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Research Article | Open Access

Antibacterial mechanisms, whole genome sequencing and potential functional prediction of Lactoplantibacillus plantarum L3

Hongyu Jin§, Yuhan Wang§, Jing Kong, Xiaoyun Han, Mengyang Li, Xiuliang Li, Qingshen Sun( )
Engineering Research Center of Agricultural Microbiology Technology, Ministry of Education & Heilongjiang Provincial Key Laboratory of Plant Genetic Engineering and Biological Fermentation Engineering for Cold Region & Key Laboratory of Microbiology, College of Heilongjiang Province & School of Life Sciences, Heilongjiang University, Harbin 150080, China

§These authors contributed equally to the work.

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Abstract

In our previous study, we have isolated Lactoplantibacillus plantarum L3 (GenBank accession No. MT781360) which can secret bacteriocin L3 with broad-spectrum antibacterial activities. This work aimed to illustrate the antibacterial mechanism of bacteriocin L3 and predict the functionalities of the L. plantarum L3 strain through whole genome analysis. Preliminary exploration of the bacteriostatic mechanism showed that bacteriocin L3 destroyed the cell membrane integrity and led to the extravasation of the cell contents, leading to cell death. The sequencing results showed that the genome of Lactobacillus plantarum L3 was 3 187 020 bp in size, with a GC content of 44.57%. Overall, 3 024 encoding genes were annotated, with a sequence length of 2 679 162 bp, accounting for 84.06% of the total genome length. The strain has strong reproductive and metabolic abilities and high safety. The bacteriocin gene cluster analysis showed that the bacteriocins L3 belonged to the class IIb bacteriocins. This work might provide a theoretical basis for the application of this strain in the food industry, especially its potential use in dairy products.

Keywords

Lactoplantibacillus plantarum L3bacteriocin L3bacteriostatic mechanismwhole genome sequencingfunctional prediction

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Food Science of Animal Products
Volume 1 Issue 2,
June 2023
Article number: 9240013
DOI: 10.26599/FSAP.2023.9240013
Cite this article:
Jin H, Wang Y, Kong J, et al. Antibacterial mechanisms, whole genome sequencing and potential functional prediction of Lactoplantibacillus plantarum L3. Food Science of Animal Products, 2023, 1(2): 9240013. https://doi.org/10.26599/FSAP.2023.9240013

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Received: 09 February 2023
Revised: 03 April 2023
Accepted: 10 April 2023
Published: 26 June 2023
© Beijing Academy of Food Sciences 2023.

Food Science of Animal Products published by Tsinghua University Press. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
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