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Research Article | Open Access

Effect of modified atmosphere packaging with high concentrations of CO2 on muscle degradation and endogenous enzyme activity of salmon fillets

Yunfang Qian1,2,3, Runjian Gao1, Chengcheng Liu1, Shengping Yang1,2,3()
College of Food Science & Technology, Shanghai Ocean University, Shanghai 201306, China
Shanghai Engineering Research Center of Aquatic Product Processing & Preservation, Shanghai 201306, China
National Experimental Teaching Demonstration Center for Food Science and Engineering (Shanghai Ocean University), Shanghai 201306, China
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Abstract

The preservation effect of modified atmosphere packaging (MAP) on seafood has been applied widely, but packaging with high concentrations of CO2 will have adverse effects on seafood. This study aimed to elucidate the potential mechanism of the adverse effects caused by MAP with high CO2 concentration at 4 °C. The changes in the quality of salmon fillets under high concentrations of CO2 packaging were demonstrated in this article through indicators such as total volatile basic nitrogen content, microbial counts, thiobarbituric acid reactive substances (TBARS) value, and drip loss. The underlying mechanisms were further explained from the perspectives of protein degradation and endogenous enzymes. The study indicated that the microbial counts in the 90% CO2/10% O2 group were higher than both the 75% CO2/10% O2/15% N2 and 60% CO2/10% O2/30% N2 groups on the 12th day, and there was also an increase in TBARS value and drip loss. On the 2nd day, the caspase-3 relative activity in the 90% CO2/10% O2 group (119.96%) was found to be higher than the 75% CO2/10% O2/15% N2 group (111.99%). On the 12th day, the relative activities of caspase-3 in the 90% CO2/10% O2 group and the 75% CO2/10% O2/15% N2 group were 34.13% and 25.77%, respectively, the latter exhibited the lowest cathepsin activity and the minimum myofibril fragmentation index. The results of free amino acids analysis indicated that the 90% CO2/10% O2 group was also accompanied by an accumulation of more bitter amino acids during storage. The MAP with 75% CO2 had better effect on inhibiting caspases and cathepsins, which could be the main reason to a better muscle quality of salmon fillets at 4 °C.

Keywords

salmonmodified atmosphere packagingprotein degradationcathepsincaspase

References

[1]

S. Redfern, M. Dermiki, S. Fox, et al., The effects of cooking salmon sous-vide on its antithrombotic properties, lipid profile and sensory characteristics, Food Res. Int. 139 (2021) 109976. https://doi.org/10.1016/j.foodres.2020.109976.

Crossref Google Scholar
[2]

Y. F. Qian, C. C. Liu, J. J. Zhang, et al., Effects of modified atmosphere packaging with varied CO2 and O2 concentrations on the texture, protein, and odor characteristics of salmon during cold storage, Foods 1122 (2022) 3560. https://doi.org/10.3390/foods11223560.

Crossref Google Scholar
[3]

Z. J. Yang, J. Yan, J. Xie, Effect of vacuum and modified atmosphere packaging on moisture state, quality, and microbial communities of grouper ( Epinephelus coioides) fillets during cold storage, Food Res. Int. 173(Pt 1) (2023) 113340. https://doi.org/10.1016/j.foodres.2023.113340.

Crossref Google Scholar
[4]

X. B. Nie, R. C. Zhang, L. L. Cheng, et al., Mechanisms underlying the deterioration of fish quality after harvest and methods of preservation, Food Control 135 (2022) 108805. https://doi.org/10.1016/j.foodcont.2021.108805.

Crossref Google Scholar
[5]

E. Esteves, L. Guerra, J. Aníbal, Effects of vacuum and modified atmosphere packaging on the quality and shelf-life of gray triggerfish ( Balistes capriscus) fillets, Foods 102 (2021) 250. https://doi.org/10.3390/foods10020250.

Crossref Google Scholar
[6]

Y. Lei, Y. Zhang, Y. Cheng, et al., Monitoring and identification of spoilage-related microorganisms in braised chicken with modified atmosphere packaging during refrigerated storage, Food Sci. Hum. Wellness 121 (2023) 28–34. https://doi.org/10.1016/j.fshw.2022.07.015.

Crossref Google Scholar
[7]

J. Yang, X. Y. Yang, R. R. Liang, et al., The response of bacterial communities to carbon dioxide in high-oxygen modified atmosphere packaged beef steaks during chilled storage, Food Res. Int. 151 (2022) 110872. https://doi.org/10.1016/j.foodres.2021.110872.

Crossref Google Scholar
[8]

J. B. Zhang, Y. Li, X. C. Liu, et al., Characterization of the microbial composition and quality of lightly salted grass carp ( Ctenopharyngodon idellus) fillets with vacuum or modified atmosphere packaging, Int. J. Food Microbiol. 293 (2019) 87–93. https://doi.org/10.1016/j.ijfoodmicro.2018.12.022.

Crossref Google Scholar
[9]

P. Y. Li, S. Y. Fang, J. B. Wang, et al., Influence of high CO2 modified atmosphere packaging on some quality characteristics of fresh farmed pufferfish ( Takifugu obscurus) during refrigerated storage, Czech J. Food Sci. 382 (2020) 123–130. https://doi.org/10.17221/188/2019-cjfs.

Crossref Google Scholar
[10]

G. Bono, C. Badalucco, S. Cusumano, et al., Toward shrimp without chemical additives: a combined freezing-MAP approach, LWT-Food Sci. Technol. 46 (2012) 274–279. https://doi.org/10.1016/j.lwt.2011.09.020.

Crossref Google Scholar
[11]

O. Sørheim, R. Ofstad, P. Lea, Effects of carbon dioxide on yield, texture and microstructure of cooked ground beef, Meat Sci. 67 (2004) 231–236. https://doi.org/10.1016/j.meatsci.2003.10.010.

Crossref Google Scholar
[12]

N. Li, J. Xie, Y. M. Chu, Degradation and evaluation of myofibril proteins induced by endogenous protease in aquatic products during storage: a review, Food Sci. Biotechnol. 328 (2023) 1005–1018. https://doi.org/10.1007/s10068-023-01291-4.

Crossref Google Scholar
[13]

M. A. Sentandreu, G. Coulis, A. Ouali, Role of muscle endopeptidases and their inhibitors in meat tenderness, Trends Food Sci. Technol. 1312 (2002) 400–421. https://doi.org/10.1016/s0924-2244(02)00188-7.

Crossref Google Scholar
[14]

M. H. Zhang, D. Y. Wang, W. Huang, et al., Apoptosis during postmortem conditioning and its relationship to duck meat quality, Food Chem. 1381 (2013) 96–100. https://doi.org/10.1016/j.foodchem.2012.10.142.

Crossref Google Scholar
[15]

C. M. Kemp, R. G. Bardsley, T. Parr, Changes in caspase activity during the postmortem conditioning period and its relationship to shear force in porcine longissimus muscle, J. Anim. Sci. 8410 (2006) 2841–2846. https://doi.org/10.2527/jas.2006-163.

Crossref Google Scholar
[16]

C. M. Kemp, T. Parr, Advances in apoptotic mediated proteolysis in meat tenderisation, Meat Sci. 923 (2012) 252–259. https://doi.org/10.1016/j.meatsci.2012.03.013.

Crossref Google Scholar
[17]

Y. J. Yu, S. P. Yang, T. Lin, et al., Effect of cold chain logistic interruptions on lipid oxidation and volatile organic compounds of salmon ( Salmo salar) and their correlations with water dynamics, Front. Nutr. 7 (2020) 155. https://doi.org/10.3389/fnut.2020.00155.

Crossref Google Scholar
[18]

Z. Wang, S. F. Hu, Y. P. Gao, et al., Effect of collagen-lysozyme coating on fresh-salmon fillets preservation, LWT-Food Sci. Technol. 75 (2017) 59–64. https://doi.org/10.1016/j.lwt.2016.08.032.

Crossref Google Scholar
[19]

J. Xue, D. Wang, S. Zhang, et al., Analysis of changes in low-salt conditioned grass carp ( Ctenopharyngodon idella) fillets during refrigeration in terms of quality and protein stability, Food Sci. Anim. Prod. 1 (2023) 9240028. https://doi.org/10.26599/FSAP.2023.9240028.

Crossref Google Scholar
[20]

X. Y. Sun, X. B. Guo, M. Y. Ji, et al., Preservative effects of fish gelatin coating enriched with CUR/ βCD emulsion on grass carp ( Ctenopharyngodon idellus) fillets during storage at 4 °C, Food Chem. 272 (2019) 643–652. https://doi.org/10.1016/j.foodchem.2018.08.040.

Crossref Google Scholar
[21]

L. M. Zhang, D. W. Yu, Y. S. Xu, et al., The inhibition mechanism of nanoparticles-loading bilayer film on texture deterioration of refrigerated carp fillets from the perspective of protein changes and exudates, Food Chem. 424 (2023) 136440. https://doi.org/10.1016/j.foodchem.2023.136440.

Crossref Google Scholar
[22]

J. H. Li, J. Y. Shi, X. W. Huang, et al., Effects of pulsed electric field on freeze-thaw quality of Atlantic salmon, Innov. Food Sci. Emerg. Technol. 65 (2020) 102454. https://doi.org/10.1016/j.ifset.2020.102454.

Crossref Google Scholar
[23]

A. Konosu, K. Watanabe, T. Shimizu, Distribution of nitrogenous constituents in the muscle extracts of eight species of fish, Nippon Suisan Gakkaishi 409 (1974) 909–915. https://doi.org/10.2331/suisan.40.909.

Crossref Google Scholar
[24]

B. Zou, Changes of postmortem apoptotic factors, genes and proteins and their potential associations with beef tenderization, Food Sci. Anim. Prod. 1 (2023) 9240040. https://doi.org/10.26599/FSAP.2023.9240040.

Crossref Google Scholar
[25]

J. Chen, H. Ye, H. Li, et al., Effects of temperature fluctuations on the quality and characteristic volatile compounds of large yellow croaker ( Pseudosciaena crocea) during cold chain logistics, Food Sci. Anim. Prod. 2 (2024) 9240057. https://doi.org/10.26599/FSAP.2023.9240057.

Crossref Google Scholar
[26]

L. Gram, H. H. Huss, Microbiological spoilage of fish and fish products, Int. J. Food Microbiol. 331 (1996) 121–137. https://doi.org/10.1016/0168-1605(96)01134-8.

Crossref Google Scholar
[27]

Z. Jin, J. Li, J. Wang, et al., Applying different methods to evaluate the freshness of large yellow croacker ( Pseudosciaena crocea) fillets during chilled storage, J. Agric. Food Chem. 60 (2012) 11387–11394. https://doi.org/10.1021/jf303439p.

Crossref Google Scholar
[28]

K. Fernández, E. Aspe, M. Roeckel, Shelf-life extension on fillets of Atlantic salmon ( Salmo salar) using natural additives, superchilling and modified atmosphere packaging, Food Control 2011 (2009) 1036–1042. https://doi.org/10.1016/j.foodcont.2008.12.010.

Crossref Google Scholar
[29]

Y. F. Qian, J. Xie, S. P. Yang, et al., Inhibitory effect of a quercetin-based soaking formulation and modified atmospheric packaging (MAP) on muscle degradation of Pacific white shrimp ( Litopenaeus vannamei), LWT-Food Sci. Technol. 632 (2015) 1339–1346. https://doi.org/10.1016/j.lwt.2015.03.077.

Crossref Google Scholar
[30]

L. A. Ouahioune, M. Wrona, C. Nerín, et al., Novel active biopackaging incorporated with macerate of carob ( Ceratonia siliqua L.) to extend shelf-life of stored Atlantic salmon fillets ( Salmo salar L.), LWT-Food Sci. Technol. 156 (2022) 12. https://doi.org/10.1016/j.lwt.2021.113015.

Crossref Google Scholar
[31]

Y. Li, J. Q. Huang, C. H. Yuan, et al., Developing a new spoilage potential algorithm and identifying spoilage volatiles in small yellow croaker ( Larimichthys polyactis) under vacuum packaging condition, LWT-Food Sci. Technol. 106 (2019) 209–217. https://doi.org/10.1016/j.lwt.2019.02.075.

Crossref Google Scholar
[32]

P. Y. Li, J. Mei, M. T. Tan, et al., Effect of CO2 on the spoilage potential of Shewanella putrefaciens target to flavour compounds, Food Chem. 397 (2022) 133748. https://doi.org/10.1016/j.foodchem.2022.133748.

Crossref Google Scholar
[33]

A. A. Gonçalves, T. C. L. Santos, Improving quality and shelf-life of whole chilled Pacific white shrimp ( Litopenaeus vannamei) by ozone technology combined with modified atmosphere packaging, LWT-Food Sci. Technol. 99 (2019) 568–575. https://doi.org/10.1016/j.lwt.2018.09.083.

Crossref Google Scholar
[34]

Y. W. Chen, W. Q. Cai, Y. G. Shi, et al., Effects of different salt concentrations and vacuum packaging on the shelf-stability of Russian sturgeon ( Acipenser gueldenstaedti) stored at 4 °C, Food Control 109 (2020) 106865. https://doi.org/10.1016/j.foodcont.2019.106865.

Crossref Google Scholar
[35]

H. M. Tie, J. L. Dong, Q. X. Jiang, et al., Profound changes of mitochondria during postmortem condition used as freshness indicator in grass carp ( Ctenopharyngodon idella) muscle, Food Biosci. 48 (2022) 101749. https://doi.org/10.1016/j.fbio.2022.101749.

Crossref Google Scholar
[36]

K. Fischer, Drip loss in pork: influencing factors and relation to further meat quality traits, J. Anim. Breed. Genet. 124(Suppl 1) (2007) 12–18. https://doi.org/10.1111/j.1439-0388.2007.00682.x.

Crossref Google Scholar
[37]

R. Thepnuan, S. Benjakul, W. Visessanguan, Effect of pyrophosphate and 4-hexylresorcinol pretreatment on quality of refrigerated white shrimp ( Litopenaeus vannamei) kept under modified atmosphere packaging, J. Food Sci. 733 (2008) S124–S133. https://doi.org/10.1111/j.1750-3841.2008.00674.x.

Crossref Google Scholar
[38]

R. Mahto, S. Ghosh, M. K. Das, et al., Effect of gamma irradiation and frozen storage on the quality of fresh water prawn ( Macrobrachium rosenbergii) and tiger prawn ( Penaeus monodon), LWT-Food Sci. Technol. 612 (2015) 573–582. https://doi.org/10.1016/j.lwt.2014.12.028.

Crossref Google Scholar
[39]

N. Li, Y. Shen, W. R. Liu, et al., Low-field NMR and MRI to analyze the effect of edible coating incorporated with MAP on qualities of half-smooth tongue sole ( Cynoglossus semilaevis Gunther) fillets during refrigerated storage, Appl. Sci. 88 (2018) 1391. https://doi.org/10.3390/app8081391.

Crossref Google Scholar
[40]

L. Zhang, M. Y. Yin, X. C. Wang, Meat texture, muscle histochemistry and protein composition of Eriocheir sinensis with different size traits, Food Chem. 338 (2021) 127632. https://doi.org/10.1016/j.foodchem.2020.127632.

Crossref Google Scholar
[41]

H. Vasconcelos, J. de Almeida, A. Matias, et al., Detection of biogenic amines in several foods with different sample treatments: an overview, Trends Food Sci. Technol. 113 (2021) 86–96. https://doi.org/10.1016/j.jpgs.2021.04.043.

Crossref Google Scholar
[42]

L. A. Volpelli, S. Failla, A. Sepulcri, et al., Calpain system in vitro activity and myofibril fragmentation index in fallow deer ( Dama dama): effects of age and supplementary feeding, Meat Sci. 693 (2005) 579–582. https://doi.org/10.1016/j.meatsci.2004.09.009.

Crossref Google Scholar
[43]
Y. Zhao, C. Xiang, B. Roy, et al., Apoptosis and its role in postmortem meat tenderness: a comprehensive review, Meat Sci. 219 (2024) 109652. 10.1016/j. meatsci. 2024.109652.
Crossref
[44]

M. Huang, F. Huang, M. Xue, et al., The effect of active caspase-3 on degradation of chicken myofibrillar proteins and structure of myofibrils, Food Chem. 1281 (2011) 22–27. https://doi.org/10.1016/j.foodchem.2011.02.062.

Crossref Google Scholar
[45]

E. M. Kim, E. J. Shin, J. A. Lee, et al., Caspase-9 activation and APAF-1 cleavage by MMP-3, Biochem. Biophys. Res. Commun. 4533 (2014) 563–568. https://doi.org/10.1016/j.bbrc.2014.09.124.

Crossref Google Scholar
[46]

X. Xin, D. Ning, S. Zhuang, et al., Caspase-3 interactions with calpain and cathepsin L: implications for protein stability and quality in fish fillets during postmortem storage, Food Biosci. 61 (2024) 104709. https://doi.org/10.1016/j.fbio.2024.104709.

Crossref Google Scholar
[47]

D. W. Yu, W. Y. Zhao, X. Y. Wan, et al., The protective pattern of chitosan-based active coating on texture stabilization of refrigerated carp fillets from the perspective of proteolysis, Food Chem. 404 (2023) 134633. https://doi.org/10.1016/j.foodchem.2022.134633.

Crossref Google Scholar
[48]
C. Ladrat, V. Verrez-Bagnis, J. Noël, et al., In vitro proteolysis of myofibrillar and sarcoplasmic proteins of white muscle of sea bass (Dicentrarchus labrax L. ): effects of cathepsins B, D and L, Food Chem. 814 (2003) 517–525. https://doi.org/10.1016/s0308-8146(02)00481-8.
Crossref
[49]

H. H. Qiu, X. Guo, X. R. Deng, et al., The influence of endogenous cathepsin in different subcellular fractions on the quality deterioration of Northern pike ( Esox lucius) fillets during refrigeration and partial freezing storage, Food Sci. Biotechnol. 2910 (2020) 1331–1341. https://doi.org/10.1007/s10068-020-00781-z.

Crossref Google Scholar
Food Science of Animal Products
Volume 3 Issue 1,
March 2025
Article number: 9240103
DOI: 10.26599/FSAP.2025.9240103
Cite this article:
Qian Y, Gao R, Liu C, et al. Effect of modified atmosphere packaging with high concentrations of CO2 on muscle degradation and endogenous enzyme activity of salmon fillets. Food Science of Animal Products, 2025, 3(1): 9240103. https://doi.org/10.26599/FSAP.2025.9240103
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