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Article | Open Access

Antimicrobial activities of endophytic fungi of the Sri Lankan aquatic plant Nymphaea nouchali and chaetoglobosin A and C, produced by the endophytic fungus Chaetomium globosum

Ranga K. Dissanayakea, Pamoda B. Ratnaweeraa,b,, David E. Williamsb, C. Dilrukshi Wijayarathnea, Ravi L. C. Wijesunderac, Raymond J. Andersenb, E. Dilip de Silvaa( )
Department of Chemistry, University of Colombo, Colombo, 03, Sri Lanka
Departments of Chemistry and Earth, Ocean and Atmospheric Sciences, University of British Columbia (UBC), Vancouver, Canada
Department of Plant Sciences, University of Colombo, Colombo, 03, Sri Lanka

Present address for Pamoda B. Ratnaweera: Department of Science and Technology, Uva Wellassa University, Passara Road, Badulla, Sri Lanka

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Abstract

Twenty distinct endophytic fungi were isolated from the surface-sterilized plant parts of Nymphaea nouchali and were identified using morphological and molecular techniques. At 300 µg/disc concentration, eight of the 20 fungal extracts exhibited antimicrobial activities against Staphylococcus aureus (ATCC 25923) and Bacillus cereus (ATCC 11778) while two within the eight showed activity against Pseudomonas aeruginosa (ATCC 9027) and Escherichia coli (ATCC 35218). Furthermore, investigation of the crude extract of Chaetomium globosum resulted in the isolation of two known cytochalasans, chaetoglobosin A and C, and their structures were elucidated and confirmed by mass and nuclear magnetic resonance (NMR) (1H, 13C, COSY, HSQC, HMBC and tROESY) spectral data. Chaetoglobosin A showed antibacterial activities against Bacillus subtilis (MIC 16 µg mL−1), Staphylococcus aureus (MIC 32 µg mL−1) and methicillin-resistant Staphylococcus aureus (MRSA, MIC 32 µg mL−1). This is the first study to report the isolation, identification and antimicrobial properties of endophytic fungi of N. nouchali in Sri Lanka.

Keywords

antimicrobialEndophytic fungiNymphaea nouchaliaquatic plantChaetomium globosumchaetoglobosin AC

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Mycology
Volume 7 Issue 1,
March 2016
Pages 1-8
DOI: 10.1080/21501203.2015.1136708
Cite this article:
Dissanayake RK, Ratnaweera PB, Williams DE, et al. Antimicrobial activities of endophytic fungi of the Sri Lankan aquatic plant Nymphaea nouchali and chaetoglobosin A and C, produced by the endophytic fungus Chaetomium globosum. Mycology, 2016, 7(1): 1-8. https://doi.org/10.1080/21501203.2015.1136708

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Received: 04 October 2015
Accepted: 16 December 2015
Published: 16 February 2016
© 2016 The Author(s).

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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