AI Chat Paper
Note: Please note that the following content is generated by AMiner AI. SciOpen does not take any responsibility related to this content.
{{lang === 'zh_CN' ? '文章概述' : 'Summary'}}
{{lang === 'en_US' ? '中' : 'Eng'}}
Chat more with AI
Powered by AMiner. Clicking this button will take you away from SciOpen.
Home Mycology Article
Article Link
Cite
EndNote(RIS) BibTeX
Collect
Submit Manuscript
Show Outline
Outline
Show full outline
Hide outline
Outline
Show full outline
Hide outline
Article | Open Access

Isolation and characterization of the bioactive metabolites from the soil derived fungus Trichoderma viride

Nagwa E. Awada,, Hanaa A. Kassemb, Manal A. Hamedc( ), Amal M. El-Fekya, Mohamed A. A. Elnaggard, Khaled Mahmouda, Mohamed A. Alie
Pharmacognosy Department, National Research Centre, Giza, Egypt
Pharmacognosy Department, Faculty of Pharmacy, Cairo University, Giza, Egypt
Therapeutic Chemistry Department, National Research Centre, Giza, Egypt
Plant Pathology Department, National Research Centre, Giza, Egypt
Center of Scientific Excellence for Influenza Viruses, National Research Centre, Dokki, Giza, Egypt

This article was originally published with error. This version has been corrected. Please see Corrigendum (https://doi.org/10.1080/21501203.2018.1505371).

Show Author Information

Abstract

The aim of the present study was to evaluate different biological activities of Trichoderma viride fungus (Family Hypocreaceae). Trichoderma viride isolated for the first time from the cucumber soil (rhizosphere). It was tested as antimicrobial, antioxidant and anticancer agent. Trichoderma viride from the cucumber soil (rhizosphere) caused inhibition of the mycelial growth of Fusarium solani, Rhizoctonia solani and Sclerotium rolfsii. Also, the alcoholic extract of the fungal mycelia proved a potent antibacterial activity against Bacillus subtilis, Escherichia coli and Pseudomonas fluorescens. In addition, it exhibited a significant antifungal activity against Candida albicans, Fusarium solani, Fusarium oxysporium, Rhizoctonia solani and Pythium ultimum at 100 µg/disc. Study of the antimicrobial and antioxidant activities of the volatile constituents had been done. The in vitro antioxidant, anticancer and antiviral activities of the isolated proteins, and carbohydrates were determined. Furthermore, the volatile constituents were isolated from fresh mycelia of Trichoderma viride and subjected to GC/MS analysis. Total protein (10%), carbohydrate (19.57%), steroidal (13.95%) and triterpenoidal content (38.34%) were determined in the alcoholic extract of Trichoderma viride mycelia. In conclusion, this fungus showed antioxidant, anticancer, antiviral and antibacterial effects. Further studies must be done to identify the molecules responsible for its effect and to consider its application in the pharmacological and medicinal purposes.

Keywords

antioxidantantimicrobialanticancerantiviralTrichoderma viride

References

 

Abd El-Rahman AA, ElShafei SMA, Ivanova EV, Fattakhova AN, Pankova AV, El-Shafei MA, El- El-Morsi MA, Alimova FK. 2014. Cytotoxicity of Trichoderma spp. cultural filtrate against human cervical and breast cancer cell lines. Asian Pac J Cancer Prev. 15:7229–7234.
Crossref Google Scholar
 

Adams P. 1989. Identification of essential oils by ion trap mass spectroscopy. New York: Academic Press, INC.
Crossref
 

Ait-Lahsen H, Soler A, Rey M, De La Cruz J, Monte E, Liobell A. 2001. An antifungal exo-ᾳ-1,3-glucanase (AGN13.1) from the biocontrol fungus Trichoderma harzianum. Appl Environ Microbiol. 67:5833–5839.
Crossref Google Scholar
 

Ajith PS, Lakshmidevi N. 2010. Effect of volatile and non-volatile compounds from Trichoderma spp. against Colletotrichum capsici incitant of anthracnose on bell peppers. Nat Sci. 8:265–269.
Google Scholar
 
Barnett HL, Hunter BB. 1972. Illustrated genera of imperfect fungi. Minneapolis: Burgess Publ. com.; p. 241.
 
Baron S. 1996. Introduction to Mycology. 4th ed. Galveston: University of Texas Medical Branch.
 

Brito-Vega H, Espinosa-Victoria D, Salaya-Domínguez JM, Gómez-Méndez1 E. 2013. The soil biota: importance in agroforestry and agricultural systems. Tropical and Subtropical Agroecosystems. 16:445–453.
Google Scholar
 

Chen Y, Wang M, Rosen RT, Ho CT. 1999. 2,2-Diphenyl-2-picrylhydrazyl radical-scavenging active components from Polygonum multiflorum Thunb. J Agric Food Chem. 47:2226–2228.
Crossref Google Scholar
 
Chet I, Inbar J, Hadar I. 1997. Fungal antagonists and mycoparasites. In: Wicklow DT, Soderstorm B, eds.. Berlin: Springer, The Mycota Ⅳ: environmental and microbial relationships. p. 165–184.
 

De La Cruz J, Llobell A. 1999. Purification and properties of a basic endo-b-1,6-glucanase (BGN16.1) from the antagonistic fungus Trichoderma harzianum. Eur J Biochem. 265:145–151.
Crossref Google Scholar
 

De La Cruz J, Pintor-Toro JA, Benitez T, Llobell A. 1995. Purification and characterization of an endo-b-1,6-glucanase from Trichoderma harzianum that is related to its mycoparasitism. J Bacteriol. 177:1864–1871.
Crossref Google Scholar
 
Demain AL, Fang A. 2000. The natural function of secondary metabolites. Advanced in Biochemical engineering/Biochemistry. Heidelberg (Germany): Springer; p. 1–39.
Crossref
 

Dennis C, Webster J. 1971. Antagonistic properties of species groups of Trichoderma: Ⅲ. Hyphal interactions. Trans Br Mycol Soc. 57:363–369.
Crossref Google Scholar
 

Dubois M, Gilles KA, Hamilton JK, Rebers PA, Smith F. 1956. Colorimetric method for determination of sugars and related substances. AnalChem. 28:350–356.
Crossref Google Scholar
 

El-Gengaihi S, Karawya M, Selim M, Motawe H, Ibrahim N. 1996. Chemical and biological investigation of polypeptides of Monordica and Luffa Dpp. Fam. Cucurbitaceae. J Bull NRC. 21:269–276.
Google Scholar
 

Feofilova EP. 2001. The kingdom fungi: heterogeneity of physiological and biochemical properties and relationships with plants, animals, and prokaryotes. Appl Biochemistry Microbiol. 37:124–137.
Crossref Google Scholar
 

Gajera HP, Katakpara ZA, Patel SV, Golakiya BA. 2016. Antioxidant defense response induced by Trichoderma viride against Aspergillus niger Van Tieghem causing collar rot in groundnut (Arachis hypogaea L.). Microb Pathog. 91:26e–34.
Crossref Google Scholar
 

Geremia RA, Goldman GH, Jacobs D, Ardiles W, Vila SB, Van Montagu M, Herreraestrella A. 1993. Molecular characterization of the proteinase-encoding gene, prb1, related to mycoparasitism by Trichoderma harzianum. Mol Microbiol. 8:603–613.
Crossref Google Scholar
 

Gnanamanickam SS, Mansfield JW. 1981. Selective toxicity of wyerone and other phytoalexins to gram-positive bacteria. Phytochemistry. 20:997–1000.
Crossref Google Scholar
 

Harman GE. 2006. Overview of mechanisms and uses of Trichoderma spp. Phytopathology. 96:190–194.
Crossref Google Scholar
 

Hawksworth DL. 1991. The fungal dimension of biodiversity: magnitude, significance, and conservation. Mycol Res. 95:641–655.
Crossref Google Scholar
 

Hayden FG, Cote KM, Douglas RG. 1980. Plaque inhibition assay for drug susceptibilitytesting of influenza viruses. Antimicrob Agents Chemother. 17:865–870.
Crossref Google Scholar
 

Hoell IA, Klemsdal SS, Vaaje-Kolstad G, Horn SJ, Eijsink VGH. 2005. Overexpression and characterization of a novel chitinase from Trichoderma atroviride strain P1. Biochim Biophys Acta. 1748:180–186.
Crossref Google Scholar
 

Keszler A, Forgacs E, Kotal L, Vizcaino JA, Monte E, Garcia-Acha I. 2000. Separation and identification of volatile components in the fermentation broth of Trichoderma atroviride by solidphase extraction and gas chromatography-mass spectroscopy. J Chromatograph Sci. 38:421–424.
Crossref Google Scholar
 
Kirk PM, Cannon PF, Minter DW, Stalpers JA. 2011. Dictionary of the fungi. 10th ed. UK: Wallingford; p. 131.
 

Kredics L, Zsuzsanna A, Szekeres A, Hatvani L, Manczinger L, Cs V, Erzsebet N. 2005. Extracellular proteases of Trichoderma species-a review. Acta Microbiol Immunol Hung. 52:169–184.
Crossref Google Scholar
 

Kubicek CP, Mach RL, Peterbauer CK, Lorito M. 2001. Trichoderma: from genes to biocontrol. J Plant Pathol. 83:11–23.
Google Scholar
 

Laidlow M, Percival V. 1950. The Chemistry of Gum and Mucilage. New York: Rembold Publishing Co.
 

Lieckfeldt E, Samuels GJ, Nirenberg HI, Petrini O. 1999. A morphological and molecular perspective of Trichoderma viride: is it one or two species. Appl Environ Microbiol. 65:2418–2428.
Crossref Google Scholar
 

Macleod AJ, Cave SJJ. 1975. Volatile flavour components of egg. Sci Food Agric. 26:351–358.
Crossref Google Scholar
 

Mannina L, Segrc AL. 1997. A new fungal growth inhibitor from Trichoderma viride. Tetrahedron. 53:3135–3144.
Crossref Google Scholar
 

Meyer V. 2008. Genetic engineering of filamentous fungi-Progress, obstacles and future trends. Biotechnol Adv. 26:177–185.
Crossref Google Scholar
 

Mosmann T. 1983. Rapid colorimetric assays for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods. 65:55–63.
Crossref Google Scholar
 
Pearson D. 1970. The chemical analysis of foods. 6th ed. London: Churchill LTD.; p. 9.
 

Pozo MJ, Baek JM, Garcia JM, Kenerley CM. 2004. Functional analysis of tvsp1, a serine protease-encoding gene in the biocontrol agent Trichoderma virens. Fungal Genet Biol. 41:336–348.
Crossref Google Scholar
 
Ramirez C. 1982. Manual and Atlas of the Penicilla. Amesterdam (New York, Oxford): Elserier Biomedical Press; p. 123.
 

Respinis SD, Vogel G, Benagli C. 2010. MALDI-TOF MS of Trichoderma: model system for the identification of microfungi. Mycol Prog. 9:79–100.
Crossref Google Scholar
 
Sivasithamparam K, Ghisalberti EL. 1998. Secondary metabolism in Trichoderma and Gliocladium. Trichoderma and Gliocladium. Vol.1, Basic Biology, Taxonomy and Genetics. London: Taylor and Francis Ltd; p. 139–191.
 

Soytong K, Quimio TH. 1989. Antagonism of Chaetomium globosum to the rice blast pathogen. Pyricularia Oryzae Kasetsart J Nat Sci. 23:198–203.
Google Scholar
 

St-Germain G, Summerbell R. 1996. Identifying filamentous fungi - a clinical laboratory handbook. 1st ed. Belmont (California): Star Publishing Company.
 

Sutton DA, Fothergill AW, Rinaldi MG. 1998. Guide to clinically significant fungi. 1st ed. Baltimore: Williams & Wilkins.
 

SwiftMary L. 1984. Analysis of molluscan sterols: colorimetric methods. Lipids. 19:625–630.
Crossref Google Scholar
 

Thabrew MI, Hughes RD, McFarlane IG. 1997. Screening of hepatoprotective plant components using a HepG2 cell cytotoxicity assay. J Pharm Pharmacol. 49:1132–1135.
Crossref Google Scholar
 

Weinding R. 1934. Studies on a lethal principle effective in the parasitic action of Trichoderma lignorum on Rhizoctonia solani and other soil fungi. Phytopath. 24:1153–1179.
Google Scholar
 

Widner K, Eggum OB. 1966. Protein analysis. A description of the method used at the of animal physiology in Copenhagen. Acta Agricultura Scandinavi. 16:15.
Crossref Google Scholar
 

Zeilinger S, Galhaup C, Payer K, Woo SL, Mach RL, Fekete C, Lorito M, Kubicek CP. 1999. Chitinase gene expression during mycoparasitic interaction of Trichoderma harzianum with its host. Fungal Genet Biol. 26:131–140.
Crossref Google Scholar
Mycology
Volume 9 Issue 1,
March 2018
Pages 70-80
DOI: 10.1080/21501203.2017.1423126
Cite this article:
Awad NE, Kassem HA, Hamed MA, et al. Isolation and characterization of the bioactive metabolites from the soil derived fungus Trichoderma viride. Mycology, 2018, 9(1): 70-80. https://doi.org/10.1080/21501203.2017.1423126

248

Views

51

Crossref

0

Web of Science

56

Scopus

Altmetrics
Received: 14 October 2017
Accepted: 28 December 2017
Published: 10 January 2018
© 2018 The Author(s).

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Return