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Article | Open Access

Antagonistic effects of Saccharomyces cerevisiae on the growth of Aspergillus flavus and Aspergillus parasiticus at varying temperatures

Karen Personsa, Joseph M. Rainesa, Jose M. Rodrigueza,b( )
Mississippi State Chemical Laboratory, P.O. Box CR, MS 39762, USA
Department of Biochemistry, Molecular Biology, Entomology and Plant Pathology, Mississippi State University, MS 39762, USA
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Abstract

A pour-plate dilution method was employed to assess the antagonistic effects of Saccharomyces cerevisiae on the growth of both Aspergillus flavus and Aspergillus parasiticus at 22℃, 25℃, and 32℃. Dilutions of S. cerevisiae were pour plated, and upon solidification, the plates were inoculated with a lawn of either A. flavus or A. parasiticus. Results of this experiment suggest that higher concentrations of S. cerevisiae are effective in inhibiting the growth of both Aspergillus species investigated at all temperatures, but its effects are most pronounced at 22℃. Broader implications of this study include the possibility that S. cerevisiae may be used as a biocontrol agent to protect agricultural products commonly consumed by humans from the toxigenic metabolites produced by A. flavus and A. parasiticus.

Keywords

inhibitionaflatoxinbiocontrolbaker's yeast

References

 

Bjornberg A, Schnurer J. 1993. Inhibition of the growth of grain-storage molds in vitro by the yeast Pichia anomala (Hansen) Kurtzman. Can J Mirobiol. 39:623–628.
Crossref Google Scholar
 

Bleve G, Grieco F, Cozzi G, Logrieco A, Visconti A. 2006. Isolation of epiphytic yeasts with potential for biocontrol of Aspergillus carbonarius and A. Niger on grape. Int J Food Microbiol. 108:204–209.
Crossref Google Scholar
 

Craig EA, Jacobsen K. 1984. Mutations of the heat inducible 70 kilodalton genes of yeast confer temperature sensitive growth. Cell. 38:841–849.
Crossref Google Scholar
 

Cubaiu L, Abbas A, Dobson ADW, Budroni M, Migheli Q. 2009. Effect of antagonistic Saccharomyces cerevisiae on the expression of the PKS gene in ochratoxin A producing Aspergillus spp. J Plant Pathol. 91:56
Google Scholar
 

Cvetnic Z, Pepeljnjak S. 2007. Interaction between certain moulds and aflatoxin B1 producer Aspergillus flavus NRRL 3251. Arh Hig Rada. Tokiskol. 58:429–434.
Crossref Google Scholar
 

Dimakopoulou M, Tjamos SE, Antoniou PP, Pietri A, Battilani P, Avramidis N, Markakis EA, Tjamos EC. 2008. Phllyosphere grapevine yeast Aureobasidium pullulans reduces Aspergillis carbonarius (sour rot) incidence in wine-producing vineyards in Greece. Biol Control. 46:158–165.
Crossref Google Scholar
 

Haskard CA, El-Nezami HS, Kankaanpaa PE, Salminen S, Ahokas JT. 2001. Surface binding of aflatoxin B1 by lactic acid bacteria. Appl Env Microbiol. 67:3086–3091.
Crossref Google Scholar
 

Joannis-Cassan C, Tozlovanu M, Hadjeba-Medjdoub K, Ballet N, Pfohl-Leszkowicz A. 2011. Binding of zearalenone, aflatoxin B1 and ochratoxin A by yeast-based products: a method for quantification of adsorption performance. J Food Protect. 74:1175–1185.
Crossref Google Scholar
 

La Penna M, Nesci A, Etcheverry M. 2004. In vitro studies on the potential for biological control on Aspergillus section Flavi by Kluyveromyces spp. Letters in Appl Microbiol. 38:257–264.
Crossref Google Scholar
 

Liu S, Tsao M. 2009. Biocontrol of dairy moulds by antagonistic dairy yeast Debaryomyces hansenii in yoghurt and cheese at elevated temperatures. Food Contr. 20:852–855.
Crossref Google Scholar
 

Lopez-Malo A, Alzamora SM, Palou E. 2005. Aspergillus flavus growth in the presence of chemical preservatives and naturally occurring antimicrobial compounds. Int J Food Microbiol. 99:119–128.
Crossref Google Scholar
 

Marquina D, Santos A, Peinado J. 2002. Biology of killer yeasts. Int Microbiol. 5:65–71.
Crossref Google Scholar
 

Masoud W, Jakobsen M. 2005. Influence of volatile compounds produced by yeasts predominant during processing of Coffea arabica in East Africa on growth and ochratoxin A (OTA) production by Aspergillus ochraceus. Yeast. 22:1133–1142.
Crossref Google Scholar
 

Masoud W, Kaltoft CH. 2006. The effects of yeast involved in the fermentation of Coffea Arabica in East Africa on growth and ochratoxin A (OTA) production by Aspergillus ochraceus. Int J Food Microbiol. 106:229–234.
Crossref Google Scholar
 

Olarte RA, Horn BW, Monacell JT, Stone EA, Carbone I. 2010. Insights into sexual reproduction in Aspergillus flavus from variation in experimental crosses and natural populations. Phytopathol. 100:S92
Google Scholar
 

Paster N, Droby S, Chalutz E, Menasherov M, Nitzan R, Wilson CL. 1993. Evaluation of the potential of the yeast Pichia guilliermondii as a biocontrol agent against Aspergillus flavus and fungi of stored soya beans. Mycol Res. 97:1201–1206.
Crossref Google Scholar
 

Petersson S, Hansen MW, Axberg K, Hult K, Schniirer J. 1998. Ochratoxin A accumulation in cultures of Penicillium verrucosum with the antagonistic yeast Pichia anomala and Saccharomyces cerevisiae. Mycol Res. 102:1003–1008.
Crossref Google Scholar
 

Petersson S, Jonsson N, Schnurer J. 1999. Pichia anomala as a biocontrol agent during storage of high-moisture feed grain under airtight conditions. Postharvest Biol Tech. 15:175–184.
Crossref Google Scholar
 

Pitt JI, Hocking AD. 2006. Mycotoxins in Australia: biocontrol of aflatoxin in peanuts. Mycopathol. 162:233–243.
Crossref Google Scholar
 

Prado G, Madeira JEG, MoralsVA D, Oliveira MS, Souza RA, Peluzio JM, Godoy IJ, Silva JFM, Pimento RS. 2011. Reduction of aflatoxin B1 in stored peanuts (Arachis hypogaea L.) using Saccharomyces cerevisiae. J Food Protect. 74:1003–1006.
Crossref Google Scholar
 

Rasooli I, Abyaneh MR. 2004. Inhibitory effects of thyme oils on growth and aflatoxin production by Aspergillus parasiticus. Food Contr. 15:479–483.
Crossref Google Scholar
 

Robens J, Cardwell K. 2003. The costs of mycotoxin management to the USA:management of aflatoxins in the United States. J Toxicol. 22:139–152.
Crossref Google Scholar
 

Schindler AF, Palmer JG, Eisenberg WV. 1967. Aflatoxin production by Aspergillus flavus as related to various temperatures. Appl Microbiol. 15:1006–1009.
Crossref Google Scholar
 
Serna JG, Patino Alvarez B, Gonzalez-Jaen MT, Vazquez Estevez C. 2009. Biocontrol of Aspergillus ochraceus by yeasts. In: Mendez-Vilas A, editor. Current research topics in applied microbiology and microbial biotechnology. Hackensack, NJ: World Scientific; p. 368–372.
Crossref
 

Somai BM, Belewa V. 2011. Aqueous extract of Tulbaghia violacea inhibit germination of Aspergillus flavus and Aspergillus parasiticus conidia. J Food Protect. 74:1007–1011.
Crossref Google Scholar
 

Sood M. 2011. Effect of temperature of incubation on the growth, sporulation and secondary metabolites production of Aspergillus umbrosus. J Phytol. 3:35–37.
Google Scholar
 

Sorger PK, Pelham HRB. 1988. Yeast heat shock factor is an essential DNA-binding protein that exhibits temperature-dependent phosphorylation. Cell. 54:855–864.
Crossref Google Scholar
 

Sprague GF Jr, Winans SC. 2006. Eukaryotes learn how to count: quorum sensing by yeast. Genes Dev. 20:1045–1049.
Crossref Google Scholar
 

Varga J, Frisvad JC, Samson RA. 2011. Two new aflatoxin producing species, and an overview of Aspergillus section Flavi. Stud Mycol. 69:57–80.
Crossref Google Scholar
 

Velmourougane K, Bhat R, Gopinandhan TN, Panneerselvam P. 2011. Management of Aspergillus ochraceus and ochratoxin-A contamination in coffee during on-farm processing through commercial yeast inoculation. Biol Control. 57:215–221.
Crossref Google Scholar
 

Yu MC, Yuan J. 2004. Environmental factors and risk for hepatocellular carcinoma. Gastroenterol. 127:S72–S78.
Crossref Google Scholar
 

Zain ME, Ratak AA, El-Sheikh HH, Soliman HG, Khalil AM. 2009. Influence of growth medium on diagnostic characters of Aspergillus and Penicillium species. Afr J Microbial Res. 3:280–286.
Google Scholar
Mycology
Volume 4 Issue 1,
March 2013
Pages 38-43
DOI: 10.1080/21501203.2012.754796
Cite this article:
Persons K, Raines JM, Rodriguez JM. Antagonistic effects of Saccharomyces cerevisiae on the growth of Aspergillus flavus and Aspergillus parasiticus at varying temperatures. Mycology, 2013, 4(1): 38-43. https://doi.org/10.1080/21501203.2012.754796

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Received: 19 November 2012
Accepted: 28 November 2012
Published: 05 February 2013
© 2013 Mycological Society of China
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