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Research Article | Open Access

Dynamic changes in physicochemical property, biogenic amines content and microbial diversity during the fermentation of Sanchuan ham

Zhongai Chena,b,, Hong Chena, Huan Dua, Cong Chena, Kaixiang Lua, Qiaoli Xuec( ), Yongjin Hua( )
College of Food Science and Technology, Yunnan Agricultural University, Kunming 650000, China
Institute of Food Processing, Guizhou Academy of Agricultural Sciences, Guiyang 550006, China
Editorial Department of Journal of Yunnan Agricultural University, Yunnan Agricultural University, Kunming 650000, China

Peer review under responsibility of Tsinghua University Press.

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Abstract

Sanchuan ham is appreciated in Yunnan Province, China, for its characteristic flavor and taste, while the microbial community structure and biogenic amines content remain unclear during fermentation processes. In this study, we explored the physicochemical property, biogenic amines concentration and microbial diversity of external and internal Sanchuan ham by high-throughput sequencing during the processing of Sanchuan ham. Results showed that the nitrite remained at a stable level of 0.15 mg/kg which was significantly lower than the national health standard safety level of 20 mg/kg. In addition, compared with fresh hams, the content of total free amino acids in ripe Sanchuan ham has grown 14 folds; sour and bitter were the main tastes of Sanchuan ham. Notably, the concentration of cadaverine was the highest of all biogenic amines during the entire fermentation period. At the bacterial phyla level, Firmicutes and Actinobacteria were the two main phyla, while at the genus level, Staphylococcus was a significant strain throughout the whole fermentation. Moreover, the dry stage has a great impact on the succession change of microbial community structure. Simultaneously, the change trends and composition of bacteria in the interior have slight discrepancies with those of the exterior of Sanchuan ham.

Keywords

Sanchuan hamNitrite contentFree amino acidsCadaverineStaphylococcus

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References

[1]

Y. Wang, F. Li, J. Chen, et al., High-throughput sequencing-based characterization of the predominant microbial community associated with characteristic flavor formation in Jinhua Ham, Food Microbiol. 94 (2021) 103643-103652. https://doi.org/10.1016/j.fm.2020.103643.
Crossref Google Scholar
[2]

H. Li, J. Wu, J. Wan, et al., Extraction and identification of bioactive peptides from Panxian dry-cured ham with multifunctional activities, LWT-Food Sci. Technol. 160 (2022) 113326-113335. https://doi.org/10.1016/j.lwt.2022.113326.
Crossref Google Scholar
[3]

Y. Zhu, Y. Guo, F. Yang, et al., Combined application of high-throughput sequencing and UHPLC-Q/TOF-MS-based metabolomics in the evaluation of microorganisms and metabolites of dry-cured ham of different origins, Int. J. Food Microbiol. 359 (2021) 109422-109434. https://doi.org/10.1016/j.ijfoodmicro.2021.109422.
Crossref Google Scholar
[4]

S. Liu, G. Wang, Z. Xiao, et al., 1H-NMR-based water-soluble low molecular weight compound characterization and free fatty acid composition of five kinds of Yunnan dry-cured hams, LWT-Food Sci. Technol. 108 (2019) 174-182. https://doi.org/10.1016/j.lwt.2019.03.043.
Crossref Google Scholar
[5]

X. Ding, G. Wang, Y. Zou, et al., Evaluation of small molecular metabolites and sensory properties of Xuanwei ham salted with partial replacement of NaCl by KCl, Meat Sci. 175 (2021) 108465-108476. https://doi.org/10.1016/j.meatsci.2021.108465.
Crossref Google Scholar
[6]

Y.H. Zhang, B. Shan, J.S. Gong, et al., Mechanism of biogenic amine synthesis of Enterococcus faecium isolated from Sanchun ham, Food Sci. Nutr. 00 (2022) 1-14. https://doi.org/10.1002/fsn3.2820.
Crossref Google Scholar
[7]

W.Q. Li, Y.P. Chen, I. Blank, et al., GC×GC-ToF-MS and GC-IMS based volatile profile characterization of the Chinese dry-cured hams from different regions, Food Res. Int. 142 (2021) 110222-110230. https://doi.org/10.1016/j.foodres.2021.110222.
Crossref Google Scholar
[8]

G. Landeta, B. Rivas, A. V. Carrascosa, et al., Screening of biogenic amine production by coagulase-negative staphylococci isolated during industrial Spanish dry-cured ham processes, Meat Sci. 77(4) (2007) 556-561. https://doi.org/10.1016/j.meatsci.2007.05.004.
Crossref Google Scholar
[9]

C. M. Alfaia, M. F. Castro, V. A. Reis, et al., Changes in the profile of free amino acids and biogenic amines during the extended short ripening of Portuguese dry-cured ham, Food Sci. Technol. Int. 10(5) (2016) 297-304. https://doi.org/10.1177/1082013204047597.
Crossref Google Scholar
[10]

M. Flores, F.Toldrá, Microbial enzymatic activities for improved fermented meats, Trends Food Sci. Technol. 22(2/3) (2011) 81-90.
Crossref Google Scholar
[11]

Y. Mu, W. Su, Y. Mu, et al., Combined application of high-throughput sequencing and metabolomics reveals metabolically active microorganisms during panxian ham processing, Front Microbiol. 10 (2019) 3012-3024. https://doi.org/10.3389/fmicb.2019.03012.
Crossref Google Scholar
[12]

A. Zhong, W. Chen, Y. Duan, et al., The potential correlation between microbial communities and flavors in traditional fermented sour meat, LWT-Food Sci. Technol. 149 (2021) 111873-111888. https://doi.org/10.1016/j.lwt.2021.111873.
Crossref Google Scholar
[13]

W. Dong, H. Shen, H. Liu, et al., Unraveling the microbial community and succession during zha-chili fermentation and their relationships with flavor formation, Food Res Int. 157 (2022) 111239-111249. https://doi.org/10.1016/j.foodres.2022.111239.
Crossref Google Scholar
[14]

G.L. Tan, M. Hu, X.Y. Li, et al., High-throughput sequencing and metabolomics reveal differences in bacterial diversity and metabolites between red and white sufu, Front Microbiol. 11 (2020) 758-770. https://doi.org/10.3389/fmicb.2020.00758.
Crossref Google Scholar
[15]

C. Roseiro, C. Santos, M. Sol, et al., Prevalence of biogenic amines during ripening of a traditional dry fermented pork sausage and its relation to the amount of sodium chloride added, Meat Sci. 74(3) (2006) 557-563. https://doi.org/10.1016/j.meatsci.2006.03.030.
Crossref Google Scholar
[16]

M. Yu, X. Xiang, H. Tan, et al., Potential correlation between volatiles and microbiome of Xiang xi sausages from four different regions, Food Res. Int. 139(2) (2021) 109943-109953. https://doi.org/10.1016/j.foodres.2020.109943.
Crossref Google Scholar
[17]

Z.H. Wang, Z. Xu, L.M. Sun, et al., Dynamics of microbial communities, texture and flavor in Suan zuo yu during fermentation, Food Chem. 332 (2020) 127364-127371. https://doi.org/10.1016/j.foodchem.2020.127364.
Crossref Google Scholar
[18]

R. Ramirez, R. Contador, A. Ortiz, et al., Effect of breed purity and rearing systems on the stability of sliced Iberian dry-cured ham stored in modified atmosphere and vacuum packaging, Foods, 10 (2021) 730-749. https://doi.org/10.3390/foods10040730.
Crossref Google Scholar
[19]

N. Martinez-Onandi, C. Sanchez, M. Nunez, et al., Microbiota of Iberian dry-cured ham as influenced by chemical composition, high pressure processing and prolonged refrigerated storage, Food Microbiol. 80 (2019) 62-69. https://doi.org/10.1016/j.fm.2019.01.002.
Crossref Google Scholar
[20]

M. Laranjo, A. Gomes, A.C. Agulheiro-Santos, et al., Impact of salt reduction on biogenic amines, fatty acids, microbiota, texture and sensory profile in traditional blood dry-cured sausages, Food Chem. 218 (2017) 129-136. https://doi.org/10.1016/j.foodchem.2016.09.056.
Crossref Google Scholar
[21]

W. Cai, F. Tang, Y. Wang, et al., Bacterial diversity and flavor profile of Zha-Chili, a traditional fermented food in China, Food Res Int. 141(1) (2021) 110112-110124. https://doi.org/10.1016/j.foodres.2021.110112.
Crossref Google Scholar
[22]

X. Chen, J. Li, T. Zhou, et al., Two efficient nitrite-reducing Lactobacillus strains isolated from traditional fermented pork (Nanx Wudl) as competitive starter cultures for Chinese fermented dry sausage, Meat Sci. 121 (2016) 302-309. https://doi.org/10.1016/j.meatsci.2016.06.007.
Crossref Google Scholar
[23]

F. Toldra, M.C. Aristoy, M. Flores, et al., Contribution of muscle aminopeptidases to flavor development in dry-cured ham, Food Res. Int. 33 (2000) 181-185. https://doi.org/10.1016/S0963-9969(00)00032-6.
Crossref Google Scholar
[24]

X.N. Huang, S.Z. Yu, B.Z. Han, et al., Bacterial community succession and metabolite changes during sufu fermentation, LWT-Food Sci. Technol. 97 (2018) 537-545. https://doi.org/10.1016/j.lwt.2018.07.041.
Crossref Google Scholar
[25]

Y. Ardo, Flavour formation by amino acid catabolism, Biotechnol Adv. 24(2) (2006) 238-242. https://doi.org/10.1016/j.biotechadv.2005.11.005.
Crossref Google Scholar
[26]

F. Xiao, S. Hang, Y. Zhou, et al., Bromelain kinetics and mechanism on myofibril from golden pomfret (Trachinotus blochii), J. Food Sci. 83(8) (2018) 2148-2158.
Crossref Google Scholar
[27]

P. Liu, Q. Xiang, G. Chen, et al., Consumer preference of Chinese traditional fermented fava pastes, Int. J. Food Prop. 21(1) (2018) 2469-2490. https://doi.org/10.1080/10942912.2018.1528271.
Crossref Google Scholar
[28]

L. Martin, T. Antequera, J. Ventanas, et al., Free amino acids and other non-volatile compounds formed during processing of Iberian ham, Meat Sci. 59(4) (2001) 363-368.
Crossref Google Scholar
[29]

D. Ly, S. Mayrhofer, J.M. Schmidt, et al., Biogenic amine contents and microbial characteristics of cambodian fermented foods, Foods 9(2) (2020) 198-217. https://doi.org/10.3390/foods9020198.
Crossref Google Scholar
[30]

P. Kalac, Biologically active polyamines in beef, pork and meat products: a review, Meat Sci. 73(1) (2006) 1-11. https://doi.org/10.1016/j.meatsci.2005.11.001.
Crossref Google Scholar
[31]

M. Hu, J. Dong, G.L. Tan, et al., Metagenomic insights into the bacteria responsible for producing biogenic amines in sufu, Food Microbiol. 98(8) (2021) 103762-103771. https://doi.org/10.1016/j.fm.2021.103762.
Crossref Google Scholar
[32]

J. Zang, Y. Xu, W. Xia, et al., Dynamics and diversity of microbial community succession during fermentation of Suan yu, a Chinese traditional fermented fish, determined by high throughput sequencing, Food Res Int. 111 (2018) 565-573. https://doi.org/10.1016/j.foodres.2018.05.076.
Crossref Google Scholar
[33]

Y. Hu, L. Zhang, Q. Liu, et al., The potential correlation between bacterial diversity and the characteristic volatile flavour of traditional dry sausages from Northeast China, Food Microbiol. 91 (2020) 103505-103516. https://doi.org/10.1016/j.fm.2020.103505.
Crossref Google Scholar
[34]

X. Wang, Y. Zhang, H. Ren, et al., Comparison of bacterial diversity profiles and microbial safety assessment of salami, Chinese dry-cured sausage and Chinese smoked-cured sausage by high-throughput sequencing, LWT-Food Sci. Technol. 90 (2018) 108-115. https://doi.org/10.1016/j.lwt.2017.12.011.
Crossref Google Scholar
[35]

R. Haba, C. López-Hermoso, C. Sánchez-Porro, et al., Comparative genomics and phylogenomic analysis of the genus salinivibrio, Front Microbiol. 10 (2019) 2104.
Crossref Google Scholar
[36]

D. Zhao, J. Hu. W. Chen, Analysis of the relationship between microorganisms and flavour development in dry-cured grass carp by high-throughput sequencing, volatile flavour analysis and metabolomics, Food Chem. 368 (2022) 130889-130901. https://doi.org/10.1016/j.foodchem.2021.130889.
Crossref Google Scholar
[37]

H. Li, Z. Li, J. Qu, et al., Bacterial diversity in traditional Jiaozi and sourdough revealed by high-throughput sequencing of 16S rRNA amplicons, LWT-Food Sci. Technol. 81 (2017) 319-325. https://doi.org/10.1016/j.lwt.2017.04.007.
Crossref Google Scholar
[38]

Q. Guan, T. Huang, F. Peng, et al., The microbial succession and their correlation with the dynamics of flavor compounds involved in the natural fermentation of suansun, a traditional Chinese fermented bamboo shoots, Food Res. Int. 157 (2022) 111216-111225. https://doi.org/10.1016/j.foodres.2022.111216.
Crossref Google Scholar
[39]

X. Zheng, F. Liu, K. Li, et al., Evaluating the microbial ecology and metabolite profile in Kazak artisanal cheeses from Xinjiang, China, Food Res. Int. 111 (2018) 130-136. https://doi.org/10.1016/j.foodres.2018.05.019.
Crossref Google Scholar
[40]

D. Yao, L. Xu, M.N. Wu, et al., Effects of microbial community succession on flavor compounds and physicochemical properties during CS sufu fermentation, LWT-Food Sci. Technol. 152 (2021) 112313-112325. https://doi.org/10.1016/j.lwt.2021.112313.
Crossref Google Scholar
Food Science and Human Wellness
Volume 13 Issue 1,
January 2024
Pages 506-516
DOI: 10.26599/FSHW.2022.9250044
Cite this article:
Chen Z, Chen H, Du H, et al. Dynamic changes in physicochemical property, biogenic amines content and microbial diversity during the fermentation of Sanchuan ham. Food Science and Human Wellness, 2024, 13(1): 506-516. https://doi.org/10.26599/FSHW.2022.9250044

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Received: 24 June 2022
Revised: 18 July 2022
Accepted: 01 August 2022
Published: 01 June 2023
© 2024 Beijing Academy of Food Sciences. Publishing services by Tsinghua University Press.

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
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