AI Chat Paper
Note: Please note that the following content is generated by AMiner AI. SciOpen does not take any responsibility related to this content.
{{lang === 'zh_CN' ? '文章概述' : 'Summary'}}
{{lang === 'en_US' ? '中' : 'Eng'}}
Chat more with AI
Powered by AMiner. Clicking this button will take you away from SciOpen.
Home Mycology Article
Article Link
Cite
EndNote(RIS) BibTeX
Collect
Submit Manuscript
Show Outline
Outline
Show full outline
Hide outline
Outline
Show full outline
Hide outline
Invited Article | Open Access

Oligotrophic fungi from a carbonate cave, with three new species of Cephalotrichum

Jia-Rui Jianga,b, Lei Caia, Fang Liua( )
State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China
College of Life Sciences, University of Chinese Academy of Sciences, Beijing, China
Show Author Information

Abstract

Oligotrophs are microorganisms that can grow in environments where concentrations of nutrients are low or even absent. Caves are typical oligotrophic environments distinctly characterised by constant low temperature, high humidity, scarcity of organic matter and darkness, which encompass a high diversity of fungi. In our investigation of microorganisms from carbonate caves in China, 169 strains belonging to at least 84 taxa were isolated using oligotrophic carbon free silica gel medium (SGM). Cephalotrichum appeared to be one of the dominant genera. Further morphological comparisons and molecular phylogenetic analyses using DNA sequences of four loci (LSU, ITS, TUB2 and EF-1α) revealed that the 30 strains of Cephalotrichum represent three new species, which are described and named C. guizhouense, C. laeve and C. oligotriphicum. This study also significantly improved our understanding on fungi being able to grow on carbon free medium, with the known species increased from 18 to 99.

Keywords

morphologytaxonomyphylogenyCave fungioligotrophic fungi

References

 

Barton HA, Juardo V. 2007. What’s up down there? Microbe-Am Soc Microbiol. 2:132–138.
Google Scholar
 

Bastian F, Alabouvette C, Saiz-Jimenez C. 2009. The impact of arthropods on fungal community structure in Lascaux Cave. J Appl Microbiol. 106:1456–1462.
Crossref Google Scholar
 

Bergero R, Girlanda M, Varese GC, Intili D, Luppi AM. 1999. Psychrooligotrophic fungi from arctic soils of Franz Joseph Land. Polar Biol. 21:361–368.
Crossref Google Scholar
 

Borda D, Borda C, Tămaş T. 2004. Bats, climate, and air microorganisms in a Romanian cave. Mammalia. 68:337–343.
Crossref Google Scholar
 

Burford EP, Kierans M, Gadd GM. 2003. Geomycology: fungi in mineral substrata. Mycologist. 17:98–107.
Crossref Google Scholar
 

Clements FE. 1896. Report on collections made in 1894–95. Bot Surv Nebraska. 4:1–48.
Google Scholar
 

Connell L, Staudigel H. 2013. Fungal diversity in a dark oligotrophic volcanic ecosystem (DOVE) on Mount Erebus, Antarctica. Biology. 2:798–809.
Crossref Google Scholar
 

Cubero OF, Crespo ANA, Fatehi J, Bridge PD. 1999. DNA extraction and PCR amplification method suitable for fresh, herbarium-stored, lichenized, and other fungi. Plant Syst Evol. 216:243–249.
Crossref Google Scholar
 
Gabriel CR, Northup DE. 2013. Microbial ecology: caves as an extreme habitat. Cave Microbiomes: A novel resource for drug discovery. Springer New York, p.85–108
Crossref
 

Gadd GM. 2007. Geomycology: biogeochemical transformations of rocks, minerals, metals and radionuclides by fungi, bioweathering and bioremediation. Mycol Res. 111:3–49.
Crossref Google Scholar
 

Glass NL, Donaldson GC. 1995. Development of primer sets designed for use with the PCR to amplify conserved genes from filamentous ascomycetes. Appl Environ Microb. 61:1323–1330.
Crossref Google Scholar
 

Godinho VM, Gonçalves VN, Santiago IF, Figueredo HM, Vitoreli GA, Schaefer CE, Barbosa EC, Oliveira JG, Alves TM, Zani CL, et al. 2015. Diversity and bioprospection of fungal community present in oligotrophic soil of continental Antarctica. Extremophiles. 19:585–596.
Crossref Google Scholar
 

Gundersen KR, Corbin JS, Hanson CL, Hanson ML, Hanson RB, Russell DJ, Stollar A, Yamada O. 1976. Structure and biological dynamics of the oligotrophic ocean photic zone off the Hawaiian Islands. Pac Sci. 30:45–68.
Google Scholar
 

Hiroyuki O, Tsutomu H. 1983. Oligotrophic bacteria on organic debris and plant roots in a paddy field soil. Soil Biol Biochem. 15:1–8.
Crossref Google Scholar
 

Jiang JR, Chen Q, Cai L. 2017. Polyphasic characterisation of three novel species of Paraboeremia. Mycol Prog. 16:285–295.
Crossref Google Scholar
 

Jiang YL, Zhang TY. 2008. Two new species of Doratomyces from soil. Mycotaxon. 104:131–134.
Google Scholar
 

Katoh K, Standley DM. 2013. MAFFT multiple sequence alignment software version 7: improvements in performance and usability. Mol Biol Evol. 30:772–780.
Crossref Google Scholar
 

Kiyuna T, An K, Kigawa R, Sano C, Sugiyama J. 2017. Noteworthy anamorphic fungi, Cephalotrichum verrucisporum, Cephalotrichum verrucisporum, and Sagenomella griseoviridis, isolated from biodeteriorated samples in the Takamatsuzuka and Kitora Tumuli, Nara, Japan. Mycoscience. doi:10.1016/j.myc.2017.02.003
Crossref Google Scholar
 

Kuzmina L, Galimzianova N, Abdullin S, Ryabova A. 2012. Microbiota of the Kinderlinskaya cave (South Urals, Russia). Microbiology. 81(2):251–258.
Crossref Google Scholar
 

Li W, Zhou PP, Jia LP, Yu LJ, Li XL, Zhu M. 2009. Limestone dissolution induced by fungal mycelia, acidic materials, and carbonic anhydrase from fungi. Mycopathologia. 167:37–46.
Crossref Google Scholar
 
Link HF. 1809. Observationes in Ordines plantarum naturales [Dissertatio 1ma. (Berlin Ges. NatKde 3:1–42)]. Germany: Berlin.
 

Liu TT, Hu DM, Liu F, Cai L. 2013. Polyphasic characterization of Plectosphaerella oligotrophica, a new oligotrophic species from China. Mycoscience. 54:387–393.
Crossref Google Scholar
 

Martin P, MacLeod RA. 1984. Observations on the distinction between oligotrophic and eutrophic marine bacteria. Appl Environ Microb. 47:1017–1022.
Crossref Google Scholar
 

Mason-Gamer RJ, Kellogg EA. 1996. Testing for phylogenetic conflict among molecular data sets in the tribe Triticeae (Gramineae). Syst Biol. 45:524–545.
Crossref Google Scholar
 

Mirocha CJ, DeVay JE. 1971. Growth of fungi on an inorganic medium. Can J Microbiol. 17:1373–1378.
Crossref Google Scholar
 

Northup DE, Lavoie KH. 2001. Geomicrobiology of caves: a review. Geomicrobiol J. 18:199–222.
Crossref Google Scholar
 

Nováková A. 2009. Microscopic fungi isolated from the Domica Cave system (Slovak Karst National Park, Slovakia). A review. Int J Speleol. 38(1):71–82.
Crossref Google Scholar
 

Parkinson SM, Killham K, Wainwright M. 1990. Assimilation of 14CO2 by Fusarium oxysporum grown under oligotrophic conditions. Mycol Res. 94:959–964.
Crossref Google Scholar
 

Parkinson SM, Wainwright M, Killham K. 1989. Observations on oligotrophic growth of fungi on silica gel. Mycol Res. 93:529–534.
Crossref Google Scholar
 

Payton M, McCullough W, Roberts CF. 1976. Agar as a carbon source and its effect on the utilization of other carbon sources by acetate non-utilizing (acu) mutants of Aspergillus nidulans. J Gen Microbiol. 94:228–233.
Crossref Google Scholar
 

Poindexter JS. 1981. Oligotrophy. Adv Microb Ecol. 5:63–89.
Crossref Google Scholar
 

Posada D. 2008. jModelTest: phylogenetic model averaging. Mol Biol Evol. 25:1253–1256.
Crossref Google Scholar
 
Rayner RW. 1970. A mycological colour chart. UK: Commonwealth Mycological Institute and British Mycological Society.
 

Rehner SA, Buckley E. 2005. A Beauveria phylogeny inferred from nuclear ITS and EF1-α sequences: evidence for cryptic diversification and links to Cordyceps teleomorphs. Mycologia. 97:84–98.
Crossref Google Scholar
 

Rehner SA, Samuels GJ. 1994. Taxonomy and phylogeny of Gliocladium analysed from nuclear large subunit ribosomal DNA sequences. Mycol Res. 98:625–663.
Crossref Google Scholar
 

Ronquist F, Teslenko M, van der Mark P, Ayres DL, Darling A, Höhna S, Larget B, Liu L, Suchard MA, Huelsenbeck JP. 2012. MrBayes 3.2: efficient Bayesian phylogenetic inference and model choice across a large model space. Syst Biol. 61:539–542.
Crossref Google Scholar
 

Ruibal C, Platas G, Bills GF. 2005. Isolation and characterization of melanized fungi from limestone formations in Mallorca. Mycol Prog. 4:23–38.
Crossref Google Scholar
 

Sandoval-Denis M, Gené J, Sutton DA, Cano-Lira JF, de Hoog GS, Decock CA, Wiederhold NP, Guarro J. 2016a. Redefining Microascus, Scopulariopsis and allied genera. Persoonia. 36:1–36.
Crossref Google Scholar
 

Sandoval-Denis M, Guarro J, Cano-Lira JF, Sutton DA, Wiederhold NP, de Hoog GS, Abbott SP, Decock C, Sigler L, Gené J. 2016b. Phylogeny and taxonomic revision of Microascaceae with emphasis on synnematous fungi. Stud Mycol. 83:193–233.
Crossref Google Scholar
 

Stamatakis A, Alachiotis N. 2010. Time and memory efficient likelihood-based tree searches on phylogenomic alignments with missing data. Bioinformatics. 26:i132–i139.
Crossref Google Scholar
 

Sterflinger K. 2000. Fungi as geologic agents. Geomicrobiol J. 17:97–124.
Crossref Google Scholar
 

Sturm J. 1829. Deutschlands Flora, Abt. Ⅲ. Die Pilze Deutschlands. 2:1–136.
Google Scholar
 

Tamura K, Stecher G, Peterson D, Filipski A, Kumar S. 2013. MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol. 30:2725–2729.
Crossref Google Scholar
 

Udagawa S, Horie Y, Abdullah SK. 1985. Trichurus dendrocephalus sp. nov., from Iraqui soil. Mycotaxon. 23:253–259.
Google Scholar
 

Vilgalys R, Hester M. 1990. Rapid genetic identification and mapping of enzymatically amplified ribosomal DNA from several Cryptococcus species. J Bacteriol. 172:4238–4246.
Crossref Google Scholar
 

Wainwright M, Al-Talhi A. 1999. Selective isolation and oligotrophic growth of Candida on nutrient-free silica gel medium. J Med Microbiol. 48:1130.
Crossref Google Scholar
 

Wainwright M, Al-Wajeeh K, Grayston SJ. 1997. Effect of silicic acid and other silicon compounds on fungal growth in oligotrophic and nutrient-rich media. Mycol Res. 101:933–938.
Crossref Google Scholar
 

Wainwright M, Grayston SJ. 1988. Fungal growth and stimulation by thiosulphate under oligocarbotrophic conditions. Tr Brit Mycol Soc. 91:149–156.
Crossref Google Scholar
 
Wainwright M. 1993. Oligotrophic growth of fungi: stress or natural state? In: Jennings DH, editor. Stress tolerance of fungi. New York: Marcel Dekker; p. 127–144.
 
White TJ, Bruns T, Lee S, Taylor JW. 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis MA, Gelfand DH, Sninsky JJ, White TJ, editors. PCR protocols: a guide to methods and applications. San Diego, CA: Academic Press; p. 315–322.
Crossref
 

Zhang Y, Liu F, Wu W, Cai L. 2015. A phylogenetic assessment and taxonomic revision of the thermotolerant hyphomycete genera Acrophialophora and Taifanglania. Mycologia. 107:768–779.
Crossref Google Scholar
Mycology
Volume 8 Issue 3,
September 2017
Pages 164-177
DOI: 10.1080/21501203.2017.1366370
Cite this article:
Jiang J-R, Cai L, Liu F. Oligotrophic fungi from a carbonate cave, with three new species of Cephalotrichum. Mycology, 2017, 8(3): 164-177. https://doi.org/10.1080/21501203.2017.1366370

141

Views

26

Crossref

0

Web of Science

32

Scopus

Altmetrics
Received: 28 April 2017
Accepted: 01 August 2017
Published: 24 August 2017
© 2017 The Author(s).

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Return