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Research Article | Open Access

The anti-aging effect of a nutritional supplement based on Lycopene and grape extract

Yao Xie2, Zi-Jia Lu2, Li-Bo Du3, Qian-Yi Huang2, A-Xin Liang1()
School of Life Science, Beijing Institute of Technology, Beijing 100081, China
Dongfanghong Aerospace Biotech Co. Ltd., Beijing 100190, China
Structural Key Laboratory for Unstable and Stable Species, Institute of Chemistry Chinese Academy of Sciences, Beijing 100190, China
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Highlights

(1) Exploring the anti-aging effects of a nutritional supplement (lycopene/grape extract = 1.5:1).

(2) Examined the mean lifespan, athletic and reproductive abilities, the levels of lipofuscin and reactive oxygen species (ROS) in Caenorhabditis elegans (C. elegans).

(3) Discussed the potential mechanism of the nutritional supplement (lycopene/grape extract = 1:1) on anti-aging.

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This study examined the anti-aging effects of a nutritional supplement (lycopene/grape extract=1.5:1). The mean lifespan, athletic ability, reproductive ability, the levels of lipofuscin, reactive oxygen species (ROS), and several critical genes related to lifespan regulation in Caenorhabditis elegans (C. elegans) were examined and determined. According to the results, the nutritional supplement effectively extended the mean lifespan and improved the abilities of athletic and reproductive of C. elegans dose-dependently. Meanwhile, the levels of lipofuscin and ROS decreased significantly in the three dosage groups (P < 0.05). Furthermore, the nutritional supplement upregulated the mRNA expression of SOD3, GST4 (P < 0.01), and HSP16.2 (P < 0.05) genes. Our findings showed the nutritional supplement could prolong the lifespan of C. elegans through reducing the levels of lipofuscin and ROS, and enhance the resistance against oxidative stress.

Abstract

The demand for healthcare products is growing worldwide beyond the safety and convenience. This study examined the anti-aging effects of a nutritional supplement (lycopene/grape extract = 1.5:1), which is a healthcare product. The mean lifespan, athletic and reproductive abilities were examined in Caenorhabditis elegans (C. elegans). Additionally, the levels of lipofuscin, reactive oxygen species (ROS), and several critical genes related to lifespan regulation in C. elegans were determined. According to the results, the nutritional supplement effectively extended the mean lifespan and improved the abilities of athletic and reproductive of C. elegans dose-dependently. Meanwhile, the levels of lipofuscin and ROS decreased significantly in the three dosage groups (P < 0.05). Furthermore, the nutritional supplement upregulated the mRNA expression of SOD3, GST4 (P < 0.01), and HSP16.2 (P < 0.05) genes. Our findings showed the nutritional supplement could prolong the lifespan of C. elegans through reducing the levels of lipofuscin and ROS, and enhance the resistance against oxidative stress.

Keywords

nutritional supplementCaenorhabditis elegansantioxidantsanti-agingreactive oxygen species

References

[1]

Lopez-Otin, C., Blasco, M. A., Partridge, L, et al. The hallmarks of aging. Cell, 2013, 153: 1194–1217. https://doi.org/10.1016/j.cell.2013.05.039

Crossref Google Scholar
[2]

Gladyshev, V. N., Kritchevsky, S. B., Clarke, S. G., et al. Molecular damage in aging. Nature Aging, 2021, 1: 1096–1106. https://doi.org/10.1038/s43587-021-00150-3

Crossref Google Scholar
[3]

Kowald, A., Passos, J. F., Kirkwood, T. B. L. On the evolution of cellular senescence. Aging Cell, 2020, 19: e13270. https://doi.org/10.1111/acel.13270

Crossref Google Scholar
[4]

Long, E., Zhang, J. Z. Evidence for the role of selection for reproductively advantageous alleles in human aging. Science Advances, 2023, 9: eadh4990. https://doi.org/10.1126/sciadv.adh4990

Crossref Google Scholar
[5]

Roberts, S. B., Rosenberg, I. Nutrition and aging: changes in the regulation of energy metabolism with aging. Physiological Reviews, 2006, 86: 651–667. https://doi.org/10.1152/physrev.00019.2005

Crossref Google Scholar
[6]

Sies, H., Berndt, C., Jones, D. P. Oxidative Stress. Annual Review of Biochemistry, 2017, 86: 715–748. https://doi.org/10.1146/annurev-biochem-061516-045037

Crossref Google Scholar
[7]

Hui, H., Xin, A., Cui, H. Y., et al. Anti-aging effects on Caenorhabditis elegans of a polysaccharide, O-acetyl glucomannan, from roots of Lilium davidii var. unicolor Cotton. International journal of biological macromolecules, 2020, 155: 846–852. https://doi.org/10.1016/j.ijbiomac.2020.03.206

Crossref Google Scholar
[8]

Sohal, R. S., Weindruch, R. Oxidative Stress, Caloric Restriction, and Aging. Science, 1996, 273: 59–63. https://doi.org/10.1126/science.273.5271.59

Crossref Google Scholar
[9]

Mossad, O., Batut, B., Yilmaz, B., et al. Gut microbiota drives age-related oxidative stress and mitochondrial damage in microglia via the metabolite N6-carboxymethyllysine. Nature Neuroscience, 2022, 25: 295–305. https://doi.org/10.1038/s41593-022-01027-3

Crossref Google Scholar
[10]

Hu, Q. B. , Hu, S. Q., Fleming, E., et al. Chitosan-caseinate-dextran ternary complex nanoparticles for potential oral delivery of astaxanthin with significantly improved bioactivity. International journal of biological macromolecules, 2020, 151: 747–756. https://doi.org/10.1016/j.ijbiomac.2020.02.170

Crossref Google Scholar
[11]

Jiang, X., Liu, X. Y., Yu, Q., et al. Functional resveratrol-biodegradable manganese doped silica nanoparticles for the spinal cord injury treatment. Materials Today Bio, 2022, 13: 100177. https://doi.org/10.1016/j.mtbio.2021.100177

Crossref Google Scholar
[12]

Liang, X. P., Ma, C. C., Yan, X. J., et al. Advances in research on bioactivity, metabolism, stability and delivery systems of lycopene. Trends in Food Science & Technology, 2019, 93: 185–196. https://doi.org/10.1016/j.jpgs.2019.08.019

Crossref Google Scholar
[13]

Yuan, R., Li, Y. Q., Han, S., et al. Fe-Curcumin nanozyme-mediated reactive oxygen species scavenging and anti-inflammation for acute lung injury. ACS Central Science, 2022, 8: 10–21. https://doi.org/10.1021/acscentsci.1c00866

Crossref Google Scholar
[14]

Wang, X., Falcone, T., Attaran, M., et al. Vitamin C and vitamin E supplementation reduce oxidative stress–induced embryo toxicity and improve the blastocyst development rate. Fertility and Sterility, 2002, 78: 1272–1277. https://doi.org/10.1016/S0015-0282(02)04236-X

Crossref Google Scholar
[15]

Zhu, X., Li, Z., Li, C., et al. Ginkgo biloba extract and Aspirin synergistically attenuate activated platelet-induced ROS production and LOX-1 expression in human coronary artery endothelial cells. Phytomedicine, 2013, 20: 114–119. https://doi.org/10.1016/j.phymed.2012.10.005

Crossref Google Scholar
[16]

Wang, T., Fan, Q., Hong, J., et al. Therapeutic Nanoparticles from grape seed for modulating oxidative stress. Small, 2021, 17: 2102485. https://doi.org/10.1002/smll.202102485

Crossref Google Scholar
[17]

Grabowska, M., Wawrzyniak, D., Rolle, K., et al. Let food be your medicine: Nutraceutical properties of lycopene. Food & Function, 2019, 10: 3090–3102. https://doi.org/10.1039/C9FO00580C

Crossref Google Scholar
[18]

Restani, P., Fradera, U., Ruf, J. C., et al. Grapes and their derivatives in modulation of cognitive decline: A critical review of epidemiological and randomized-controlled trials in humans. Critical Reviews in Food Science and Nutrition, 2021, 61: 566–576. https://doi.org/10.1080/10408398.2020.1740644

Crossref Google Scholar
[19]

Zhu, J., Du, C. Could grape-based food supplements prevent the development of chronic kidney disease. Critical Reviews in Food Science and Nutrition, 2020, 60: 3054–3062. https://doi.org/10.1080/10408398.2019.1676195

Crossref Google Scholar
[20]

Lin, Y., Lin, C., Cao, Y., et al. Caenorhabditis elegans as an in vivo model for the identification of natural antioxidants with anti-aging actions. Biomedicine & Pharmacotherapy, 2023, 167: 115594. https://doi.org/10.1016/j.biopha.2023.115594

Crossref Google Scholar
[21]

Flatt, T., Partridge, L. Horizons in the evolution of aging. BMC Biology, 2018, 16: 93. https://doi.org/10.1186/s12915-018-0562-z

Crossref Google Scholar
[22]

Li, R., Yi, Q., Wang, J., et al. Paeonol promotes longevity and fitness in Caenorhabditis elegans through activating the DAF-16/FOXO and SKN-1/Nrf2 transcription factors. Biomedicine & Pharmacotherapy, 2024, 173: 116368. https://doi.org/10.1016/j.biopha.2024.116368

Crossref Google Scholar
[23]

Magalhães, J. P. D., Wuttke, D., Wood, S. H., et al. Genome-environment interactions that modulate aging: powerful targets for drug discovery. Pharmacological Reviews, 2012, 64: 88–101. https://doi.org/10.1124/pr.110.004499

Crossref Google Scholar
[24]

Gjorgjieva, J., Biron, D., Haspel, G. Neurobiology of caenorhabditis elegans locomotion: where do we stand. BioScience, 2014, 64: 476–486. https://doi.org/10.1093/biosci/biu058

Crossref Google Scholar
[25]

Gusarov, I., Shamovsky, I., Pani, B., et al. Dietary thiols accelerate aging of C. elegans. Nature Communications, 2021, 12: 4336. https://doi.org/10.1038/s41467-021-24634-3

Crossref Google Scholar
[26]

Mao, K., Breen, P., Ruvkun, G. Mitochondrial dysfunction induces RNA interference in C. elegans through a pathway homologous to the mammalian RIG-I antiviral response. PLOS Biology, 2020, 18: e3000996. https://doi.org/10.1371/journal.pbio.3000996

Crossref Google Scholar
[27]

Pinkston, J. M., Garigan, D., Hansen, M., et al. Mutations that increase the life span of C. elegans inhibit tumor growth. Science, 2006, 313: 971–975. https://doi.org/10.1126/science.1121908

Crossref Google Scholar
[28]

Wahba, L., Hansen, L., Fire, A. Z. An essential role for the piRNA pathway in regulating the ribosomal RNA pool in C. elegans. Developmental Cell, 2021, 56: 2295–2312.e6. https://doi.org/10.1016/j.devcel.2021.07.014

Crossref Google Scholar
[29]

Zhang, J., Dong,S., Zhao, S.H., et al. Anti-aging effects of Lycium ruthenicum Murr. Granules in Caenorhabditis elegans. Pakistan journal of zoology, 2020, 52: 1061–1069. https://doi.org/10.17582/journal.pjz/20191016031055

Crossref Google Scholar
[30]

Stiernagle, T. Maintenance of C. elegans. WormBook, 2006, 11: 1–11. https://doi.org/10.1895/wormbook.1.101.1

Crossref Google Scholar
[31]

Ding, C., Feng, S., Fu, L., et al. Heat stress resistance effect of flavonoids from Penthorum chinense Pursh on Caenorhabditis elegans. Pharmacognosy Magazine, 2019, 15: 514–514. https://doi.org/10.4103/pm.pm_500_18

Crossref Google Scholar
[32]

Horst, A. M., Vukanti, R., Priester, J. H. et al. An assessment of fluorescence- and absorbance-based assays to study metal-oxide nanoparticle ROS production and effects on bacterial membranes. Small, 2013, 9: 1753–1764. https://doi.org/10.1002/smll.201201455

Crossref Google Scholar
[33]

Wang, J., Deng, N., Wang, H., et al. Effects of orange extracts on longevity, healthspan, and stress resistance in Caenorhabditis elegans. Molecules, 2020, 25: 351. https://doi.org/10.3390/molecules25020351

Crossref Google Scholar
[34]

Wan, R., Wang, Z. W., Li, H., et al. Human leukocyte antigen-g inhibits the anti-tumor effect of natural killer cells via immunoglobulin-like transcript 2 in gastric cancer. Cellular physiology and biochemistry, 2017, 44: 1828–1841. https://doi.org/10.1159/000485819

Crossref Google Scholar
[35]

Wang, X., Wang, X., Li, L., et al. Lifespan extension in Caenorhabditis elegans by DMSO is dependent on sir-2.1 and daf-16. Biochemical and biophysical research communications, 2010, 400: 613–618. https://doi.org/10.1016/j.bbrc.2010.08.113

Crossref Google Scholar
[36]

Guglielmi, L., Cinnella, C., Nardella, M., et al. MYCN gene expression is required for the onset of the differentiation programme in neuroblastoma cells. Cell Death & Disease, 2014, 5: e1081–e1081. https://doi.org/10.1038/cddis.2014.42

Crossref Google Scholar
[37]

Li, N., Li, Q., He, X., et al. Antioxidant and anti-aging activities of Laminaria japonica polysaccharide in Caenorhabditis elegans based on metabonomic analysis. International Journal of Biological Macromolecules, 2022, 221: 346–354. https://doi.org/10.1016/j.ijbiomac.2022.09.008

Crossref Google Scholar
[38]

Ahmadian, E., Eftekhari, A., Kavetskyy, T., et al. Effects of quercetin loaded nanostructured lipid carriers on the paraquat-induced toxicity in human lymphocytes. Pesticide biochemistry and physiology, 2020, 167: 104586. https://doi.org/10.1016/j.pestbp.2020.104586

Crossref Google Scholar
[39]

Galkin, F., Zhang, B., Dmitriev, S. E., et al. Reversibility of irreversible aging. Ageing research reviews, 2019, 49: 104–114. https://doi.org/10.1016/j.arr.2018.11.008

Crossref Google Scholar
[40]

Longo, V. D., Anderson, R. M. Nutrition, longevity and disease: from molecular mechanisms to interventions. Cell, 2022, 185: 1455–1470. https://doi.org/10.1016/j.cell.2022.04.002

Crossref Google Scholar
Food & Medicine Homology
Volume 2 Issue 2,
June 2025
Article number: 9420037
DOI: 10.26599/FMH.2025.9420037
Cite this article:
Xie Y, Lu Z-J, Du L-B, et al. The anti-aging effect of a nutritional supplement based on Lycopene and grape extract. Food & Medicine Homology, 2025, 2(2): 9420037. https://doi.org/10.26599/FMH.2025.9420037
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