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Invited Article | Open Access

Sea foam as a source of fungal inoculum for the isolation of biologically active natural products

David P. Overya,b,c, Fabrice Berruea,b, Hebelin Correaa, Novriyandi Hanifb, Kathryn Hayd, Martin Lanteigneb, Kathrine Mquiliana, Stephanie Duffya, Patricia Bolandb, Ramesh Jagannathana, Gavin S. Carra, Marieke Vansteelanda, Russell G. Kerra,b,e( )
Nautilus Biosciences Canada Inc., Duffy Research Center, 550 University Ave., Charlottetown, PEI, Canada C1A 4P3
Department of Chemistry, University of Prince Edward Island, 550 University Ave., Charlottetown, PEI, Canada C1A 4P3
Department of Pathology and Microbiology, Atlantic Veterinary College, University of Prince Edward Island, 550 University Ave., Charlottetown, PEI, Canada C1A 4P3
Department of Biology, University of Prince Edward Island, 550 University Ave., Charlottetown, PEI, Canada C1A 4P3
Department of Biomedical Sciences, Atlantic Veterinary College, University of Prince Edward Island, 550 University Ave., Charlottetown, PEI, Canada C1A 4P3
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Abstract

Due to a rate increase in the resistance of microbial pathogens to currently used antibiotics, there is a need in society for the discovery of novel antimicrobials. Historically, fungi are a proven source for antimicrobial compounds. The main goals of this study were to investigate the fungal diversity associated with sea foam collected around the coast of Prince Edward Island and the utility of this resource for the production of antimicrobial natural products. Obtained isolates were identified using ITS and nLSU rDNA sequences, fermented on four media, extracted and fractions enriched in secondary metabolites were screened for antimicrobial activity. The majority of the isolates obtained were ascomycetes, consisting of four recognized marine taxa along with other ubiquitous genera and many ‘unknown’ isolates that could not be identified to the species level using rDNA gene sequences. Secondary metabolite isolation efforts lead to the purification of the metabolites epolones A and B, pycnidione and coniothyrione from a strain of Neosetophoma samarorum; brefeldin A, leptosin J and the metabolite TMC-264 from an unknown fungus (probably representative of an Edenia sp.); and 1-hydroxy-6-methyl-8-hydroxymethylxanthone, chrysophanol and chrysophanol bianthrone from a Phaeospheria spartinae isolate. The biological activity of each of these metabolites was assessed against a panel of microbial pathogens as well as several cell lines.

Keywords

antimicrobialsfungal diversitysecondary metabolitessea foamNeosetophoma samarorumPhaeosphaeria spartinae

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Mycology
Volume 5 Issue 3,
September 2014
Pages 130-144
DOI: 10.1080/21501203.2014.931893
Cite this article:
Overy DP, Berrue F, Correa H, et al. Sea foam as a source of fungal inoculum for the isolation of biologically active natural products. Mycology, 2014, 5(3): 130-144. https://doi.org/10.1080/21501203.2014.931893

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Received: 04 March 2014
Accepted: 02 June 2014
Published: 22 July 2014
© 2014 The Author(s).

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The moral rights of the named author(s) have been asserted.
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