Isolation and identification, biological characterization of Staphylococcus aureus phage and its application in milk
), Junni Tang()Graphical Abstract
Abstract
Staphylococcus aureus is one of the important pathogens that cause food contamination worldwide, and poses a great danger to people. The common treatment is antibiotic therapy; however, the misuse of antibiotics has led to the continuous emergence of drug-resistant strains. Therefore, new antibacterial methods need to be explored. In this study, a lytic S. aureus phage (named SP-CmSa-11) was isolated from dairy farms which belongs to the Myoviridae phage family. The optimal multiple of infection of SP-CmSa-11 is 0.1, and the host range included 7 strains of mastitis cow-derived S. aureus, 12 strains of animal-origin methicillin-resistant S. aureus, 1 strain of S. xylosus, 1 strain of S. epidermidis and 1 strain of Enterococcus faecalis. SP-CmSa-11’s burst period was 40 min, and the burst size was about 130 PFU/cell. SP-CmSa-11 was inactivated after 10 min in a water bath at 70 °C, and the pH tolerance of SP-CmSa-11 ranges from 4 to 10 in 2 h. It’s insensitive to chloroform and ultraviolet radiation. The total genome length of SP-CmSa-11 is 45 816 bp. The G + C content is 27.23%, and has 59 putative open reading frames. SP-CmSa-11 showed good antibacterial effects in vitro and milk. These results suggest that SP-CmSa-11 may be a promising alternative therapy for food contamination caused by S. aureus.
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