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Original Research | Open Access

Ex vivo differentiation of human bone marrow-derived stem cells into neuronal cell-like lineages

Adeeb Al-Zoubi1,2( ), Feras Altwal3, Farah Khalifeh2, Jamil Hermas4, Ziad Al-Zoubi5, Emad Jafar5, Mohammed El-Khateeb6,7
Department of Surgery, University of Illinois College of Medicine at Peoria, Peoria, IL, USA
Stem Cells of Arabia, Amman, Jordan
Department of Neuroscience, School of Graduate and Postdoctoral Studies, Rosalind Franklin University of Medicine and Science, North Chicago, IL, USA
Stem Cell Division, Al-Yamama Company,
Jordan Orthopedic and Spinal Center,
National Center for Diabetes, Endocrinology and Genetics,
Department of Pathology, Faculty of Medicine, University of Jordan, Amman, Jordan
Show Author Information

Abstract

Background:

Methods to obtain safe and practical populations of stem cells (SCs) at a clinical grade that are able to differentiate into neuronal cell lineages are yet to be developed. In a previous study, we showed that mouse bone marrow-derived SCs (BM-SCs) differentiated into neuronal cell-like lineages when put in a neuronal-like environment, which is a special media supplemented with the necessary growth factors needed for the differentiation of SCs into neuronal cell-like lineages.

Aim:

In this study, we aim to assess the potentials of adult human CD34+ and CD133+ SCs to differentiate into neuronal cell-like lineages ex vivo when placed in a neuronal-like microenvironment.

Methods:

The neuronal-like microenvironment was created by culturing cells in nonhematopoietic expansion media (NHEM) supplemented with growth factors that favor differentiation into neuronal cell lineages (low-affinity nerve growth factor [LNGF], mouse spinal cord extract [mSpE], or both). Cultured cells were assessed for neuronal differentiation by cell morphologies and by expression of GFAP.

Results:

Our results show that culturing unpurified human BM-derived mononuclear cells (hBM-MNCs) in NHEM+LNGF+mSpE did not lead to neuronal differentiation. In contrast, culturing of purified CD34+ hBM-SCs in NHEM+LNGF+mSpE favored their differentiation into astrocyte-like cells, whereas culturing of purified CD133+ hBM-SCs in the same media favored their differentiation into neuronal-like cells. Interestingly, coculturing of CD34+ and CD133+ hBM-SCs in the same media enhanced the differentiation into astrocyte-like cells and neuronal-like cells, in addition to oligodendrocyte-like cells.

Conclusion:

These results suggest that a mixture of purified CD34+ and CD133+ cells may enhance the differentiation into neuronal cell-like lineages and give broader neuronal cell lineages than when each of these cell types is cultured alone. This method opens the window for the utilization of specific populations of hBM-SCs to be delivered in a purified form for the potential treatment of neurodegenerative diseases in the future.

Keywords

cell therapyneurodegenerative diseaseneuronsastrocyesCD34+CD133+MACS

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Journal of Neurorestoratology
Volume 4 Issue 1,
December 2016
Pages 35-44
DOI: 10.2147/JN.S101001
Cite this article:
Al-Zoubi A, Altwal F, Khalifeh F, et al. Ex vivo differentiation of human bone marrow-derived stem cells into neuronal cell-like lineages. Journal of Neurorestoratology, 2016, 4(1): 35-44. https://doi.org/10.2147/JN.S101001

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Published: 27 June 2016
© 2016 The Author(s).

© 2016 Al-Zoubi et al. This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution - Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php).
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