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Research Article | Open Access | Online First

Cornus officinalis vinegar alters the gut microbiota, regulating lipid droplet changes in nonalcoholic fatty liver disease model mice

Li Cao1,2, Ying Wu1,2,3, Kaiyu Liu1, Ningxian Qi1, Jie Zhang1,2, Shanshan Tie1,3, Xuan Li1,3, Pingping Tian1,2, Shaobin Gu1,2,3( )
College of Food and Bioengineering, Henan University of Science and Technology, Luoyang 471023, China
Henan Engineering Research Center of Food Microbiology, Luoyang 471023, China
National Demonstration Center for Experimental Food Processing and Safety Education, Luoyang 471023, China
Show Author Information

Highlights

(1) Cornus officinalis vinegar improved microbiota composition and enhanced gut microbial diversity that decreased by HFD

(2) Cornus officinalis vinegar improved liver function in HFD-fed mice

(3) C.officinalis vinegar improved histological changes and reduced size and number of lipid droplets in HFD-fed mice

(4) Cornus officinalis vinegar reduced liver steatosis in HFD-fed mice

Graphical Abstract

In Brief

Cornus officinalis vinegar reduced body weight, liver weight and liver steatosis area. Cao et al. show that Cornus officinalis vinegar impacts gut microbiota, Specifically, increased the abundance of known beneficial bacteria Bifidobacterium, Lactobacillus, Faecalibaculum at the level of genus in NAFLD model mice. Cornus officinalis vinegar also decreased the number and the size of hepatocyte with fat vacuoles and the volume and number of LD per cell, and changed expression of PLIN2 and ATGL gene to protect against NAFLD.

Abstract

This study investigated the effect of Cornus officinalis vinegar on gut microbiota and hepatic lipid droplet in nonalcoholic fatty liver disease (NAFLD) model mice. The C57BL/6J male mice were divided into three treatment groups: Control group (CON group) fed with a purified diet; high fat diet (HFD) group and C. officinalis vinegar treatment (CVT) group fed with high fat diet. After 10 weeks, the CVT group was orally administered with C. officinalis vinegar once a day for 6 weeks. We found that C.officinalis vinegar improved gut microbiota, significantly reduced body weight and liver injury of HFD-fed mice. Observation of histopathology and ultrastructure showed that C.officinalis vinegar improved histological changes, reduced size and number of lipid droplets. Additionally, C.officinalis vinegar significantly inhibited the expression of perilipin 2 (PLIN-2) and increased the expression of adipose triglyceride lipase (ATGL). In conclusion, C. officinalis vinegar could exert beneficial effects on NAFLD through modulation of gut microbiota and lipid droplets.

Keywords

gut microbiotanonalcoholic fatty liver diseaselipid dropletsC. officinalis vinegar

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Food & Medicine Homology
DOI: 10.26599/FMH.2024.9420002
Cite this article:
Cao L, Wu Y, Liu K, et al. Cornus officinalis vinegar alters the gut microbiota, regulating lipid droplet changes in nonalcoholic fatty liver disease model mice. Food & Medicine Homology, 2024, https://doi.org/10.26599/FMH.2024.9420002

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Received: 08 April 2024
Revised: 22 April 2024
Accepted: 24 April 2024
Published: 25 May 2024
© National R & D Center for Edible Fungus Processing Technology 2024. Published by Tsinghua University Press.

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
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