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Open Access

Demonstration of safety characteristics and effects on gut microbiota of Lactobacillus gasseri HMV18

Xianxian Jiaa,b,1, Miaomiao Jiab,c,1, Xiang Gaoa,b,1, Xiang Lic, Mengyuan Wangc, Shengqiang Duc, Rui Huangc, Xiaotong Lic, Jun Zhangc, Shujin Lib,d, Chunling Mab,d, Yan Zhangb,e( ), Bin Congb,d( )
Department of Pathogen Biology, Institute of basic Medicine, Hebei Medical University, Shijiazhuang 050017, China
Research Unit of Digestive Tract Microecosystem Pharmacology and Toxicology, Chinese Academy of Medical Sciences, Shijiazhuang 050017, China
Institute of Basic Medicine, Hebei Medical University, Shijiazhuang 050017, China
College of Forensic Medicine, Hebei Key Laboratory of Forensic Medicine, Collaborative Innovation Center of Forensic Medical Molecular Identification, Hebei Medical University, Shijiazhuang 050017, China
Hebei Food Safety Key Laboratory, Hebei Food Inspection and Research Institute, Shijiazhuang 050227, China

1 These authors contributed equally to this work.

Peer review under responsibility of Tsinghua University Press.

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Highlights

• Lactobacillus gasseri HMV18 is a potential probiotic.

• Bioamine, mucin utilization and genome encoded mammalian toxin are negative.

• Mice orally administrated HMV18 do no observable harm to organs but can affect the structure and composition of gut microbiota.

• We offered a route map for design safety evaluation experiments.

Abstract

Human normal flora is a source of probiotics. The safety characteristics of a specific isolate determine its application in foods or drugs. The food-borne-pathogen antagonist strain Lactobacillus gasseri HMV18 is one of the isolates from normal human f lora. In this work, we assessed the in vitro pH tolerance, bile tolerance, biogenic amine production, mucin utilization, and safety of in vivo administration to mice to evaluate general health, organ-body weight index, organ histopathological change, whether L. gasseri HMV18 can colonize in the gut or modulate the gut microbiota after oral administration. The results suggest that L. gasseri HMV18 can tolerate pH 3 for 2 h, 3% bile for 3 h, biogenic amine negative, mucin usage negative, does not encode verif ied toxins, and cause no visible change in mice’s organs. L. gasseri HMV18 might not colonize in mice’s gut, but can signif icantly affect the structure of gut microbiota. A bibliographical survey suggested that there were as few as 8 opportunistic infection cases from 1984 to 2022 and that the possibility for L. gasseri to cause infection is relatively low. Therefore, this work provides a basis for the foods or drugs application of L. gasseri HMV18 and gives a map of experiments for the safety assessment of probiotics.

Keywords

ProbioticsLactobacillus gasseriSafety assessmentGut microbiota

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Food Science and Human Wellness
Volume 13 Issue 2,
March 2024
Pages 611-620
DOI: 10.26599/FSHW.2022.9250052
Cite this article:
Jia X, Jia M, Gao X, et al. Demonstration of safety characteristics and effects on gut microbiota of Lactobacillus gasseri HMV18. Food Science and Human Wellness, 2024, 13(2): 611-620. https://doi.org/10.26599/FSHW.2022.9250052

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Received: 13 February 2023
Revised: 26 February 2023
Accepted: 04 March 2023
Published: 25 September 2023
© 2024 Beijing Academy of Food Sciences. Publishing services by Tsinghua University Press.

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
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