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Original Article | Open Access

Qip gene in Fusarium oxysporum is required for normal hyphae morphology and virulence

Lin Chenga, Jian Lingb, Liqin Lianga, Zhongqin Luob, Jie Zhanga, Bingyan Xieb( )
College of Life Science, Shanxi Normal University, Gong yuan Street No. 1, Yaodu, Linfen 041004, China
Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, South Street No. 12, Zhongguancun, Haidian, Beijing 100081, China
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Abstract

Ribonucleic acid (RNA)-silencing mechanisms exist in many eukaryotes to regulate a variety of biological processes. The known molecular components are related to Dicers, Argonautes and RNA-dependent RNA polymerases. Previous biochemical studies have also suggested that Qip, with an exonuclease domain, facilitates the conversion of duplex small interfering RNAs into single strands. In our study, the Qip gene in Fusarium oxysporum was disrupted using homologous recombination technology. The deletion of the Qip gene resulted in a decrease in colony growth rates but increased the number of branches. Additionally, the ΔQip mutant had a reduced pathogenicity in cabbage. Our results show Qip gene in F. oxysporum is required for normal hyphae morphology and virulence. The mutant will be useful for elucidating the relationship between the RNA-silencing mechanism and hyphal growth and development in F. oxysporum.

Keywords

pathogenicityFusarium oxysporumRNA-silencing mechanismQip genehomologous recombination technologyhyphal growth and development

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Mycology
Volume 6 Issue 2,
June 2015
Pages 130-137
DOI: 10.1080/21501203.2015.1027313
Cite this article:
Cheng L, Ling J, Liang L, et al. Qip gene in Fusarium oxysporum is required for normal hyphae morphology and virulence. Mycology, 2015, 6(2): 130-137. https://doi.org/10.1080/21501203.2015.1027313

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Received: 25 December 2014
Accepted: 04 March 2015
Published: 25 March 2015
© 2015 Mycological Society of China

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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