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Advances on the Biological Function of Major Latex Proteins(MLPs) in Plants

School of Life Science and Technology, Xinjiang University, Urumqi Xinjiang 830017, China
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Abstract

Major latex proteins(MLPs) are a type of plant-specific proteins that primarily exist in dicotyledonous and some monocotyledonous plants. They play important roles in plant growth and development, as well as in defense against biotic and abiotic stresses. This article discusses the spatial structure, biological functions, and mechanisms of action of MLPs, providing references for further understanding of MLP family members and their applications in crop improvement.

CLC number: Q71 Document code: A Article ID: 2096-7675(2025)01-0073-09

References

[1]
NESSLER C L, KURZ W G, PELCHER L E. Isolation and analysis of the major latex protein genes of opium poppy[J]. Plant Molecular Biology, 1990, 15(6): 951-953.
[2]
WANG Y P, YANG L, CHEN X, et al. Major latex protein-like protein 43 (MLP43) functions as a positive regulator during abscisic acid responses and confers drought tolerance in Arabidopsis thaliana[J]. Journal of Experimental Botany, 2016, 67(1): 421-434.
[3]
SONG L Y, WANG J, JIA H Y, et al. Identification and functional characterization of NbMLP28, a novel MLP-like protein 28 enhancing Potato virus Y resistance in Nicotiana benthamiana[J]. BMC Microbiology, 2020, 20(1): 55.
[4]
HE S S, YUAN G P, BIAN S X, et al. Major latex protein MdMLP423 negatively regulates defense against fungal infections in apple[J]. International Journal of Molecular Sciences, 2020, 21(5): 1879.
[5]
ZENG J X, RUAN Y X, LIU B Y, et al. Genome-wide identification and abiotic stress-responsive expression of MLP family genes in Brassica rapa[J]. Gene Reports, 2020, 21: 100919.
[6]
ZHANG N B, LI R M, SHEN W, et al. Genome-wide evolutionary characterization and expression analyses of major latex protein (MLP) family genes in Vitis vinifera[J]. Molecular Genetics and Genomics, 2018, 293(5): 1061-1075.
[7]
IWABUCHI A, KATTE N, SUWA M, et al. Factors regulating the differential uptake of persistent organic pollutants in cucurbits and non-cucurbits[J]. Journal of Plant Physiology, 2020, 245: 153094.
[8]
RADAUER C, LACKNER P, BREITENEDER H. The Bet v1 fold: An ancient, versatile scaffold for binding of large, hydrophobic ligands[J]. BMC Evolutionary Biology, 2008, 8: 286.
[9]
GAJHEDE M, OSMARK P, POULSEN F M, et al. X-ray and NMR structure of Bet v1, the origin of birch pollen allergy[J]. Nature Structural Biology, 1996, 3(12): 1040-1045.
[10]
XIE C Z, LIU H Y, LI L, et al. Advances on class 10 pathogenesis-related proteins[J]. Molecular Plant Breeding, 2008, 6(5): 949-953. (in Chinese)
[11]
PASTERNAK O, BUJACZ G D, FUJIMOTO Y, et al. Crystal structure of Vigna radiata cytokinin-specific binding protein in complex with zeatin[J]. The Plant Cell, 2006, 18(10): 2622-2634.
[12]
LYTLE B L, SONG J K, DE LA CRUZ N B, et al. Structures of two Arabidopsis thaliana major latex proteins represent novel helix-grip folds[J]. Proteins: Structure, Function, and Bioinformatics, 2009, 76(1): 237-243.
[13]
CHOI S H, HONG M K, KIM H J, et al. Structure of ginseng major latex-like protein 151 and its proposed lysophosphatidic acid-binding mechanism[J]. Acta Crystallographica. Section D, Structural Biology, 2015, 71(Pt 5): 1039-1050.
[14]
SUN H, KIM M K, PULLA R K, et al. Isolation and expression analysis of a novel major latex-like protein (MLP151) gene from Panax ginseng[J]. Molecular Biology Reports, 2010, 37(5): 2215-2222.
[15]
MATTILA K, RENKONEN R. Modelling of Bet v1 binding to lipids[J]. Scandinavian Journal of Immunology, 2009, 70(2): 116-124.
[16]
GOTO J, IWABUCHI A, YOSHIHARA R, et al. Uptake mechanisms of polychlorinated biphenyls in Cucurbita pepo via xylem sap containing major latex-like proteins[J]. Environmental and Experimental Botany, 2019, 162: 399-405.
[17]
SARASTE M, SIBBALD P R, WITTINGHOFER A. The P-loop: A common motif in ATP- and GTP-binding proteins[J]. Trends in Biochemical Sciences, 1990, 15(11): 430-434.
[18]
BANTIGNIES B, SÉGUIN J, MUZAC I, et al. Direct evidence for ribonucleolytic activity of a PR-10-like protein from white lupin roots[J]. Plant Molecular Biology, 2000, 42(6): 871-881.
[19]
LI R. Investigation on major latex protein423 (MdMLP423) from apple in response to abiotic stress[D]. Taian: Shandong Agricultural University, 2018. (in Chinese)
[20]
FLORES T, ALAPE-GIRÓN A, FLORES-DÍAZ M, et al. Ocatin. A novel tuber storage protein from the Andean tuber crop oca with antibacterial and antifungal activities[J]. Plant Physiology, 2002, 128(4): 1291-1302.
[21]
GUO D, WONG W S, XU W Z, et al. Cis-cinnamic acid-enhanced 1 gene plays a role in regulation of Arabidopsis bolting[J]. Plant Molecular Biology, 2011, 75(4/5): 481-495.
[22]
LITHOLDO C G, PARKER B L, EAMENS A L, et al. Proteomic identification of putative microRNA394 target genes in Arabidopsis thaliana identifies major latex protein family members critical for normal development[J]. Molecular & Cellular Proteomics, 2016, 15(6): 2033-2047.
[23]
SHARMA N, ARRIGONI G, EBINEZER L B, et al. A proteomic and biochemical investigation on the effects of sulfadiazine in Arabidopsis thaliana[J]. Ecotoxicology and Environmental Safety, 2019, 178: 146-158.
[24]
POZUETA-ROMERO J, KLEIN M, HOULNÉ G, et al. Characterization of a family of genes encoding a fruit-specific wound-stimulated protein of bell pepper (Capsicum annuum): Identification of a new family of transposable elements[J]. Plant Molecular Biology, 1995, 28(6): 1011-1025.
[25]
HOLMQUIST L, DÖLFORS F, FOGELQVIST J, et al. Major latex protein-like encoding genes contribute to Rhizoctonia solani defense responses in sugar beet[J]. Molecular Genetics and Genomics, 2021, 296(1): 155-164.
[26]
LI P F, CHEN L, ZHOU Y H, et al. Brassinosteroids-induced systemic stress tolerance was associated with increased transcripts of several defence-related genes in the phloem in Cucumis sativus[J]. PLoS One, 2013, 8(6): e66582.
[27]
CHEN J Y, DAI X F. Cloning and characterization of the Gossypium hirsutum major latex protein gene and functional analysis in Arabidopsis thaliana[J]. Planta, 2010, 231(4): 861-873.
[28]
LI S L. Proteomics analysis of cotton and whitefly interaction and functional analysis of GhMLP423 gene[D]. Wuhan: Huazhong Agricultural University, 2016. (in Chinese)
[29]
YANG C L, LIANG S, WANG H Y, et al. Cotton major latex protein 28 functions as a positive regulator of the ethylene responsive factor 6 in defense against Verticillium dahliae[J]. Molecular Plant, 2015, 8(3): 399-411.
[30]
CONG R. Functional analysis of GmMMK2, GmPP1, and GmMLP genes in response to drought and salt stress[D]. Harbin: Northeast Agricultural University, 2017. (in Chinese)
[31]
LYU Q, ZHANG L, ZAN T, et al. Wheat RING E3 ubiquitin ligase TaDIS1 degrade TaSTP via the 26S proteasome pathway[J]. Plant Science, 2020, 296: 110494.
[32]
SONG L Y, JIAO Y B, SONG H P, et al. NbMLP43 ubiquitination and proteasomal degradation via the light responsive factor NbBBX24 to promote viral infection[J]. Cells, 2023, 12(4): 590.
[33]
ZHANG Y. Proteomic analysis of tobacco lines with different resistance to brown spot (Alternaria alternate) and functional study of NbMLP423[D]. Beijing: Chinese Academy of Agricultural Sciences, 2019. (in Chinese)
[34]
LIU H, MA X C, LIU S H, et al. The Nicotiana tabacum L. major latex protein-like protein 423 (NtMLP423) positively regulates drought tolerance by ABA-dependent pathway[J]. BMC Plant Biology, 2020, 20(1): 475.
[35]
GAI Y P, YUAN S S, LIU Z Y, et al. Integrated phloem sap mRNA and protein expression analysis reveals phytoplasma-infection responses in mulberry[J]. Molecular & Cellular Proteomics, 2018, 17(9): 1702-1719.
[36]
AGGELIS A, JOHN I, KARVOUNI Z, et al. Characterization of two cDNA clones for mRNAs expressed during ripening of melon (Cucumis melo L.) fruits[J]. Plant Molecular Biology, 1997, 33(2): 313-322.
[37]
SUYAMA T, YAMADA K, MORI H, et al. Cloning cDNAs for genes preferentially expressed during fruit growth in cucumber[J]. Journal of the American Society for Horticultural Science, 1999, 124(2): 136-139.
[38]
NAM Y W, TICHIT L, LEPERLIER M, et al. Isolation and characterization of mRNAs differentially expressed during ripening of wild strawberry (Fragaria vesca L.) fruits[J]. Plant Molecular Biology, 1999, 39(3): 629-636.
[39]
DAVIES C, ROBINSON S P. Differential screening indicates a dramatic change in mRNA profiles during grape berry ripening. Cloning and characterization of cDNAs encoding putative cell wall and stress response proteins[J]. Plant Physiology, 2000, 122(3): 803-812.
[40]
NEALE A D, WAHLEITHNER J A, LUND M, et al. Chitinase, β-1,3-glucanase, osmotin, and extensin are expressed in tobacco explants during flower formation[J]. The Plant Cell, 1990, 2(7): 673-684.
[41]
CHONG S N, RAVINDRAN P, KUMAR P P. Regulation of primary seed dormancy by MAJOR LATEX PROTEIN-LIKE PROTEIN329 in Arabidopsis is dependent on DNA-BINDING ONE ZINC FINGER6[J]. Journal of Experimental Botany, 2022, 73(19): 6838-6852.
[42]
FUJITA K, INUI H. Review: Biological functions of major latex-like proteins in plants[J]. Plant Science, 2021, 306: 110856.
[43]
KANG Y Y, TONG J L, LIU W, et al. Comprehensive analysis of major latex-like protein family genes in cucumber (Cucumis sativus L.) and their potential roles in Phytophthora blight resistance[J]. International Journal of Molecular Sciences, 2023, 24(1): 784.
[44]
WU J N, KIM S G, KANG K Y, et al. Overexpression of a pathogenesis-related protein 10 enhances biotic and abiotic stress tolerance in rice[J]. The Plant Pathology Journal, 2016, 32(6): 552-562.
[45]
TAKEUCHI K, GYOHDA A, TOMINAGA M, et al. RSOsPR10 expression in response to environmental stresses is regulated antagonistically by jasmonate/ethylene and salicylic acid signaling pathways in rice roots[J]. Plant and Cell Physiology, 2011, 52(9): 1686-1696.
[46]
FUJITA K, ASUKE S, ISONO E, et al. MLP-PG1, a major latex-like protein identified in Cucurbita pepo, confers resistance through the induction of pathogenesis-related genes[J]. Planta, 2022, 255: 10.
[47]
TSUJISHITA Y, HURLEY J H. Structure and lipid transport mechanism of a StAR-related domain[J]. Nature Structural Biology, 2000, 7(5): 408-414.
[48]
FUCHS S, TISCHER S V, WUNSCHEL C, et al. Abscisic acid sensor RCAR7/PYL13, specific regulator of protein phosphatase coreceptors[J]. Proceedings of the National Academy of Sciences of the United States of America, 2014, 111(15): 5741-5746.
[49]
SRIVASTAVA S, FRISTENSKY B, KAV N N V. Constitutive expression of a PR10 protein enhances the germination of Brassica napus under saline conditions[J]. Plant and Cell Physiology, 2004, 45(9): 1320-1324.
[50]
HAN X J, HE X L, QIU W M, et al. Pathogenesis-related protein PR10 from Salix matsudana Koidz exhibits resistance to salt stress in transgenic Arabidopsis thaliana[J]. Environmental and Experimental Botany, 2017, 141: 74-82.
[51]
FENG Y D, REN Y P, ZHANG H, et al. Halostachys caspica pathogenesis-related protein 10 acts as a cytokinin reservoir to regulate plant growth and development[J]. Frontiers in Plant Science, 2023, 14: 1116985.
[52]
LIU H. Functional analysis of NtMLP423 gene in tobacco under drought and chilling stress[D]. Taian: Shandong Agricultural University, 2021. (in Chinese)
Journal of Xinjiang University(Natural Science Edition in Chinese and English)
Pages 73-81
Cite this article:
YU D, WANG Y. Advances on the Biological Function of Major Latex Proteins(MLPs) in Plants. Journal of Xinjiang University(Natural Science Edition in Chinese and English), 2025, 42(1): 73-81. https://doi.org/10.13568/j.cnki.651094.651316.2023.10.23.0001
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