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Research Article | Open Access

Influence of plant growth-promoting endophytes Colletotrichum siamense and Diaporthe masirevici on tomato plants (Lycopersicon esculentum Mill.)

Shalene da Silva Santosa, Angela Aparecida da Silvaa, Julio Cesar Polonioa ( ), Andressa Domingos Pollia, Ravely Casarotti Orlandellia, João Arthur dos Santos Oliveiraa, José Usan Torres Brandão-Filhob, João Lúcio Azevedoc, João Alencar Pamphilea,†,
Department of Biotechnology, Genetics and Cell Biology, Universidade Estadual de Maringá, Maringá, Brazil
Department of Agronomy, Universidade Estadual de Maringá, Maringá, Brazil
Department of Genetics, College of Agriculture Luiz de Queiroz, Universidade de São Paulo, Piracicaba, Brazil

†in memoriam

This article has been corrected with minor changes. These changes do not impact the academic content of the article.

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Abstract

The protective and growth-promoting activities of Colletrotrichum and Diaporthe endophytes on tomato plants (Lycopersicon esculentum Mill.) are underexplored. We screened 40 endophytic fungi associated with Mexican shrimp plant (Justicia brandegeana) using an in vitro dual culture assay for Fusarium oxysporum, one of the most important phytopathogens of tomato plants. The three best antagonists, Colletotrichum siamense (JB224.g1), C. siamense (JB252.g1), and Diaporthe masirevicii (JB270), were identified based on multilocus sequence analysis. They were assessed in vitro for their inhibition of F. oxysporum and phosphate solubilisation capacity, and for the production of indole acetic acid. Greenhouse experiments verified the growth-promoting effects of these endophytes and the suppression of F. oxysporum symptoms in tomato plants. Under greenhouse conditions, the JB252.g1 and JB270 isolates showed positive results for seedling emergence speed. The radicular system depth of plants inoculated with JB270 was greater than that in uninoculated plants (27.21 vs 21.95 cm). The soil plant analysis development chlorophyll metre (SPAD) index showed statistically significant results, especially for the endophyte JB224.g1 (36.99) compared to the control plants (30.90) and plants infected solely with F. oxysporum (33.64).

Keywords

fusarium oxysporumEndophytesphosphate solubilisationIAA productionSPAD index

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Mycology
Volume 13 Issue 4,
December 2022
Pages 257-270
DOI: 10.1080/21501203.2022.2050825
Cite this article:
da Silva Santos S, da Silva AA, Polonio JC, et al. Influence of plant growth-promoting endophytes Colletotrichum siamense and Diaporthe masirevici on tomato plants (Lycopersicon esculentum Mill.). Mycology, 2022, 13(4): 257-270. https://doi.org/10.1080/21501203.2022.2050825

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Received: 03 March 2022
Accepted: 03 March 2022
Published: 17 March 2022
© 2022 The Author(s).

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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