AI Chat Paper
Note: Please note that the following content is generated by AMiner AI. SciOpen does not take any responsibility related to this content.
{{lang === 'zh_CN' ? '文章概述' : 'Summary'}}
{{lang === 'en_US' ? '中' : 'Eng'}}
Chat more with AI
Powered by AMiner. Clicking this button will take you away from SciOpen.
Home Food Science of Animal Products Article
PDF (958.7 KB)
Cite
EndNote(RIS) BibTeX
Collect
Submit Manuscript AI Chat Paper
Show Outline
Outline
Show full outline
Hide outline
Outline
Show full outline
Hide outline
Research Article | Open Access

Association of polymorphisms in IGF2, CLU and STAT5A genes with milk production characteristics in Chinese Holstein cattle

Shangchen Fu, Ting Ku, Linqiang Li, Yufang Liu( ), Yongfeng Liu( )
School of Food Engineering and Nutritional Science, Shaanxi Normal University, Xi’an 710062, China
Show Author Information

Abstract

Reflecting the quality of milk at the molecular level is a frontier technology. The aim of this study was to analyze the polymorphisms of bovine insulin-like growth factor 2 (IGF2), signal transducer and activator of transcription 5A (STAT5A) and clusterin (CLU) genes in the raw milk from 507 Chinese Holstein cow using polymerase chain reaction (PCR)-restriction fragment length polymorphism techniques and to evaluate their correlations with the milk protein content (MPC), milk fat content (MFC), milk lactose content (MLC) and milk total solids content (MTSC). In IGF2 gene, genotype GG was the most frequent genotype (51.68%) followed by the genotype GT (38.03%) and TT (10.29%). And the genotype TT of IGF2 gene was superior to the other genotypes in MPC. In CLU gene, genotype GG was the most common genotype (63.99%) followed by the genotype GA (34.45%) and AA (1.56%). And the genotype AA of CLU gene had greater MFC and MLC, but lower MTSC than GA genotype individuals. For STAT5A gene, the frequency of genotype CC and CT was similar (45.30% and 45.08%), while the genotype TT had lowest frequency (9.62%). And the genotype TT of STA5A gene had highest MPC and lowest MLC. Thus, screening for the IGF2, CLU and STAT5A genes were available for evaluating milk quality and raw milk samples were graded according to the different genotypes.

Keywords

milk qualitySTAT5A geneCLU geneIGF2 genemutation

References

[1]

D. Porcellato, M. Aspholm, S. B. Skeie, et al., Microbial diversity of consumption milk during processing and storage, Int. J. Food Microbiol. 266 (2018) 21–30. https://doi.org/10.1016/j.ijfoodmicro.2017.11.004.
Crossref Google Scholar
[2]

D. Houle, D. R. Govindaraju, S. Omholt, Phenomics: the next challenge, Nat. Rev. Genet. 11(12) (2010) 855–866. https://doi.org/10.1038/nrg2897.
Crossref Google Scholar
[3]

N. S. Yudin, M. I. Voevoda, Molecular genetic markers of economically important traits in dairy cattle, Russ. J. Genet. 51(5) (2015) 600–612. https://doi.org/10.1134/S1022795415050087.
Crossref Google Scholar
[4]

Z. Wang, T. Li, W. Yu, et al., Determination of content of camel milk in adulterated milk samples by normalized real-time polymerase chain reaction system based on single-copy nuclear genes, J. Sci. Food Agr. 100(8) (2020) 3465–3470. https://doi.org/10.1002/jsfa.10382.
Crossref Google Scholar
[5]

M. G. Pizarro Inostroza, F. J. Navas González, J. M. León Jurado, et al., Bayesian evaluation of the effect of non-genetic factors on the phenomics for quality-related milk nutrients and yield in Murciano-Granadina goats, Trop. Anim. Health Pro. 54(6) (2022) 388. https://doi.org/10.1007/s11250-022-03385-3.
Crossref Google Scholar
[6]

K. Flisikowski, A. Maj, L. Zwierzchowski, et al., Nucleotide sequence and variation of IGF2 gene exon 6 in Bos taurus and Bos indicus cattle, Anim. Biotechnol. 16(2) (2005) 203–208. https://doi.org/10.1080/10495390500278060.
Crossref Google Scholar
[7]

X. He, M. X. Chu, L. Qiao, et al., Polymorphisms of STAT5A gene and their association with milk production traits in Holstein cows, Mol. Biol. Rep. 39(3) (2012) 2901–2907. https://doi.org/10.1007/s11033-011-1051-4.
Crossref Google Scholar
[8]

C. Li, W. Cai, C. Zhou, et al., RNA-Seq reveals 10 novel promising candidate genes affecting milk protein concentration in the Chinese Holstein population, Sci. Rep. 6(1) (2016) 26813. https://doi.org/10.1038/srep26813.
Crossref Google Scholar
[9]

L. Yan, X. Fang, Y. Liu, et al., Exploring the genetic variants of insulin-like growth factor II gene and their associations with two production traits in Langshan chicken, J. Appl. Anim. Res. 45(1) (2017) 60–63. https://doi.org/10.1080/09712119.2015.1124328.
Crossref Google Scholar
[10]

L. A. Vaccaro, T. E. Porter, L. E. Ellestad, The effect of commercial genetic selection on somatotropic gene expression in broilers: a potential role for insulin-like growth factor binding proteins in regulating broiler growth and body composition, Front Physiol. 13 (2022) 935311. https://doi.org/10.3389/fphys.2022.935311.
Crossref Google Scholar
[11]

N. Ma, X. Wang, Y. Qiao, et al., Coexpression of an intronic microRNA and its host gene reveals a potential role for miR-483-5p as an IGF2 partner, Mol. Cell Endocrinol. 333(1) (2011) 96–101. https://doi.org/10.1016/j.mce.2010.11.027.
Crossref Google Scholar
[12]

Z. Huang, S. Murphy, Increased intragenic IGF2 methylation is associated with repression of insulator activity and elevated expression in serous ovarian carcinoma, Front Oncol. 3 (2013) 131. https://doi.org/10.3389/fonc.2013.00131.
Crossref Google Scholar
[13]

G. A. Rohrer, D. J. Nonneman, R. K. Miller, et al., Association of single nucleotide polymorphism (SNP) markers in candidate genes and QTL regions with pork quality traits in commercial pigs, Meat Sci. 92(4) (2012) 511–518. https://doi.org/10.1016/j.meatsci.2012.05.020.
Crossref Google Scholar
[14]

Y. Gao, J. Jiang, S. Yang, et al., CNV discovery for milk composition traits in dairy cattle using whole genome resequencing, BMC Genomics 18(1) (2017) 265. https://doi.org/10.1186/s12864-017-3636-3.
Crossref Google Scholar
[15]

E. Bagnicka, E. Siadkowska, N. Strzałkowska, et al., Association of polymorphisms in exons 2 and 10 of the insulin-like growth factor 2 (IGF2) gene with milk production traits in Polish Holstein-Friesian cattle, J. Dairy Res. 77(1) (2010) 37–42. https://doi.org/10.1017/s0022029909990197.
Crossref Google Scholar
[16]

E. W. Berkowicz, D. A. Magee, K. M. Sikora, et al., Single nucleotide polymorphisms at the imprinted bovine insulin-like growth factor 2 (IGF2) locus are associated with dairy performance in Irish Holstein-Friesian cattle, J. Dairy Res. 78(1) (2011) 1–8. https://doi.org/10.1017/S0022029910000567.
Crossref Google Scholar
[17]

D. C. Park, S. Geun Yeo, E. Young Shin, et al., Clusterin confers paclitaxel resistance in cervical cancer, Gynecol. Oncol. 103(3) (2006) 996–1000. https://doi.org/10.1016/j.ygyno.2006.06.037.
Crossref Google Scholar
[18]

J. K. Shin, K. A. Han, M. Y. Kang, et al., Expression of clusterin in normal and preeclamptic placentas, J. Obstet. Gynaecol. Res. 34(4) (2008) 473–479. https://doi.org/10.1111/j.1447-0756.2008.00723.x.
Crossref Google Scholar
[19]

D. Bradley, A. Blaszczak, Z. Yin, et al., Clusterin impairs hepatic insulin sensitivity and adipocyte clusterin associates with cardiometabolic risk, Diabetes Care 42(3) (2019) 466–475. https://doi.org/10.2337/dc18-0870.
Crossref Google Scholar
[20]

Y. Yang, D. Bu, X. Zhao, et al., Proteomic analysis of cow, yak, buffalo, goat and camel milk whey proteins: quantitative differential expression patterns, J. Phys. Chem. Lett. 12(4) (2013) 1660–1667. https://doi.org/10.1021/pr301001m.
Crossref Google Scholar
[21]

C. Rodríguez-Rivera, M. M. Garcia, M. Molina-Álvarez, et al., Clusterin: always protecting. Synthesis, function and potential issues, Biomed. Pharmacother. 134 (2021) 111174. https://doi.org/10.1016/j.biopha.2020.111174.
Crossref Google Scholar
[22]

Z. Wang, J. Huang, J. Zhong, et al., Molecular cloning, promoter analysis, SNP detection of clusterin gene and their associations with mastitis in Chinese Holstein cows, Mol. Biol. Rep. 39(3) (2012) 2439–2445. https://doi.org/10.1007/s11033-011-0994-9.
Crossref Google Scholar
[23]

M. Nakamura, A. Tomita, H. Nakatani, et al., Antioxidant and antibacterial genes are upregulated in early involution of the mouse mammary gland: sharp increase of ceruloplasmin and lactoferrin in accumulating breast milk, DNA Cell Biol. 25(9) (2006) 491–500. https://doi.org/10.1089/dna.2006.25.491.
Crossref Google Scholar
[24]

E. Khalil, M. R. Digby, P. C. Thomson, et al., Acute involution in the tammar wallaby: identification of genes and putative novel milk proteins implicated in mammary gland function, Genomics 97(6) (2011) 372–378. https://doi.org/10.1016/j.ygeno.2011.03.003.
Crossref Google Scholar
[25]

A. Subramaniam, M. K. Shanmugam, E. Perumal, et al., Potential role of signal transducer and activator of transcription (STAT) 3 signaling pathway in inflammation, survival, proliferation and invasion of hepatocellular carcinoma, BBA-Reviews on Cancer 1835(1) (2013) 46–60. https://doi.org/10.1016/j.bbcan.2012.10.002.
Crossref Google Scholar
[26]

K. S. Siveen, S. Sikka, R. Surana, et al., Targeting the STAT3 signaling pathway in cancer: role of synthetic and natural inhibitors, BBA-Reviews on Cancer 1845(2) (2014) 136–154. https://doi.org/10.1016/j.bbcan.2013.12.005.
Crossref Google Scholar
[27]

Y. Verhoeven, S. Tilborghs, J. Jacobs, et al., The potential and controversy of targeting STAT family members in cancer, Semin. Cancer Biol. 60 (2020) 41–56. https://doi.org/10.1016/j.semcancer.2019.10.002.
Crossref Google Scholar
[28]

P. Brym, S. Kamiński, A. Ruść, New SSCP polymorphism within bovine STAT5A gene and its associations with milk performance traits in black-and-white and jersey cattle, J. Appl. Genet. 45(4) (2004) 445–452.
Crossref Google Scholar
[29]

M. Selvaggi, C. Dario, G. Normanno, et al., Genetic polymorphism of STAT5A protein: relationships with production traits and milk composition in Italian Brown cattle, J. Diary Res. 76(4) (2009) 441–445. https://doi.org/10.1017/S0022029909990070.
Crossref Google Scholar
[30]

K. Miyoshi, J. M. Shillingford, G. H. Smith, et al., Signal transducer and activator of transcription (Stat) 5 controls the proliferation and differentiation of mammary alveolar epithelium, J. Cell Biol. 155(4) (2001) 531–542. https://doi.org/10.1083/jcb.200107065.
Crossref Google Scholar
[31]

M. Selvaggi, S. Albarella, C. Dario, et al., Association of STAT5A gene variants with milk production traits in agerolese cattle, Biochem. Genet. 55(2) (2017) 158–167. https://doi.org/10.1007/s10528-016-9781-6.
Crossref Google Scholar
[32]

Y. F. Liu, J. L. Gao, Y. F. Yang, et al., Novel extraction method of genomic DNA suitable for long-fragment amplification from small amounts of milk, J. Dairy Sci. 97(11) (2014) 6804–6809. https://doi.org/10.3168/jds.2014-8066.
Crossref Google Scholar
[33]

Y. Liu, L. Zan, Y. Xin, et al., ZBTB38 gene polymorphism associated with body measurement traits in native Chinese cattle breeds, Gene 513 (2012) 272–277. https://doi.org/10.1016/j.gene.2012.10.026.
Crossref Google Scholar
[34]

R. Hovey, J. Harris, D. Hadsell, et al., Local insulin-like growth factor-II mediates prolactin-induced mammary gland development, Mol. Endocrinol. 17 (2003) 460–471. https://doi.org/10.1210/me.2002-0214.
Crossref Google Scholar
[35]

S. Ramadan, E. Manaa, M. Elatrouny, et al., Association of growth hormone (GH), insulin-like growth factor 2 (IGF2) and progesterone receptor (PGR) genes with some productive traits in Gabali rabbits, World Rabbit. Sci. 28 (2020) 135–144. https://doi.org/10.4995/wrs.2020.12610.
Crossref Google Scholar
[36]

G. M. Vacca, G. Stocco, M. L. Dettori, et al., Milk yield, quality, and coagulation properties of 6 breeds of goats: environmental and individual variability, J. Diary Sci. 101(8) (2018) 7236–7247. https://doi.org/10.3168/jds.2017-14111.
Crossref Google Scholar
[37]

L. French, J. Soriano, R. Montesano, et al., Modulation of clusterin gene expression in the rat mammary gland during pregnancy, lactation, and involution, Biol. Reprod. 55 (1997) 1213–1220. https://doi.org/10.1095/biolreprod55.6.1213.
Crossref Google Scholar
[38]

H. Khatib, R. L. Monson, V. Schutzkus, et al., Mutations in the STAT5A gene are associated with embryonic survival and milk composition in cattle, J. Diary Sci. 91(2) (2008) 784–793. https://doi.org/10.3168/jds.2007-0669.
Crossref Google Scholar
[39]

M. Sadeghi, M. M. Shahrbabak, G. R. Mianji, et al., Polymorphism at locus of STAT5A and its association with breeding values of milk production traits in Iranian Holstein bulls, Livest. Sci. 123(1) (2009) 97–100. https://doi.org/10.1016/j.livsci.2008.10.010.
Crossref Google Scholar
Food Science of Animal Products
Volume 1 Issue 1,
March 2023
Article number: 9240011
DOI: 10.26599/FSAP.2023.9240011
Cite this article:
Fu S, Ku T, Li L, et al. Association of polymorphisms in IGF2, CLU and STAT5A genes with milk production characteristics in Chinese Holstein cattle. Food Science of Animal Products, 2023, 1(1): 9240011. https://doi.org/10.26599/FSAP.2023.9240011

1651

Views

124

Downloads

0

Crossref

Altmetrics
Received: 10 February 2023
Revised: 17 March 2023
Accepted: 13 April 2023
Published: 22 May 2023
© Beijing Academy of Food Sciences 2023.

Food Science of Animal Products published by Tsinghua University Press. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
Return