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Research Article | Open Access

Whey protein hydrolysates alleviated food allergy in mice by balancing the Th1/Th2 pathway and increasing IgA antibody production

Xinkun Yin1, Xiaohong Guo2, Hui Hong1, Yongkang Luo1, Yuqing Tan1( )
Key Laboratory of Functional Dairy, China Agricultural University, College of Food Science and Nutritional Engineering, Beijing 100083, China
Xindongkang Nutrition Technology Co., Ltd., Shijiazhuang 052160, China
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Abstract

Whey protein, a by-product of cheese processing, is ubiquitously applied in infant formula. Nevertheless, it contains β-lactoglobulin, an allergenic component that can be enzymatically hydrolyzed to destroy its allergenic epitopes and antigenicity. The whey protein hydrolysate (WPH) obtained through enzymatic hydrolysis exhibits a wide range of biological activities. However, its utilization in preventing whey protein food allergies has received limited research attention. This study aimed to examine the preventive effect of WPH intervention on whey protein-induced food allergy in BALB/c mice. The results showed that WPH intervention notably mitigated the development of allergic reactions in mice with whey protein-induced food allergies. The intervention with WPH significantly reduced the symptom score of allergic reactions in mice with whey protein-induced food allergies (39.38%, P < 0.01). Early intervention with WPH also led to a significant reduction in the serum levels of antibodies and related cytokines, including IgE, histamine, IgG, and monocyte chemotactic protein-1 (MCP-1) in mice (P < 0.05). A potential mechanism for alleviating allergic reactions was identified from the proteomic findings. WPH was found to upregulate the Th1 differentiation pathway and IgA secretion pathway by increasing MHC II protein expression, thereby alleviating allergic reactions to whey protein in food. Regarding the gut microbiome, WPH intervention led to a decrease in the relative abundance of harmful bacteria, including Prevotellaceae_UCG-001, and Alloprevotella (5.4% and 2.3%, respectively; P< 0.05). It increased the relative abundance of beneficial bacteria such as Turicibacter, Roseburia, and Alistips (3.8%, 0.6%, and 1.4%, respectively; P < 0.05). The present study suggests early WPH intervention may attenuate whey protein-induced food allergic reactions.

Keywords

allergywhey protein hydrolysatespleen histopathology

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Food Science of Animal Products
Volume 1 Issue 4,
December 2023
Article number: 9240046
DOI: 10.26599/FSAP.2023.9240046
Cite this article:
Yin X, Guo X, Hong H, et al. Whey protein hydrolysates alleviated food allergy in mice by balancing the Th1/Th2 pathway and increasing IgA antibody production. Food Science of Animal Products, 2023, 1(4): 9240046. https://doi.org/10.26599/FSAP.2023.9240046

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Received: 08 November 2023
Revised: 01 December 2023
Accepted: 10 January 2024
Published: 04 March 2024
© Beijing Academy of Food Sciences 2023.

Food Science of Animal Products published by Tsinghua University Press. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
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