antonie Leeuwenhoek. 57(1):43–49.

Crossref Google Scholar
 

van der Aa HA. 1973. Studies in Phyllosticta I. Stud Mycol. 5:1–110.
Google Scholar
 

Vakalounakis DJ, Christias C. 1981. Sporulation in Alternaria cichorii is controlled by a blue and near ultraviolet reversible photoreaction. Can J Bot. 59(5):626–628.

Crossref Google Scholar
 

Wei JG, Xu T, Guo LD. 2006. Morphological stability and taxonomical significance of the genus Pestalotiopsis. J Laiyang Agric College. 23:280–284.

Google Scholar
 

Wright BE. 1979. Causality in biological systems. Trends Biochem Sci. 4:110–111.

Crossref Google Scholar
 

Wulandari N, To-Anun C, Hyde K, Duong L, de Gruyter J, Meffert J, Groenewald J, Crous P. 2009. Phyllosticta citriasiana sp. nov., the cause of Citrus tan spot of Citrus maxima in Asia. Fungal Divers. 34:23–39.

Google Scholar
 

Xu LL, Li F, Xie HY, Liu XZ. 2009. A novel method for promoting conidial production by a nematophagous fungus, Pochonia chlamydosporia AS6.8. World J Micro Biotechnol. 25(11):1989–1994.

Crossref Google Scholar
 

Xu JW, Zhao W, Xu YN, Zhong JJ. 2012. Isolation and analysis of differentially expressed genes during asexual sporulation in liquid static culture of Ganoderma lucidum by suppression subtractive hybridization. Mol Biol Rep. 39(4):3603–3610.

Crossref Google Scholar
 

Yamaguchi H. 1974. Mycelial development and chemical alteration of Candida albicans from biotin insufficiency. Sabouraudia. 12(3):320–328.

Crossref Google Scholar
 

Yazdany S, Lashkari K. 1975. Effect of pH on sporulation of Bacillus stearothermophilus. Appl Microbiol. 30(1): 1–3.

Crossref Google Scholar
 

Yoshida S, Shirata A. 2000. Biotin induces sporulation of mulberry anthracnose fungus, Colletotrichum dematium. J Genl Plant Pathol. 66(2):117–122.

Crossref Google Scholar
 

Zhou G, Wang J, Qiu L, Feng MG. 2012. A Group Ⅲ histidine kinase (mhk1) upstream of high-osmolarity glycerol pathway regulates sporulation, multi-stress tolerance and virulence of Metarhizium robertsii, a fungal entomopathogen. Environ Microbiol. 14(3):817–829.
Crossref Google Scholar
Mycology
Volume 3 Issue 3,
September 2012
Pages 195-200
DOI: 10.1080/21501203.2012.719042
Cite this article:
Su Y-Y, Qi Y-L, Cai L. Induction of sporulation in plant pathogenic fungi. Mycology, 2012, 3(3): 195-200. https://doi.org/10.1080/21501203.2012.719042

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Received: 31 July 2012
Accepted: 31 July 2012
Published: 23 August 2012
© 2012 Mycological Society of China
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