Henan Natural Products Biotechnology Co., Ltd., Zhengzhou 450002, China
Henan Academy of Sciences, Zhengzhou 450002, China
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Highlights
(1) Artemisia argyi polysaccharide (AAP) was extracted from the leaves of A. argyi，and the polysaccharide content in AAP was determined to be 86.0%.
(2) AAP downregulated the expression of IκBα, improved intestinal stress state, and inhibited the production of TNF-α, IL-6, IL-17, and IL-1β in intestinal tissues.
(3) The contents of SCFAs (acetic acid, propionic acid, butyric acid) in the colon were increased, the numbers and composition of intestinal flora were changed after AAP intervention.
(4) AAP alleviated intestinal inflammation and intestinal flora dysbiosis in LPS-treated mice, it may play a key role in maintaining the intestinal barrier by regulating intestinal flora.
Graphical Abstract
Artemisia argyi polysaccharide (AAP) is an important active component extracted from the leaves of Artemisia argyi (A. argyi). The mice was injected intraperitoneally with LPS to induce intestinal inflammation and barrier injury, and mice in experimental group were gavaged with AAP for the entire experimental period.The study showed that AAP can downregulate the expression of IκBα, improve intestinal stress state, and inhibite the production of TNF-α, IL-6, IL-17, and IL-1β in intestinal tissues.The contents of SCFAs (acetic acid, propionic acid, butyric acid) in the colon were increased, the numbers and composition of intestinal flora were changed after AAP intervention.Results showed that AAP alleviated intestinal inflammation and intestinal flora dysbiosis in LPS-treated mice, it may play a key role in maintaining the intestinal barrier by regulating intestinal flora.
Abstract
Artemisia argyi polysaccharide (AAP) is an important active component extracted from the leaves of A. argyi. Currently, the effects of AAP in mouse model of intestinal inflammation are unclear. At the same time, it is uncertain whether AAP affects the intestinal flora. The study aimed to explore the improvement effects of AAP on lipopolysaccharide (LPS)-treated mice. The changes of intestinal tissue was observed with hematoxylin-eosin (HE) staining, also the mRNA expression of the intestinal barrier-related junction proteins were detected. The intestinal oxidation indices and inflammatory factors were detected with ELISA method, the nuclear factor kappa B (NF-κB) signaling pathway expression was evaluated with Western blotting, and the contents of short-chain fatty acids (SCFAs) in the colon were determined. At the same time, the changes of AAP on intestinal flora were evaluated. The study have shown that AAP downregulated the expression of NF-κB inhibitor protein α (IκBα), improved intestinal stress state, and inhibited the production of tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), interleukin-17 (IL-17), and interleukin-1β (IL-1β) in intestinal tissues. The contents of SCFAs (acetic acid, propionic acid, butyric acid) in the colon were increased, the numbers and composition of intestinal flora were changed after AAP intervention. In conclusion: AAP alleviated intestinal inflammation and intestinal flora dysbiosis in LPS-treated mice, it may play a key role in maintaining the intestinal barrier by regulating intestinal flora.
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References
[1]
Ng, S. C., Shi, H. Y., Hamidi, N., et al. Worldwide incidence and prevalence of inflammatory bowel disease in the 21st century: a systematic review of population-based studies. The Lancet, 2017, 390: 2769–2778. https://doi.org/10.1016/S0140-6736(17)32448-0
Okumura, R., Takeda, K. Roles of intestinal epithelial cells in the maintenance of gut homeostasis. Experimental & Molecular Medicine, 2017, 49: e338. https://doi.org/10.1038/emm.2017.20
Liang, Q., Zhao, Q., Hao, X., et al. The Effect of Flammulina velutipes polysaccharide on immunization analyzed by intestinal flora and proteomics. Frontiers in Nutrition, 2022, 9: 841230. https://doi.org/10.3389/fnut.2022.841230
Yu, Y., Mendoza, T. M., Ribnicky, D. M., et al. An extract of Russian tarragon prevents obesity-related ectopic lipid accumulation. Molecular Nutrition & Food Research, 2018, 62: e1700856. https://doi.org/10.1002/mnfr.201700856
Li, S., Zhou, S., Yang, W., et al. Gastro-protective effect of edible plant Artemisia argyi in ethanol-induced rats via normalizing inflammatory responses and oxidative stress. Journal of Ethnopharmacology, 2018, 214: 207–217. https://doi.org/10.1016/j.jep.2017.12.023
Cui, D., Ma, J., Liang, T., et al. Selenium nanoparticles fabricated in laminarin polysaccharides solutions exert their cytotoxicities in HepG2 cells by inhibiting autophagy and promoting apoptosis. International Journal of Biological Macromolecules, 2019, 137: 829–835. https://doi.org/10.1016/j.ijbiomac.2019.07.031
Chen, S., Wang, J., Fang, Q., et al. A polysaccharide from natural Cordyceps sinensis regulates the intestinal immunity and gut microbiota in mice with cyclophosphamide-induced intestinal injury. Food & Function, 2021, 12: 6271–6282. https://doi.org/10.1039/d1fo00596k
Zhang, P., Sun, D., Shi, B., et al. Dietary supplementation with Artemisia argyi extract on inflammatory mediators and antioxidant capacity in broilers challenged with lipopolysaccharide. Italian Journal of Animal Science, 2020, 19: 1091–1098. https://doi.org/10.1080/1828051X.2020.1816506
Zhang, L., Xing, Y., Shi, L., et al. The effects of dietary supplementation of Artemisia argyi polysaccharide on immune and antioxidative functions in broilers. Journal of Applied Animal Research, 2022, 50: 587–597. https://doi.org/10.1080/09712119.2022.2119982
Jin, M., Zhu, Y., Shao, D., et al. Effects of polysaccharide from mycelia of Ganoderma lucidum on intestinal barrier functions of rats. International Journal of Biological Macromolecules, 2017, 94: 1–9. https://doi.org/10.1016/j.ijbiomac.2016.09.099
Bian, M., Wang, J., Wang, Y., et al. Chicory ameliorates hyperuricemia via modulating gut microbiota and alleviating LPS/TLR4 axis in quail. Biomedicine & Pharmacotherapy, 2020, 131: 110719. https://doi.org/10.1016/j.biopha.2020.110719
Lan, M. B., Zhang, Y. H., Zheng, Y., et al. Antioxidant and immunomodulatory activities of polysaccharides from moxa ( Artemisia argyi) leaf. Food Science and Biotechnology, 2010, 19: 1463–1469. https://doi.org/10.1007/s10068-010-0209-5
Wang, Y. J., Chen, Y., Zhang, X. Y., et al. New insights in intestinal oxidative stress damage and the health intervention effects of nutrients: a review. Journal of Functional Foods, 2020, 75: 104248. https://doi.org/10.1016/j.jff.2020.104248
He, Y., Xu, M., Lu, S., et al. Seaweed polysaccharides treatment alleviates injury of inflammatory responses and gut barrier in LPS-induced mice. Microbial Pathogenesis, 2023, 180: 106159. https://doi.org/10.1016/j.micpath.2023.106159
Li, R. L., Zhao, D. Y., Li, P. P., et al. Effects of thymol and rosmarinic acid combination on growth performance, inflammatory response and intestinal health of lipopolysaccharide challenged rats. Chinese Journal of Animal Nutrition, 2022, 34: 2009–2022. https://doi.org/10.3969/j.issn.1006-267x.2022.03.059
Yin, L., Zhou, J., Chen, D., et al. A review of the application of near-infrared spectroscopy to rare traditional Chinese medicine. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 2019, 221: 117208. https://doi.org/10.1016/j.saa.2019.117208
Ji, X., Liu, F., Peng, Q., et al. Purification, structural characterization, and hypolipidemic effects of a neutral polysaccharide from Ziziphus Jujuba cv. Muzao. Food Chemistry, 2018, 245: 1124–1130. https://doi.org/10.1016/j.foodchem.2017. 11.058
Geng, L., Hu, W., Liu, Y., et al. A heteropolysaccharide from Saccharina japonica with immunomodulatory effect on RAW 264.7 cells. Carbohydrate Polymers, 2018, 201: 557–565. https://doi.org/10.1016/j.carbpol.2018.08.096
Saia, R. S., Ribeiro, A. B., Giusti H. Cholecystokinin modulates the mucosal inflammatory response and prevents the lipopolysaccharide-induced intestinal epithelial barrier dysfunction. Shock, 2020, 53: 242–251. https://doi.org/10.1097/SHK.0000000000001355
Luo, J. Y., Li, Y. H., Fang, B. B., et al. Gene rs28362491 ins/del variation is associated with higher susceptibility to myocardial infarction in a Chinese Han population. Scientific Reports, 2020, 10: 19518. https://doi.org/10.1038/s41598-020-72877-9
Koh, A., de Vadder, F., Kovatcheva-Datchary P., et al. From dietary fiber to host physiology: short-chain fatty acids as key bacterial metabolites. Cell, 2016, 165: 1332–1345. https://doi.org/10.1016/j.cell.2016.05.041
Li, X., Chen, S., Zhao, Z. T., et al. Effects of polysaccharides from Yingshan Yunwu tea on meat quality, immune status and intestinal microflora in chickens. International Journal of Biological Macromolecules, 2020, 155: 61–70. https://doi.org/10.1016/j.ijbiomac.2020.03.198
Wang, Y., Song, X., Wang, Z., et al. Effects of pine pollen polysaccharides and sulfated polysaccharides on ulcerative colitis and gut flora in mice. Polymers, 2023, 15: 1414. https://doi.org/10.3390/polym15061414
Sanjeewa, K. K. A., Nagahawatta, D. P., Yang, H. W., et al. Octominin inhibits LPS-induced chemokine and pro-inflammatory cytokine secretion from RAW264.7 macrophages via blocking TLRs/NF-κB signal transduction. Biomolecules, 2020, 10: 511. https://doi.org/10.3390/biom10040511
Wu, Y. R., Kuang, G. Y., Lu, F. G., et al. Pathological relationship between intestinal flora and osteoarthritis and intervention mechanism of Chinese medicine. Chinese Journal of Integrative Medicine, 2019, 25: 716–720. https://doi.org/10.1007/s11655-019-3224-2
Li, S., Zhuge, A., Wang, K., et al. Ketogenic diet aggravates colitis, impairs intestinal barrier and alters gut microbiota and metabolism in DSS-induced mice. Food & Function, 2021, 12: 10210–10225. https://doi.org/10.1039/d1fo02288a
Kaminsky, L. W., Al-Sadi, R., Ma, T. Y. IL-1β and the intestinal epithelial tight junction barrier. Frontiers in Immunology, 2021, 12: 767456. https://doi.org/10.3389/fimmu.2021.767456
Chen, M., Xiao, D., Liu, W., et al. Intake of Ganoderma lucidum polysaccharides reverses the disturbed gut microbiota and metabolism in type 2 diabetic rats. International Journal of Biological Macromolecules, 2020, 155: 890–902. https://doi.org/10.1016/j.ijbiomac.2019.11.047
Yin, Z., Liang, Z., Li, C., et al. Immunomodulatory effects of polysaccharides from edible fungus: a review. Food Science and Human Wellness, 2021, 10: 393–400. https://doi.org/10.1016/j.fshw.2021.04.001
Niu, Y., Dong, J., Jiang, H., et al. Effects of polysaccharide from Malus halliana Koehne flowers in cyclophosphamide-induced immunosuppression and oxidative stress on mice. Oxidative Medicine and Cellular Longevity, 2020, 2020: 1603735. https://doi.org/10.1155/2020/1603735
Akdis, C. A. Does the epithelial barrier hypothesis explain the increase in allergy, autoimmunity and other chronic conditions? Nature Reviews Immunology, 2021 , 21: 739−751. https://doi.org/10.1038/s41577-021-00538-7
Wang, J., Liang, Q., Zhao, Q., et al. The effect of microbial composition and proteomic on improvement of functional constipation by Chrysanthemum morifolium polysaccharide. Food and Chemical Toxicology, 2021, 153: 112305. https://doi.org/10.1016/j.fct.2021.112305
Lai, L., Chen, Y., Tian, X., et al. Artesunate alleviates hepatic fibrosis induced by multiple pathogenic factors and inflammation through the inhibition of LPS/TLR4/NF-κB signaling pathway in rats. European Journal of Pharmacology, 2015, 765: 234–241. https://doi.org/10.1016/j.ejphar.2015.08.040
Zheng, J., Ahmad, A. A., Yang, C., et al. Orally administered Lactobacillus rhamnosus CY12 alleviates DSS-induced colitis in mice by restoring the intestinal barrier and inhibiting the TLR4-MyD88-NF-κB pathway via intestinal microbiota modulation. Journal of Agricultural and Food Chemistry, 2024, 72: 9102–9166. https://doi.org/10.1021/acs.jafc.3c07279
Liu, C., Cheng, L., Ji, L., et al. Intestinal microbiota dysbiosis play a role in pathogenesis of patients with primary immune thrombocytopenia. Thrombosis Research, 2020, 190: 11–19. https://doi.org/10.1016/j.thromres.2020.03.012
Zhang, J., Zhong, Y., Wang, D., et al. Wallace melon juice fermented with Lactobacillus alleviates dextran sulfate sodium-induced ulcerative colitis in mice through modulating gut microbiota and the metabolism. Journal of Food Science, 2024, 89: 2450–2464. https://doi.org/10.1111/1750-3841.16973
Ning E-J, Sun C-W, Wang X-F, et al. Artemisia argyi polysaccharide alleviates intestinal inflammation and intestinal flora dysbiosis in lipopolysaccharide-treated mice. Food & Medicine Homology, 2024, 1(1): 9420008. https://doi.org/10.26599/FMH.2024.9420008
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