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Topical Review | Open Access

Biofabrication strategies with single-cell resolution: a review

Dezhi Zhou1,2,3Bohan Dou1,2,3Florian Kroh1,2,3Chuqian Wang1,2,3Liliang Ouyang1,2,3 ( )
Department of Mechanical Engineering, Tsinghua University, Beijing 100084, People’s Republic of China
Biomanufacturing and Rapid Forming Technology Key Laboratory of Beijing, Tsinghua University, Beijing 100084, People’s Republic of China
‘Biomanufacturing and Engineering Living Systems’ Innovation International Talents Base (111 Base), Tsinghua University, Beijing 100084, People’s Republic of China
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Abstract

The introduction of living cells to manufacturing process has enabled the engineering of complex biological tissues in vitro. The recent advances in biofabrication with extremely high resolution (e.g. at single cell level) have greatly enhanced this capacity and opened new avenues for tissue engineering. In this review, we comprehensively overview the current biofabrication strategies with single-cell resolution and categorize them based on the dimension of the single-cell building blocks, i.e. zero-dimensional single-cell droplets, one-dimensional single-cell filaments and two-dimensional single-cell sheets. We provide an informative introduction to the most recent advances in these approaches (e.g. cell trapping, bioprinting, electrospinning, microfluidics and cell sheets) and further illustrated how they can be used in in vitro tissue modelling and regenerative medicine. We highlight the significance of single-cell-level biofabrication and discuss the challenges and opportunities in the field.

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International Journal of Extreme Manufacturing
Article number: 042005
Cite this article:
Zhou D, Dou B, Kroh F, et al. Biofabrication strategies with single-cell resolution: a review. International Journal of Extreme Manufacturing, 2023, 5(4): 042005. https://doi.org/10.1088/2631-7990/ace863

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Received: 14 February 2023
Revised: 28 March 2023
Accepted: 18 July 2023
Published: 09 August 2023
© 2023 The Author(s).

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