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Original Article | Open Access

Antiangiogenic, wound healing and antioxidant activity of Cladosporium cladosporioides (Endophytic Fungus) isolated from seaweed (Sargassum wightii)

Manjunath M. Hulikere, Chandrashekhar G. Joshi( ), D. Ananda, Jagadeesh Poyya, T. Nivya
Department of Biochemistry, Mangalore University, P.G.Centre, Chikka Aluvara, Kodagu, India
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Abstract

Endophytic fungi from marine seaweeds are the less studied group of organisms with vast medical applications. The aim of the present study was to evaluate antioxidant, antiangiogenic as well as wound healing potential of the endophytic fungus isolated from the seaweed Sargassum wightii. The morphological characters and the rDNA internal transcribed spacer sequence analysis (BLAST search in Gen Bank database) was used for the identification of endophytic fungus. The antioxidant potential of the ethyl acetate extract of endophytic fungus was assessed by, 1,1-diphenyl-2-picryl-hydrazyl radical scavenging method. The fungal extract was also analysed for reducing power, total phenolic and flavonoid content. Antiangiogenic activity of the fungal extract was studied in vitro by inhibition of wound healing scratch assay and in vivo by Chick chorioallantoic membrane assay. The endophytic fungus was identified as Cladosporium cladosporioides (Gen Bank ID – KT384175). The ethyl acetate extract of C. cladosporioides showed a significant antioxidant and angiosuppressive activity. The ESI-LC-MS analysis of the extract revealed the presence of wide range of secondary metabolites. Results suggest that C. cladosporioides extract could be exploited as a potential source for angiogenic modulators.

Keywords

angiogenesisantioxidant activityPolyphenolsCladosporium cladosporioidesSargassum wightiiCAM assay

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Mycology
Volume 7 Issue 4,
December 2016
Pages 203-211
DOI: 10.1080/21501203.2016.1263688
Cite this article:
Hulikere MM, Joshi CG, Ananda D, et al. Antiangiogenic, wound healing and antioxidant activity of Cladosporium cladosporioides (Endophytic Fungus) isolated from seaweed (Sargassum wightii). Mycology, 2016, 7(4): 203-211. https://doi.org/10.1080/21501203.2016.1263688

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Received: 12 September 2016
Accepted: 17 November 2016
Published: 14 December 2016
© 2016 The Author(s).

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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