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Original Article | Open Access

Experimental design of response surface methodology used for utilisation of palm kernel cake as solid substrate for optimised production of fungal mannanase

Saroj Ahirwar,*, Hemant Soni,*, Hemant Kumar Rawat, Bhanu Pratap Prajapati, Naveen Kango( )
Department of Microbiology, Dr. Harisingh Gour University, Sagar, India

*These authors contributed equally to this work.

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Abstract

The results obtained from this work strongly indicate that the solid state fermentation (SSF) system using the palm kernel cake (PKC) as a substrate is an economical method for the production of β-mannanase at extremely low operational cost based on the fact that PKC is one of the cheap and abundant agro-waste by-products of the palm oil industry. Under initial conditions, i.e. 2 mm particle size of PKC, the moisture ratio of 1:1 of PKC:moistening agent and pH 7, Malbranchea cinnamomea NFCCI 3724 produced 109 U/gram distribution of the substrate (gds). The production of β-mannanase was optimised by the statistical approach response surface methodology (RSM) using independent variables, namely initial moisture (12.5), pH (9.0) and solka floc (100 mg). Noticeably, six fold enhancement of β-mannanase production (599 U/gds) was obtained under statistically optimised conditions. HPLC results revealed that β-mannanase is an endo-active enzyme that generated manno-oligosaccharides with a degree of polymerisation (DP) of 3 and 4. Semi-native PAGE analysis revealed that M. cinnamomea produced three isoforms of mannanase. Selective production of oligosaccharide makes M. cinnamomea β-mannanase an attractive enzyme for use in food and nutraceutical industries.

Keywords

response surface methodologyfungiAgro-wastepalm kernel cakeβ-mannanase

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Mycology
Volume 7 Issue 3,
September 2016
Pages 143-153
DOI: 10.1080/21501203.2016.1229697
Cite this article:
Ahirwar S, Soni H, Rawat HK, et al. Experimental design of response surface methodology used for utilisation of palm kernel cake as solid substrate for optimised production of fungal mannanase. Mycology, 2016, 7(3): 143-153. https://doi.org/10.1080/21501203.2016.1229697

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Received: 13 June 2016
Accepted: 24 August 2016
Published: 12 September 2016
© 2016 The Author(s).

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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