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Original Research | Open Access

Transplantation of mononuclear cells from bone marrow in a rat model of Huntington’s disease

Teresa Serrano1, Paula Pierozan2, Esteban Alberti1, Lisette Blanco1, Karelys de la Cuétara Bernal1, María E González1, Nancy Pavón1, Lourdes Lorigados1, María A Robinson-Agramonte1, Jorge A Bergado1( )
International Center for Neurological Restoration (CIREN), La Habana, Cuba
Department of Biochemistry, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, Porto Alegre, Rio Grande do Sul, Brazil
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Abstract

This article investigates the possible effects of transplantation of mononuclear bone marrow cells (mBMCs) to ameliorate or prevent the behavioral impairments and the cellular damage observed in a quinolinic acid (QA) model of Huntington’s disease. mBMCs were isolated using a standard procedure and implanted within the QA-lesioned striatum. Behavior was explored using motor (beam test) and memory (object recognition and Morris water maze) tests. Morphology was evaluated using conventional histology (cresyl violet), bisbenzimide (to evaluate cell vitality), and immunohystochemistry to identify neurons or glia. mBMC-transplanted animals showed improvements in motor coordination (beam test). Regarding memory, object recognition was significantly improved in transplanted animals, while spatial memory (Morris water maze test) was not severely affected by QA and, therefore, the results after transplantation were significant only in the probe-trial retention test. In samples taken from the animals that participated in the behavioral tests, a preserved morphology of striatal neurons and a reduced glial reaction indicated a possible neuroprotective effect of the transplanted mBMCs. A parallel study confirmed that the transplanted mBMCs have a long survival period (1 year follow-up). The results presented confirm the possibility that mBMC transplantation may be a viable therapeutic option for Huntington’s disease.

Keywords

mononuclear bone marrow cellsHuntington’s diseasequinolinic acidtransplantFluoro-Jade Cglial fibrillary acidic proteinneuronal nuclear marker

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Journal of Neurorestoratology
Volume 4 Issue 1,
December 2016
Pages 95-105
DOI: 10.2147/JN.S118435
Cite this article:
Serrano T, Pierozan P, Alberti E, et al. Transplantation of mononuclear cells from bone marrow in a rat model of Huntington’s disease. Journal of Neurorestoratology, 2016, 4(1): 95-105. https://doi.org/10.2147/JN.S118435

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Published: 13 December 2016
© 2016 The Author(s).

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