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Article | Open Access

Antimicrobial activity of crude extracts of Phyllosticta spp.

Saowanee Wikeea,b, Phatcharapa Jaideea, Sakuntala Wongkama, Eric H.C. Mckenziec, Kevin D. Hydea,b, Ekachai Chukeatirotea,b( )
School of Science, Mae Fah Luang University, Chiang Rai 57100, Thailand
Institute of Excellence in Fungal Research, Mae Fah Luang University, Chiang Rai 57100, Thailand
Landcare Research, Private Bag 92170, Auckland Mail Centre, Auckland 1142, New Zealand
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Abstract

Four strains of Phyllosticta were selected to study their antimicrobial activities. The tested strains were identified as Phyllosticta capitalensis, P. citriasiana, and P. cordylinophila based on the internally transcribed spacer (ITS) sequences and morphologies. Initially, the fungal strains were cultured on the potato dextrose agar (PDA) and cultivated at 27℃ for 3 weeks. After incubation, the fungal metabolites were extracted using ethyl acetate. The crude extracts were then concentrated using evaporation and subsequently used in antimicrobial activity assay. Our results showed that these fungal crude extracts could inhibit the growth of Escherichia coli, Bacillus cereus, and Pseudomonas aeruginosa but were not active against Aspergillus niger.

Keywords

antimicrobial activitysecondary metabolitesGuignardia

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Mycology
Volume 4 Issue 2,
June 2013
Pages 112-117
DOI: 10.1080/21501203.2013.823892
Cite this article:
Wikee S, Jaidee P, Wongkam S, et al. Antimicrobial activity of crude extracts of Phyllosticta spp.. Mycology, 2013, 4(2): 112-117. https://doi.org/10.1080/21501203.2013.823892

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Received: 23 April 2013
Accepted: 02 July 2013
Published: 09 August 2013
© 2013 Mycological Society of China
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