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Article | Open Access | Online First

The utiliity of FGF2 in the rapid and high-quality cultivation of hMenSCs and endometrial organoids

Min Wang1,§, Hui Chen2,3,§, Xinyue Feng4, Peiyi Wu1, Tongyan Jin1, Xiao Huang2( ), Jinqiu Feng2( )
Research Center of Neuroscience, College of Medicine, Jiaxing University, Jiaxing 314001, China
Shanghai Key Laboratory of Craniomaxillofacial Development and Diseases, Department of Pediatrics, Shanghai Stomatological Hospital, Fudan University, Shanghai 200433, China
Dental Disease prevention and Treatment Center of Minhang District, Shanghai 201103, China
School of Stomatology, Wannan Medical College, Anhui 241002, China

§ Min Wang and Hui Chen contributed equally to this work.

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Highlights

• Establishment of a rapid and high-quality culture platform for hMenSCs & endometrial organoids

• Gene network regulated by FGF2 in hMenSCs & endometrial organoids

• The divergent effects of FGF2 on hMenSCs & endometrial organoids

Graphical Abstract

Our study explores the unexplored potential of FGF2 in boosting the regenerative capacities of hMenSCs and endometrial organoids. We've devised a robust cultivation method and revealed that FGF2 enhanced hMenSC & endometrial organoid regenerative properties via modulating genes related to growth, proliferation, metabolism, and signaling. FGF2’s dual effects on hMenSCs & organoids hold promise for regenerative medicine & endometrial diseases.

Abstract

Human menstrual blood-derived stem cells (hMenSCs) show promise in regenerative medicine and endometrial disease treatments. Organoids, three-dimensional (3D) tissue replicas, provide accurate disease models. Fibroblast growth factor 2 (FGF2), with established roles in stem cell functions, remains underexplored in its impact on hMenSCs and endometrial organoids. Our study aims to explore these unexplored effects of FGF2. In our study, we have developed a method for rapid and high-quality cultivation of MenSCs and organoids. Using this novel platform, we demonstrate that FGF2 alters hMenSC morphology, enhancing growth and proliferative, migratory, and anti-aging capabilities. RNA sequencing suggests this is linked to downregulated cell adhesion genes. FGF2 promotes proliferation via ribosomal biogenesis and ATP metabolism, possibly delaying senescence. In organoids, FGF2 reverses compaction and volume reduction, promoting growth and fibroblast release. q-PCR analysis shows FGF2 regulates genes related to stemness, proliferation, metabolism, and PI3K-AKT signaling, with distinct patterns in hMenSCs and organoids. This research advances regenerative medicine and reproductive biology by optimizing the cultivation of hMenSCs and organoids with FGF2 for potential therapeutic applications.

Keywords

hMenSCsendometrial organoidsFGF2rapid and high-quality cultivationRNA sequencingregenerative medicine

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Cell Organoid
DOI: 10.26599/CO.2024.9410007
Cite this article:
Wang M, Chen H, Feng X, et al. The utiliity of FGF2 in the rapid and high-quality cultivation of hMenSCs and endometrial organoids. Cell Organoid, 2024, https://doi.org/10.26599/CO.2024.9410007

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Received: 15 March 2024
Revised: 05 July 2024
Accepted: 19 August 2024
Published: 22 October 2024
© The Author(s) 2024. Published by Tsinghua University Press

The articles published in this open access journal are distributed under the termsof the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits use, distribution andreproduction in any medium, provided the original work is properly cited.
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