AI Chat Paper
Note: Please note that the following content is generated by AMiner AI. SciOpen does not take any responsibility related to this content.
{{lang === 'zh_CN' ? '文章概述' : 'Summary'}}
{{lang === 'en_US' ? '中' : 'Eng'}}
Chat more with AI
Powered by AMiner. Clicking this button will take you away from SciOpen.
Home Mycology Article
Article Link
Cite
EndNote(RIS) BibTeX
Collect
Submit Manuscript
Show Outline
Outline
Show full outline
Hide outline
Outline
Show full outline
Hide outline
Article | Open Access

Isolation and screening of thermophilic and thermotolerant fungi for production of hemicellulases from heated environments

Saroj Ahirwar, Hemant Soni, Bhanu Pratap Prajapati, Naveen Kango( )
Department of Microbiology, Dr. Hari Singh Gour Vishwavidyalaya, Sagar, MP, India
Show Author Information

Abstract

Thermostable hemicellulases have potential to improve the quality of products of various industries including pulp and paper, food and feed, textile, etc. This study was aimed to isolate, screen, and identify potential xylanase and mannanase producers from heated environments. Sixty-eight thermophilic and thermotolerant fungi were isolated from various self-heated habitats based on their ability to grow at 45℃. With the aid of cultural and morphological observations, the fungi were identified and grouped into 19 fungal species. The enzyme production was evaluated by cultivating isolated fungi in submerged fermentation using wheat bran as carbon source. Screening of these isolates for hydrolysis of xylan and mannan was confirmed by Congo red plate assay method followed by assessment of xylanase and mannanase activity. Based on experimental analysis, we have found that all the isolates have exhibited xylanase activity, whereas only 22 isolates have found positive for mannanase activity. The highest xylanase and mannanase production was obtained by the cultivation of Malbranchea cinnamomea NFCCI 3724 (242 and 27 nkat/ml) followed by Melanocarpus albomyces (195 and 24 nkat/ml), Aspergillus terreus (165 and 21 nkat/ml), and Myceliophthora thermophila NFCCI 3725 (130 and 18 nkat/ml), respectively.

Keywords

enzymatic activitysubmerged fermentationxylanaseThermophilic fungimannanase

References

 

Ahirwar S, Soni H, Rawat HK, Prajapati BP, Kango N. 2016. Experimental design of response surface methodology used for utilization of palm kernel cake as solid substrate for optimised production of fungal mannanase. Mycology Int J Fungal Biol. 7:143–153.
Crossref Google Scholar
 

Berlin A, Maximenko V, Gilkes N, Saddler J. 2007. Optimization of enzyme complexes for lignocellulose hydrolysis. Biotechnol Bioeng. 97:287–296.
Crossref Google Scholar
 

Bru-Adan V, Wéry N, Moletta-Denat M, Boiron P, Delgenes JP, Godon JJ. 2009. Diversity of bacteria and fungi in aerosols during screening in a green waste composting plant. Curr Microbiol. 59:326–335.
Crossref Google Scholar
 

Chadha BS, Harmeet G, Mandeep M, Saini HS, Singh N. 2004. Phytase production by the thermophilic fungus Rhizomucor pusillus. World J Microbiol Biotechnol. 20:105–109.
Crossref Google Scholar
 

Collins T, Gerday C, Feller G. 2005. Xylanases, xylanase families and extremophilic xylanases. FEMS Microbiol Rev. 29:3–23.
Crossref Google Scholar
 
Cooney DG, Emerson R. 1964. Methods of isolation and culture. In thermophilic fungi, an account of their biology, activities and classification. San Francisco: W.H. Freeman & Company; p. 8–13.
 

Hassouni H, Smaili-Alaoui IM, Gaime-Perraud I, Augur C, Roussos S. 2006. Effect of culture media and fermentation parameters on phytase production by the thermophilic fungus Myceliophthora thermophila in solid state fermentation. Mycol Apli Int. 18:29–36.
Google Scholar
 
Johri BN, Satyanarayana T, Olsen J. 1999. Thermophilic molds in biotechnology. UK: Kluwer Academic Publishers.
Crossref
 

Juturu V, Wu JC. 2013. Insight in to microbial hemicellulases other than xylanases: a review. J Chem Technol Biotechnol. 88:353–363.
Crossref Google Scholar
 

Kango N, Agrawal SC, Jain PC. 2003. Production of xylanase by Emericella nidulans NK-62 on low-value lignocellulosic substrates. World J Microbiol Biotechnol. 19:691–694.
Crossref Google Scholar
 
Kango N., Jain P.C. (2005). Production and application of fungal xylanases. In: Rai, M.K. and Deshmukh S.K., editors. Fungi: Diversity and Biotechnology, Scientific Publishers, New Delhi. pp. 251–281
 

Krisana A, Rutchadaporn S, Jarupan G, Lily E, Sutipa T, Kanyawim K. 2005. Endo-1,4-β- xylanase from Aspergillus niger BCC14405 isolated in Thailand: purification, characterization and gene isolation. J Biochem Mol Biol. 38:17–23.
Crossref Google Scholar
 

Langarica-Fuentes A, Handley PS, Houlden A, Fox G, Robson GD. 2014. An investigation of the biodiversity of thermophilic and thermotolerant fungal species in composts using culture-based and molecular techniques. Fungal Ecol. 11:132–144.
Crossref Google Scholar
 

Lee H, Lee YM, Jang Y, Lee S, Lee H, Ahn BJ, Gyu-H K, Kim J-J. 2014. Isolation and analysis of the enzymatic properties of thermophilic fungi from compost. Mycobiology. 42:181–184.
Crossref Google Scholar
 

Lin TC, Chen C. 2004. Enhanced mannanase production by submerged culture of Aspergillus niger NCH-189 using defatted copra based media. Process Biochem. 39:1103–1109.
Crossref Google Scholar
 

Lu H, Zhang H, Shi P, Luo H, Wang Y, Yang P, Yao BA. 2013. Family 5 β-mannanase from the thermophilic fungus Thielavia arenaria XZ7 with typical thermophilic enzyme features. Appl Microbiol Biotechnol. 97:8121–8128.
Crossref Google Scholar
 

Maheshwari R, Bharadwaj G, Bhat MK. 2000. Thermophilic fungi: their physiology and enzymes. Microbiol Mol Biol Rev. 64:461–488.
Crossref Google Scholar
 

Maheshwari R, Kamalam PT, Balasubrmanyam DV. 1987. The biogeography of thermophilic fungi. Current Science. 56:151–155.
Google Scholar
 

Maijala P, Kango N, Szijarto N, Viikari L. 2012. Characterization of hemicellulases from thermophilic fungi. Anton Van Leeuwenhoek. 101:905–917.
Crossref Google Scholar
 

Miller GL. 1959. Use of dinitrosalicylic acid reagent for determination of reducing sugars. Anal Chem. 31:426–428.
Crossref Google Scholar
 
Moretti MMS, Bocchini-Martins DA, DaSilva R, Rodrigues A, Sette LD, Gomes E. 2012. Selection of thermophilic and thermotolerant fungi for the production of cellulases and xylanases under solid-state fermentation. Braz J 43(3):1062–1071.
Crossref
 

Puchart V, Vrsanska M, Svoboda P, Pohl J, Biely P. 2004. Purification and characterization of two forms of endo-β-1,4-mannanase from a thermotolerant fungus, Aspergillus fumigatus IMI 385708 (formerly T. lanuginosus IMI 158749). Biochem Biophysi Acta. 1674:239–250.
Crossref Google Scholar
 

Rajavaram RK, Bathini S, Sivadevuni G, Reddy SM. 2010. Incidence of thermophilic fungi from different substrates in Andhra Pradesh (India). Int Pharma Biosci. 1:1–6.
Google Scholar
 

Robledo A, Aguilar CN, Belmares-Cerda RE, Flores-Gallegos AC, Contreras-Esquivel JC, Montañez JC, Mussatto SI. 2015. Production of thermostable xylanase by thermophilic fungal strains isolated from maize silage. Cyta – J Food. doi:10.1080/19476337.2015.1105298
Crossref Google Scholar
 

Sadaf A, Khare SK. 2014. Production of Sporotrichum thermophile xylanase by solid state fermentation utilizing deoiled Jatropha curcas seed cake and its application in xylooligosachharide synthesis. Bioresour Technol. 153:126–130.
Crossref Google Scholar
 

Salar RK, Aneja KR. 2006. Thermophilous fungi from temperate soils of northern India. J Agric Technol. 2:49–58.
Google Scholar
 

Sebok F, Dobolyi C, Bobvos J, Szoboszlay S, Kriszt B, Magyar D. 2015. Thermophilic fungi in air samples in surroundings of compost piles of municipal, agricultural and horticultural origin. Aerobiologia. 32:255–263.
Crossref Google Scholar
 
Singh B, Satyanarayana T. 2009. Thermophilic molds in environmental management. In: Misra JK, Deshmukh SK, editor. Progress in mycological research, vol. I. Fungi from different environments. Environmental mycology. USA: Science Publishers; p. 352–375.
Crossref
 

Soni H, Kango N. 2013. Hemicellulases in lignocellulose biotechnology: recent patents. Rec Patents Biotechnol (Bentham Science Publishers). 7:207–218.
Crossref Google Scholar
 

Soni H, Rawat HK, Ahirwar S, Kango N. 2016. Screening, statistical optimized production, and application of β-mannanase from some newly isolated fungi. Eng Life Sci. doi:10.1002/elsc.201600136
Crossref Google Scholar
 

Warcup JH. 1950. The soil plate method for isolation of fungi from soil. Nature. 166:117–118.
Crossref Google Scholar
 

Yang H, Shi P, Lu H, Wang H, Luo H, Huang H, Yang P, Yao B. 2015. A thermophilic β-mannanase from Neosartorya fischeri P1 with broad pH stability and significant hydrolysis ability of various mannan polymers. Food Chem. 173:283–289.
Crossref Google Scholar
 

Yang SQ, Yan QJ, Jiang ZQ, Li LT, Tian HM, Wang YZ. 2006. High-level of xylanase production by the Thermophilic Paecilomyces themophila J18 on wheat straw in solid-state fermentation. Bioresour Technol. 97:1794–1800.
Crossref Google Scholar
Mycology
Volume 8 Issue 3,
September 2017
Pages 125-134
DOI: 10.1080/21501203.2017.1337657
Cite this article:
Ahirwar S, Soni H, Prajapati BP, et al. Isolation and screening of thermophilic and thermotolerant fungi for production of hemicellulases from heated environments. Mycology, 2017, 8(3): 125-134. https://doi.org/10.1080/21501203.2017.1337657

93

Views

32

Crossref

0

Web of Science

35

Scopus

Altmetrics
Received: 10 October 2016
Accepted: 31 May 2017
Published: 16 June 2017
© 2017 The Author(s).

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Return