AI Chat Paper
Discover the SciOpen Platform and Achieve Your Research Goals with Ease.
Search articles, authors, keywords, DOl and etc.
{{expandStatus?'Exit ':''}}Advanced Search
(1) Carvone inhibited the production of virulence factors secreted by S. marcescens.
(2) Carvone enhanced the susceptibility of bacterial/biofilm cells to antibiotics.
(3) Carvone inhibited the expressions of genes involved in virulence, antioxidant enzymes and biofilms.
(4) Carvone enhanced oxidative stress and increased permeability of cell membrane.
Serratia marcescens is a common opportunistic pathogen that causes infections in clinical patients. The aim of this study was to investigate the inhibitory efficiency of carvone on the production of virulence factors and evaluated the susceptibility of antibiotics against biofilms of S. marcescens in combination with carvone. Carvone treatment notably inhibited the production of lipase, prodigiosin, extracellular polysaccharides (EPS), and swimming and swarming motilities. Carvone exposure also reduced the formation of biofilms in S. marcescens NJ01. When exposed to antibiotics in combination with carvone, the inhibitory impact was notably improved. In addition, the expressions of genes glp and katG involved in the synthesis of glutathione peroxidase and catalase, respectively, were significantly suppressed after treatment with carvone. The suppression of antioxidant enzymes resulted in the improvement of reactive oxygen species (ROS) and H2O2, enhanced the permeability of membrane, and eventually increased the susceptibility of biofilms or planktonic cells to antibiotics. The data mentioned above indicated that carvone is expected to play an important role in the control and treatment of S. marcescns infection.
Khayyat, A. N., Abbas, H. A., Khayat, M. T., et al. Secnidazole is a promising imidazole mitigator of Serratia marcescens virulence. Microorganisms, 2021, 9: 2333. https://doi.org/10.3390/microorganisms9112333
Gohil, N., Bhattacharjee, G., Singh, V. Synergistic bactericidal profiling of prodigiosin extracted from Serratia marcescens in combination with antibiotics against pathogenic bacteria. Microbial Pathogenesis, 2020, 149: 104508. https://doi.org/10.1016/j.micpath.2020.104508
Zhou, J. W., Ji, P. C., Wang, C. Y., et al. Synergistic effect of propyl gallate and antibiotics against biofilms of Serratia marcescens and Erwinia carotovora in vitro. LWT-Food Science and Technology, 2023, 173: 114258. https://doi.org/10.1016/j.lwt.2022.114258
Zhou, J. W., Ruan, L. Y., Chen, H. J., et al. Inhibition of quorum sensing and virulence in Serratia marcesscens by hordenine. Journal of Agricultural and Food Chemistry, 2019, 67: 784–795. https://doi.org/10.1021/acs.jafc.8b05922
Aucken, H. M., Pitt, T. L. Antibiotic resistance and putative virulence factors of Serratia marcescens with respect to O and K serotypes. Journal of Medical Microbiology, 1998, 47: 1105–1113. https://doi.org/10.1099/00222615-47-12-1105
Lyerly, D. M., Kreger, A. S. Importance of serratia protease in the pathogenesis of experimental Serratia marcescens pneumonia. Infection and Immunity, 1983, 40: 113–119. https://doi.org/10.1128/iai.40.1.113-119.1983
Salini, R., Pandian, S. K. Interference of quorum sensing in urinary pathogen Serratia marcescens by Anethum graveolens. Pathogens and Disease, 2015, 73: ftv038. https://doi.org/10.1093/femspd/ftv038
Zhou, J. W., Ji, P. C., Wang, C. Y., et al. Anti-virulence activity of dihydrocuminyl aldehyde and nisin against spoilage bacterium Pseudomonas aeruginosa XZ01. LWT-Food Science and Technology, 2023, 177: 114573. https://doi.org/10.1016/j.lwt.2023.114573
Decarvalho, C., Dafonseca, M. Carvone: Why and how should one bother to produce this terpene. Food Chemistry, 2006, 95: 413–422. https://doi.org/10.1016/j.foodchem.2005.01.003
Kanekar, S., Fathima, F., Rekha, P. D. Carvone–a quorum sensing inhibitor blocks biofilm formation in Chromobacterium violaceum. Natural Product Research, 2022, 36: 4540–4545. https://doi.org/10.1080/14786419.2021.1993214
Porfírio, E. M., Melo, H. M., Pereira, A. M. G., et al. In vitro antibacterial and antibiofilm activity of Lippia alba essential oil, citral, and carvone against Staphylococcus aureus. Scientific World Journal, 2017, 2017: 4962707. https://doi.org/10.1155/2017/4962707
Li, T. T., Mei, Y. C., He, B. B., et al. Reducing quorum sensing-mediated virulence factor expression and biofilm formation in Hafnia alvei by using the potential quorum sensing inhibitor L-carvone. Frontiers in Microbiology, 2019, 9: 3324. https://doi.org/10.3389/fmicb.2018.03324
Srinivasan, R., Devi, K. R., Kannappan, A., et al. Piper betle and its bioactive metabolite phytol mitigates quorum sensing mediated virulence factors and biofilm of nosocomial pathogen Serratia marcescens in vitro. Journal of Ethnopharmacology, 2016, 193: 592–603. https://doi.org/10.1016/j.jep.2016.10.017
Kalpana, B. J., Aarthy, S., Pandian, S. K. Antibiofilm activity of α-amylase from Bacillus subtilis S8-18 against biofilm forming human bacterial pathogens. Applied Biochemistry and Biotechnology, 2012, 167: 1778–1794. https://doi.org/10.1007/s12010-011-9526-2
Packiavathy, I. A. S. V., Priya, S., Pandian, S. K., et al. Inhibition of biofilm development of uropathogens by curcumin–An anti-quorum sensing agent from Curcuma longa. Food Chemistry, 2014, 148: 453–460. https://doi.org/10.1016/j.foodchem.2012.08.002
Sethupathy, S., Ananthi, S., Selvaraj, A., et al. Vanillic acid from Actinidia deliciosa impedes virulence in Serratia marcescens by affecting S-layer, flagellin and fatty acid biosynthesis proteins. Scientific Reports, 2017, 7: 16328. https://doi.org/10.1038/s41598-017-16507-x
Babii, O., Afonin, S., Berditsch, M., et al. Controlling biological activity with light: Diarylethene-containing cyclic peptidomimetics. Angewandte Chemie International Edition, 2014, 53: 3392–3395. https://doi.org/10.1002/anie.201310019
Min, L. F., He, S. Y., Chen, Q., et al. Comparative proteomic analysis of cellular response of human airway epithelial cells (A549) to benzo(a)pyrene. Toxicology Mechanisms and Methods, 2011, 21: 374–382. https://doi.org/10.3109/15376516.2010.551555
Zhou, J. W., Muhammad, J., Sun, B., et al. Metabolomic analysis of quorum sensing inhibitor hordenine on Pseudomonas aeruginosa. Applied Microbiology and Biotechnology, 2019, 103: 6271–6285. https://doi.org/10.1007/s00253-019-09878-w
Luo, H. Z., Zhou, J. W., Sun, B., et al. Inhibitory effect of norharmane on Serratia marcescens NJ01 quorum sensing-mediated virulence factors and biofilm formation. Biofouling, 2021, 37: 145–160. https://doi.org/10.1080/08927014.2021.1874942
Tao, Y., Qian, L. H., Xie, J. Effect of chitosan on membrane permeability and cell morphology of Pseudomonas aeruginosa and Staphyloccocus aureus. Carbohydrate Polymers, 2011, 86: 969–974. https://doi.org/10.1016/j.carbpol.2011.05.054
Saxena, S., Punjabi, K., Ahamad, N., et al. Nanotechnology approaches for rapid detection and theranostics of antimicrobial resistant bacterial infections. ACS Biomaterials Science & Engineering, 2022, 8: 2232–2257. https://doi.org/10.1021/acsbiomaterials.1c01516
Qi, Y. H., Ji, P. C., Yin, K. Y., et al. Phloretin inhibits quorum sensing and biofilm formation in Serratia marcescens. Molecules, 2023, 28: 8067. https://doi.org/10.3390/molecules28248067
Ramanathan, S., Ravindran, D., Arunachalam, K., et al. Inhibition of quorum sensing-dependent biofilm and virulence genes expression in environmental pathogen Serratia marcescens by petroselinic acid. Antonie Van Leeuwenhoek, 2018, 111: 501–515. https://doi.org/10.1007/s10482-017-0971-y
Kalivoda, E. J., Stella, N. A., Aston, M. A., et al. Cyclic AMP negatively regulates prodigiosin production by Serratia marcescens. Research in Microbiology, 2010, 161: 158–167. https://doi.org/10.1016/j.resmic.2009.12.004
Padmavathi, A. R., Abinaya, B., Pandian, S. K. Phenol, 2, 4-bis(1, 1-dimethylethyl) of marine bacterial origin inhibits quorum sensing mediated biofilm formation in the uropathogen Serratia marcescens. Biofouling, 2014, 30: 1111–1122. https://doi.org/10.1080/08927014.2014.972386
This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
京公网安备11010802044758号