Henan Natural Products Biotechnology Co., Ltd., Zhengzhou 450002, China
Henan Academy of Sciences, Zhengzhou 450002, China
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Highlights
(1) Artemisia argyi polysaccharide (AAP) was extracted from the leaves of A. argyi，and the polysaccharide content in AAP was determined to be 86.0%.
(2) AAP downregulated the expression of IκBα, improved intestinal stress state, and inhibited the production of TNF-α, IL-6, IL-17, and IL-1β in intestinal tissues.
(3) The contents of SCFAs (acetic acid, propionic acid, butyric acid) in the colon were increased, the numbers and composition of intestinal flora were changed after AAP intervention.
(4) AAP alleviated intestinal inflammation and intestinal flora dysbiosis in LPS-treated mice, it may play a key role in maintaining the intestinal barrier by regulating intestinal flora.
Graphical Abstract
Artemisia argyi polysaccharide (AAP) is an important active component extracted from the leaves of Artemisia argyi (A. argyi). The mice was injected intraperitoneally with LPS to induce intestinal inflammation and barrier injury, and mice in experimental group were gavaged with AAP for the entire experimental period.The study showed that AAP can downregulate the expression of IκBα, improve intestinal stress state, and inhibite the production of TNF-α, IL-6, IL-17, and IL-1β in intestinal tissues.The contents of SCFAs (acetic acid, propionic acid, butyric acid) in the colon were increased, the numbers and composition of intestinal flora were changed after AAP intervention.Results showed that AAP alleviated intestinal inflammation and intestinal flora dysbiosis in LPS-treated mice, it may play a key role in maintaining the intestinal barrier by regulating intestinal flora.
Abstract
Artemisia argyi polysaccharide (AAP) is an important active component extracted from the leaves of A. argyi. Currently, the effects of AAP in mouse model of intestinal inflammation are unclear. At the same time, it is uncertain whether AAP affects the intestinal flora. The study aimed to explore the improvement effects of AAP on lipopolysaccharide (LPS)-treated mice. The changes of intestinal tissue was observed with hematoxylin-eosin (HE) staining, also the mRNA expression of the intestinal barrier-related junction proteins were detected. The intestinal oxidation indices and inflammatory factors were detected with ELISA method, the nuclear factor kappa B (NF-κB) signaling pathway expression was evaluated with Western blotting, and the contents of short-chain fatty acids (SCFAs) in the colon were determined. At the same time, the changes of AAP on intestinal flora were evaluated. The study have shown that AAP downregulated the expression of NF-κB inhibitor protein α (IκBα), improved intestinal stress state, and inhibited the production of tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), interleukin-17 (IL-17), and interleukin-1β (IL-1β) in intestinal tissues. The contents of SCFAs (acetic acid, propionic acid, butyric acid) in the colon were increased, the numbers and composition of intestinal flora were changed after AAP intervention. In conclusion: AAP alleviated intestinal inflammation and intestinal flora dysbiosis in LPS-treated mice, it may play a key role in maintaining the intestinal barrier by regulating intestinal flora.
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