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Establishment of Insulin Resistance Model of HepG2 Cells and Application in Selection of Active Natural Polysaccharides

Wei JIANG1, Qian SHI2, Xiaoying LIU1, Yulan FU1, Jinyao LI1, Weilan WANG1()
Xinjiang Key Laboratory of Biological Resources and Genetic Engineering, School of Life Science and Technology, Xinjiang University, Urumqi Xinjiang 830017, China
Department of Clinical Laboratory, Hospital of Xinjiang Production and Construction Corps, Urumqi Xinjiang 830063, China
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Abstract

To explore the establishment of insulin resistance model of HepG2(IR-HepG2) induced by high glucose, dexamethasone and insulin, to detect the effect of Pleurotus eryngii polysaccharides, Ziziphus jujuba polysaccharides and Lycium barbarum polysaccharides on improving insulin resistance of liver cells in vitro, and to analyze the effect of three polysaccharides on hypoglycemic. Different concentrations of dexamethasone, insulin and their combination were selected to induce the insulin resistance of HepG2 cells in high glucose culture. MTT method was used to detect the effect of different inducers on the activity of HepG2 cells. Glucose oxidase method was used to detect the glucose content in the supernatant of cell culture. At the same time, the stability of IR-HepG2 cell model was detected after removing inducers. IR-HepG2 cells were treated with three kinds of natural polysaccharides at different concentrations. The effects of polysaccharides on cell viability and insulin resistance were detected by MTT and glucose oxidase methods respectively. Dexamethasone(3.75 μmol/L) combined with insulin(0.001 μmol/L) can successfully induce IR-HepG2 cell model in high glucose culture condition, and has no significant effect on cell activity, and the model can keep stable within 72 hours. Lycium barbarum polysaccharides had better hypoglycemic effects in vitro.

Keywords

insulin resistanceHepG2 cellsPleurotus eryngii polysaccharidesZiziphus jujuba polysaccharidesLycium barbarum polysaccharides
CLC number: TS201.4 Document code: A Article ID: 2096-7675(2023)04-0461-06

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Journal of Xinjiang University(Natural Science Edition in Chinese and English)
Volume 40 Issue 4,
July 2023
Pages 461-466
DOI: 10.13568/j.cnki.651094.651316.2022.12.22.0001
Cite this article:
JIANG W, SHI Q, LIU X, et al. Establishment of Insulin Resistance Model of HepG2 Cells and Application in Selection of Active Natural Polysaccharides. Journal of Xinjiang University(Natural Science Edition in Chinese and English), 2023, 40(4): 461-466. https://doi.org/10.13568/j.cnki.651094.651316.2022.12.22.0001
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