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Original Article | Open Access

Molecular profiling and antioxidant as well as anti-bacterial potential of polyphenol producing endophytic fungus-Aspergillus austroafricanus CGJ-B3

Ananda Danagoudara, Chandrashekhar G Joshia( ), R. Sunil Kumara, Jagadeesha Poyyaa, T Nivyaa, Manjunath M. Hulikerea, KA Anu Appaiahb
Department of Biochemistry, Mangalore University, P.G Centre, Chikka Aluvara, Kodagu, India
Department of Microbiology and Fermentation Technology, CSIR- Central Food Technological Research Institute, Mysore, India
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Abstract

Fungal endophytes are less studied group of microorganisms with vast therapeutic potential. A polyphenol producing endophyticfungus was isolated from Zingiber officinale rhizome. The ethyl acetate extract of Aspergillus austroafricanus (EAE) was tested against five human pathogenic bacteria by disc diffusion method. Antioxidant activity of EAE was determined by DPPH, H2O2 and nitric oxide radical-scavenging methods. DNA protection from the OH radicals was tested by agarose gel electrophoresis. High-performance liquid chromatography was used to determine the total yield of polyphenols. The identity of the endophytic fungus was established as A. austroafricanus CGJ-B3 (GenBank accession No. KT780617) based on rDNA and phylogenetic analysis. EAE showed significant antioxidant, antimicrobial activity and DNA damage protection capacity. The HPLC analysis showed the presence of polyphenols such as p-coumaric acid, ferulic acid and cinnamic acid and the content was about 0.392 ± 0.08 µg/mg, 4.35 ± 0.16 µg/mg and 1.976 ± 0.11 µg/mg, respectively. A. austroafricanus CGJ-B3 isolated from Z. officinale is a promising potential pharmaceutical agent and can be used as an alternative source of polyphenols like p-coumaric acid, ferulic acid and cinnamic acid.

Keywords

polyphenolssecondary metaboliteEndophytic fungiAspergillus austroafricanus CGJ-B3Zingiber officinaleantimicrobiol activity

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Mycology
Volume 8 Issue 1,
March 2017
Pages 28-38
DOI: 10.1080/21501203.2017.1281358
Cite this article:
Danagoudar A, Joshi CG, Kumar RS, et al. Molecular profiling and antioxidant as well as anti-bacterial potential of polyphenol producing endophytic fungus-Aspergillus austroafricanus CGJ-B3. Mycology, 2017, 8(1): 28-38. https://doi.org/10.1080/21501203.2017.1281358

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Received: 17 December 2016
Accepted: 09 January 2017
Published: 30 January 2017
© 2017 The Author(s).

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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