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Article | Open Access

Studies on the antifungal and serotonin receptor agonist activities of the secondary metabolites from piezotolerant deep-sea fungus Ascotricha sp.

A. Ganesh Kumara( ), K. Balamuruganb, R. Vijaya Raghavana, G. Dharania, R. Kirubagarana
Marine Biotechnology Division, Ocean Science and Technology for Islands Group, ESSO – National Institute of Ocean Technology, Ministry of Earth Sciences (MoES), Government of India, Chennai, Tamilnadu, India
Department of Biotechnology, Alagappa University, Karaikudi, Tamilnadu, India
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Abstract

The potent antifungal agent sesquiterpenes and serotonin 5-HT2C agonist ascotricin were produced by a newly isolated deep-sea fungus Ascotricha sp. This fungus was isolated from deep-sea sediment collected at a depth of 1235 m and characterized. Piezotolerance was successfully tested under high pressure-low temperature (100 bar pressure and 20℃) microbial cultivation system. Production of secondary metabolites was enhanced at optimized culture conditions. The in-vivo antifungal activity of sesquiterpenes was studied using the Caenorhabditis elegans – Candida albicans model system. The sesquiterpenes affected the virulence of C. albicans and prolonged the life of the host C. elegans. These findings suggest that sesquiterpenes are attractive antifungal drug candidates. The 5-HT2C receptor agonist is a potential target for the development of drugs for a range of central nervous system disorders. The interaction of 5-HT2C agonist ascotricin with the receptor was studied through bioinformatic analysis. The in silico molecular docking and molecular dynamic simulation studies demonstrated that they fit into the serotonin 5-HT2C active site and the crucial amino acid residues involved in the interactions were identified. To our knowledge, this is first report of in vivo antifungal analysis of sesquiterpenes and in silico studies of serotonin 5-HT2C receptor-ascotricin complex.

Keywords

Candida albicansCaenorhabditis eleganssesquiterpenesAscotricha sp5-HT2C receptor agonist

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Mycology
Volume 10 Issue 2,
June 2019
Pages 92-108
DOI: 10.1080/21501203.2018.1541934
Cite this article:
Kumar AG, Balamurugan K, Raghavan RV, et al. Studies on the antifungal and serotonin receptor agonist activities of the secondary metabolites from piezotolerant deep-sea fungus Ascotricha sp.. Mycology, 2019, 10(2): 92-108. https://doi.org/10.1080/21501203.2018.1541934

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Received: 06 September 2018
Accepted: 23 October 2018
Published: 21 November 2018
© 2018 The Author(s).

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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